;PMID: 1874450 ;source_file_2860.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..32] = [t:0..32] ;1)sentence:[e:38..146] = [t:38..146] ;2)section:[e:150..174] = [t:150..174] ;3)section:[e:178..240] = [t:178..240] ;4)sentence:[e:244..434] = [t:244..434] ;5)sentence:[e:435..634] = [t:435..634] ;6)sentence:[e:635..762] = [t:635..762] ;7)sentence:[e:763..939] = [t:763..939] ;8)sentence:[e:940..1078] = [t:940..1078] ;9)sentence:[e:1079..1322] = [t:1079..1322] ;10)section:[e:1326..1370] = [t:1326..1370] ;section 0 Span:0..32 ;Gene. 1991 Jun 30;102(2):283-8. (SEC (FRAG (NN:[0..4] Gene) (IN:[4..5] .) (CD:[7..11] 1991) (NNP:[12..15] Jun) (CD:[16..18] 30) (CD:[18..23] ;102-LRB-) (CD:[23..24] 2) (-RRB-:[24..25] -RRB-) (::[25..26] :) (CD:[26..29] 283) (::[29..30] -) (CD:[30..32] 8.))) ;sentence 1 Span:38..146 ;Isolation and analysis of a human cDNA highly homologous to the yeast gene ;encoding L17A ribosomal protein. ;[123..127]:gene-protein:"L17A" (SENT (NP-HLN (NP (NN:[38..47] Isolation) (CC:[48..51] and) (NN:[52..60] analysis)) (PP (IN:[61..63] of) (NP (NP (DT:[64..65] a) (JJ:[66..71] human) (NN:[72..76] cDNA)) (ADJP (RB:[77..83] highly) (JJ:[84..94] homologous) (PP (TO:[95..97] to) (NP (NP (DT:[98..101] the) (NN:[102..107] yeast) (NN:[108..112] gene)) (VP (VBG:[114..122] encoding) (NP (NN:[123..127] L17A) (JJ:[128..137] ribosomal) (NN:[138..145] protein)))))))) (.:[145..146] .))) ;section 2 Span:150..174 ;Berchtold MW, Berger MC. (SEC (FRAG (NNP:[150..159] Berchtold) (NNP:[160..162] MW) (,:[162..163] ,) (NNP:[164..170] Berger) (NNP:[171..173] MC) (.:[173..174] .))) ;section 3 Span:178..240 ;Institut fur Pharmakologie und Biochemie, Zurich, Switzerland. (SEC (FRAG (NNP:[178..186] Institut) (NN:[187..190] fur) (NNP:[191..204] Pharmakologie) (VBD:[205..208] und) (NNP:[209..218] Biochemie) (,:[218..219] ,) (NNP:[220..226] Zurich) (,:[226..227] ,) (NNP:[228..239] Switzerland) (.:[239..240] .))) ;sentence 4 Span:244..434 ;A cDNA from human brain poly(A)+RNA with significant similarity to the gene ;encoding yeast L17A large subunit ribosomal (r) protein (L17A) was isolated ;using the polymerase chain reaction. ;[336..376]:gene-protein:"L17A large subunit ribosomal (r) protein" ;[378..382]:gene-protein:"L17A" ;[408..418]:gene-protein:"polymerase" (SENT (S (NP-SBJ-1 (NP (DT:[244..245] A) (NN:[246..250] cDNA)) (PP (IN:[251..255] from) (NP (JJ:[256..261] human) (NN:[262..267] brain) (NN:[268..275] poly-LRB-A-RRB-) (SYM:[275..276] +) (NN:[276..279] RNA))) (PP (IN:[280..284] with) (NP (NP (JJ:[285..296] significant) (NN:[297..307] similarity)) (PP (TO:[308..310] to) (NP (NP (DT:[311..314] the) (NN:[315..319] gene)) (VP (VBG:[321..329] encoding) (NP (NN:[330..335] yeast) (NN:[336..340] L17A) (NML (JJ:[341..346] large) (NN:[347..354] subunit) (JJ:[355..364] ribosomal) (PRN (-LRB-:[365..366] -LRB-) (NP (JJ:[366..367] r)) (-RRB-:[367..368] -RRB-)) (NN:[369..376] protein)) (PRN (-LRB-:[377..378] -LRB-) (NP (NN:[378..382] L17A)) (-RRB-:[382..383] -RRB-))))))))) (VP (VBD:[384..387] was) (VP (VBN:[388..396] isolated) (NP-1 (-NONE-:[396..396] *)) (S-MNR (NP-SBJ (-NONE-:[396..396] *)) (VP (VBG:[398..403] using) (NP (DT:[404..407] the) (NN:[408..418] polymerase) (NN:[419..424] chain) (NN:[425..433] reaction)))))) (.:[433..434] .))) ;sentence 