;PMID: 8858760 ;source_file_2856.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..42] = [t:0..42] ;1)sentence:[e:48..125] = [t:48..125] ;2)section:[e:129..157] = [t:129..157] ;3)section:[e:161..278] = [t:161..278] ;4)sentence:[e:282..501] = [t:282..501] ;5)sentence:[e:502..663] = [t:502..663] ;6)sentence:[e:664..818] = [t:664..818] ;7)sentence:[e:819..908] = [t:819..908] ;8)sentence:[e:909..1020] = [t:909..1020] ;9)sentence:[e:1021..1147] = [t:1021..1147] ;10)sentence:[e:1148..1253] = [t:1148..1253] ;11)section:[e:1257..1275] = [t:1257..1275] ;12)section:[e:1281..1287] = [t:1281..1287] ;13)section:[e:1293..1309] = [t:1293..1309] ;14)section:[e:1313..1357] = [t:1313..1357] ;section 0 Span:0..42 ;Semin Pediatr Surg. 1996 Aug;5(3):149-54. (SEC (FRAG (NNP:[0..5] Semin) (NNP:[6..13] Pediatr) (NNP:[14..18] Surg) (.:[18..19] .) (CD:[21..25] 1996) (IN:[26..30] Aug;) (CD:[30..31] 5) (NN:[31..32] -LRB-) (NN:[32..33] 3) (-RRB-:[33..34] -RRB-) (::[34..35] :) (CD:[35..39] 149-) (CD:[39..41] 54) (.:[41..42] .))) ;sentence 1 Span:48..125 ;cDNA subtraction hybridization: a review and an application to ;neuroblastoma. ;[111..124]:malignancy-type:"neuroblastoma" (SENT (NP-HLN (NP (NN:[48..52] cDNA) (NN:[53..64] subtraction) (NN:[65..78] hybridization)) (::[78..79] :) (NP (NP (DT:[80..81] a) (NN:[82..88] review)) (CC:[89..92] and) (NP (NP (DT:[93..95] an) (NN:[96..107] application)) (PP (TO:[108..110] to) (NP (NN:[111..124] neuroblastoma))))) (.:[124..125] .))) ;section 2 Span:129..157 ;Swendeman SL, La Quaglia MP. (SEC (FRAG (NNP:[129..138] Swendeman) (NNP:[139..141] SL) (,:[141..142] ,) (NNP:[143..145] La) (NNP:[146..153] Quaglia) (NN:[154..156] MP) (.:[156..157] .))) ;section 3 Span:161..278 ;Department of Surgery (Pediatric Surgical Service), Memorial Sloan-Kettering ;Cancer Center, New York, NY 10021, USA. (SEC (FRAG (NNP:[161..171] Department) (IN:[172..174] of) (NNP:[175..182] Surgery) (-LRB-:[183..184] -LRB-) (NNP:[184..193] Pediatric) (NNP:[194..202] Surgical) (NNP:[203..210] Service) (-RRB-:[210..211] -RRB-) (,:[211..212] ,) (JJ:[213..221] Memorial) (NNP:[222..227] Sloan) (HYPH:[227..228] -) (NNP:[228..237] Kettering) (NNP:[239..245] Cancer) (NNP:[246..252] Center) (,:[252..253] ,) (NNP:[254..257] New) (NNP:[258..262] York) (,:[262..263] ,) (NNP:[264..266] NY) (CD:[267..272] 10021) (,:[272..273] ,) (NNP:[274..277] USA) (.:[277..278] .))) ;sentence 4 Span:282..501 ;Recent advances in molecular biological techniques have increased our ;capability to distinguish the small differences in gene expression between ;subpopulations of cells found within specific tissues or tumor isolates. (SENT (S (NP-SBJ (NP (JJ:[282..288] Recent) (NNS:[289..297] advances)) (PP (IN:[298..300] in) (NP (JJ:[301..310] molecular) (JJ:[311..321] biological) (NNS:[322..332] techniques)))) (VP (VBP:[333..337] have) (VP (VBN:[338..347] increased) (NP (PRP$:[348..351] our) (NN:[352..362] capability) (S (NP-SBJ (-NONE-:[362..362] *)) (VP (TO:[364..366] to) (VP (VB:[367..378] distinguish) (NP (NP (DT:[379..382] the) (JJ:[383..388] small) (NNS:[389..400] differences)) (PP (IN:[401..403] in) (NP (NN:[404..408] gene) (NN:[409..419] expression)))) (PP (IN:[420..427] between) (NP (NP (NP (NNS:[428..442] subpopulations)) (PP (IN:[444..446] of) (NP (NP (NNS:[447..452] cells)) (VP (VBN:[453..458] found) (NP (-NONE-:[458..458] *)) (PP-LOC (IN:[459..465] within) (NP (JJ:[466..474] specific) (NNS:[475..482] tissues))))))) (CC:[483..485] or) (NP (NN:[486..491] tumor) (NNS:[492..500] isolates)))))))))) (.:[500..501] .))) ;sentence 5 Span:502..663 ;We use subtractive hybridization or subtractive cloning to generate ;information regarding genes that direct various aspects of mammalian ;embryonic development. (SENT (S (NP-SBJ (PRP:[502..504] We)) (VP (VBP:[505..508] use) (NP (NP (JJ:[509..520] subtractive) (NN:[522..535] hybridization)) (CC:[536..538] or) (NP (JJ:[539..550] subtractive) (NN:[551..558] cloning))) (S-PRP (NP-SBJ (-NONE-:[558..558] *)) (VP (TO:[559..561] to) (VP (VB:[562..570] generate) (NP (NP (NN:[571..582] information)) (PP (IN:[583..592] regarding) (NP (NP (NNS:[593..598] genes)) (SBAR (WHNP-1 (WDT:[600..604] that)) (S (NP-SBJ-1 (-NONE-:[604..604] *T*)) (VP (VBP:[605..611] direct) (NP (NP (JJ:[612..619] various) (NNS:[620..627] aspects)) (PP (IN:[628..630] of) (NP (JJ:[631..640] mammalian) (JJ:[641..650] embryonic) (NN:[651..662] development)))))))))))))) (.:[662..663] .))) ;sentence 6 Span:664..818 ;The technique also is used to identify genes with specificity for particular ;tissues or cell types or those that regulate various processes in the cell. (SENT (S (NP-SBJ-1 (DT:[664..667] The) (NN:[668..677] technique)) (ADVP (RB:[679..683] also)) (VP (VBZ:[684..686] is) (VP (VBN:[687..691] used) (NP-1 (-NONE-:[691..691] *)) (S-ADV (NP-SBJ (-NONE-:[691..691] *)) (VP (TO:[692..694] to) (VP (VB:[695..703] identify) (NP (NP (NNS:[704..709] genes)) (PP (IN:[710..714] with) (NP (NP (NP (NN:[715..726] specificity)) (PP (IN:[727..730] for) (NP (NP (JJ:[731..741] particular) (NNS:[742..749] tissues)) (CC:[750..752] or) (NP (NN:[753..757] cell) (NNS:[759..764] types))))) (CC:[765..767] or) (NP (NP (DT:[768..773] those)) (SBAR (WHNP-2 (WDT:[774..778] that)) (S (NP-SBJ-2 (-NONE-:[778