5 Span:435..634 ;The deduced amino acid (aa) sequence of 140 aa (calculated pI of 10.79) ;exhibits a 78% similarity to that of the yeast L17A r protein (88% when ;conservative aa replacements are considered as well). ;[555..570]:gene-protein:"L17A r protein" (SENT (S (NP-SBJ (NP (DT:[435..438] The) (VBN:[439..446] deduced) (NML (JJ:[447..452] amino) (NN:[453..457] acid)) (NML (-LRB-:[458..459] -LRB-) (NN:[459..461] aa) (-RRB-:[461..462] -RRB-)) (NN:[463..471] sequence)) (PP (IN:[472..474] of) (NP (CD:[475..478] 140) (NN:[480..482] aa) (PRN (-LRB-:[483..484] -LRB-) (NP (VBN:[484..494] calculated) (NN:[495..497] pI)) (PP (IN:[498..500] of) (NP (CD:[501..506] 10.79))) (-RRB-:[506..507] -RRB-))))) (VP (VBZ:[508..516] exhibits) (NP (NP (DT:[517..518] a) (NML (CD:[519..521] 78) (NN:[521..522] %)) (NN:[523..533] similarity)) (PP (TO:[534..536] to) (NP (NP (DT:[537..541] that)) (PP (IN:[542..544] of) (NP (DT:[545..548] the) (NN:[549..554] yeast) (NN:[555..559] L17A) (JJ:[561..562] r) (NN:[563..570] protein)))))) (PRN (-LRB-:[571..572] -LRB-) (FRAG (NP (CD:[572..574] 88) (NN:[574..575] %)) (SBAR (WHADVP-2 (WRB:[576..580] when)) (S (NP-SBJ-1 (JJ:[581..593] conservative) (NN:[594..596] aa) (NNS:[597..609] replacements)) (VP (VBP:[610..613] are) (VP (VBN:[614..624] considered) (NP-1 (-NONE-:[624..624] *)) (ADVP (RB:[625..627] as) (RB:[628..632] well)) (ADVP-2 (-NONE-:[632..632] *T*))))))) (-RRB-:[632..633] -RRB-))) (.:[633..634] .))) ;sentence 6 Span:635..762 ;This indicates that L17A is one of the best conserved r-proteins and ;therefore may play a critical role in ribosome function. ;[656..660]:gene-protein:"L17A" ;[690..700]:gene-protein:"r-proteins" (SENT (S (NP-SBJ (DT:[635..639] This)) (VP (VBZ:[641..650] indicates) (SBAR (IN:[651..655] that) (S (NP-SBJ (NN:[656..660] L17A)) (VP (VP (VBZ:[661..663] is) (NP-PRD (NP (CD:[664..667] one)) (PP (IN:[668..670] of) (NP (DT:[671..674] the) (ADJP (RBS:[675..679] best) (VBN:[680..689] conserved)) (JJ:[690..691] r) (HYPH:[691..692] -) (NNS:[692..700] proteins))))) (CC:[701..704] and) (VP (ADVP (RB:[705..714] therefore)) (MD:[715..718] may) (VP (VB:[720..724] play) (NP (DT:[725..726] a) (JJ:[727..735] critical) (NN:[736..740] role)) (PP (IN:[741..743] in) (NP (NN:[744..752] ribosome) (NN:[753..761] function))))))))) (.:[761..762] .))) ;sentence 7 Span:763..939 ;In contrast to its eubacterial and chloroplast counterparts, human L17A ;contains an N-terminal extension of 19 aa which may be involved in nuclear ;targeting of the r-protein. ;[831..835]:gene-protein:"L17A" ;[929..938]:gene-protein:"r-protein" (SENT (S (PP (IN:[763..765] In) (NP (NP (NN:[766..774] contrast)) (PP (TO:[775..777] to) (NP (PRP$:[778..781] its) (NML (NML (JJ:[782..793] eubacterial) (NML-2 (-NONE-:[793..793] *P*))) (CC:[794..797] and) (NML (NN:[799..810] chloroplast) (NML-2 (NNS:[811..823] counterparts)))))))) (,:[823..824] ,) (NP-SBJ (JJ:[825..830] human) (NN:[831..835] L17A)) (VP (VBZ:[836..844] contains) (NP (NP (NP (DT:[845..847] an) (NML (NN:[848..849] N) (HYPH:[849..850] -) (JJ:[850..858] terminal)) (NN:[859..868] extension)) (PP (IN:[869..871] of) (NP (CD:[872..874] 19) (NN:[875..877] aa)))) (SBAR (WHNP-1 (WDT:[879..884] which)) (S (NP-SBJ-1 (-NONE-:[884..884] *T*)) (VP (MD:[885..888] may) (VP (VB:[889..891] be) (VP (VBN:[892..900] involved) (NP-1 (-NONE-:[900..900] *)) (PP-CLR (IN:[901..903] in) (NP (NP (JJ:[904..911] nuclear) (NN:[912..921] targeting)) (PP (IN:[922..924] of) (NP (DT:[925..928] the) (JJ:[929..930] r) (HYPH:[930..931] -) (NN:[931..938] protein)))))))))))) (.:[938..939] .))) ;sentence 8 Span:940..1078 ;Approximately five to seven genes in mammalian genomes give strong ;hybridization signals when probed with the human L17A homologue cDNA. ;[1058..1062]:gene-rna:"L17A" (SENT (S (NP-SBJ (NP (QP (RB:[940..953] Approximately) (CD:[954..958] five) (TO:[960..962] to) (CD:[963..968] seven)) (NNS:[969..974] genes)) (PP (IN:[975..977] in) (NP (JJ:[978..987] mammalian) (NNS:[988..995] genomes)))) (VP (VBP:[996..1000] give) (NP (JJ:[1001..1007] strong) (NN:[1008..1021] hybridization) (NNS:[1022..1029] signals)) (SBAR-ADV (WHADVP-2 (WRB:[1030..1034] when)) (S (NP-SBJ-1 (-NONE-:[1034..1034] *)) (VP (VBN:[1036..1042] probed) (NP-1 (-NONE-:[1042..1042] *)) (PP (IN:[1043..1047] with) (NP (DT:[1048..1051] the) (JJ:[1052..1057] human) (NN:[1058..1062] L17A) (NN:[1063..1072] homologue) (NN:[1073..1077] cDNA))) (ADVP-TMP-2 (-NONE-:[1077..1077] *T*)))))) (.:[1077..1078] .))) ;sentence 9 Span:1079..1322 ;Whereas the L17A homologue was found to be expressed at similar levels in ;several human tissues as a transcript of 600 nucleotides, a several-fold ;higher transcript level was detected in the rapidly growing neuroblastoma ;cell line, SK-N-BE. ;[1091..1095]:gene-rna:"L17A" ;[1289..1302]:malignancy-type:"neuroblastoma" (SENT (S (SBAR-ADV (IN:[1079..1086] Whereas) (S (NP-SBJ-1 (DT:[1087..1090] the) (NN:[1091..1095] L17A) (NN:[1096..1105] homologue)) (VP (VBD:[1106..1109] was) (VP (VBN:[1110..1115] found) (S (NP-SBJ-1 (-NONE-:[1115..1115] *)) (VP (TO:[1117..1119] to) (VP (VB:[1120..1122] be) (VP (VBN:[1123..1132] expressed) (NP-1 (-NONE-:[1132..1132] *)) (PP (IN:[1133..1135] at) (NP (NP (JJ:[1136..1143] similar) (NNS:[1144..1150] levels)) (PP-3 (-NONE-:[1150..1150] *ICH*)))) (PP-LOC (IN:[1151..1153] in) (NP (JJ:[1154..1161] several) (JJ:[1162..1167] human) (NNS:[1168..1175] tissues))) (PP-3 (IN:[1176..1178] as) (NP (NP (DT:[1179..1180] a) (NN:[1181..1191] transcript)) (PP (IN:[1192..1194] of) (NP (CD:[1196..1199] 600) (NNS:[1200..1211] nucleotides))))))))))))) (,:[1211..1212] ,) (NP-SBJ-2 (DT:[1213..1214] a) (ADJP (QP (JJ:[1215..1222] several) (HYPH:[1222..1223] -) (RB:[1223..1227] fold)) (JJR:[1228..1234] higher)) (NN:[1235..1245] transcript) (NN:[1246..1251] level)) (VP (VBD:[1252..1255] was) (VP (VBN:[1256..1264] detected) (NP-2 (-NONE-:[1264..1264] *)) (PP (IN:[1265..1267] in) (NP (NP (DT:[1268..1271] the) (ADJP (RB:[1273..1280] rapidly) (VBG:[1281..1288] growing)) (NN:[1289..1302] neuroblastoma) (NN:[1303..1307] cell) (NN:[1308..1312] line)) (,:[1312..1313] ,) (NP (NN:[1314..1321] SK-N-BE)))))) (.:[1321..1322] .))) ;section 10 Span:1326..1370 ;PMID: 1874450 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1326..1330] PMID) (::[1330..1331] :) (CD:[1332..1339] 1874450) (NN:[1340..1341] -LSB-) (NNP:[1341..1347] PubMed) (::[1348..1349] -) (NN:[1350..1357] indexed) (IN:[1358..1361] for) (NNP:[1362..1370] MEDLINE-RSB-)))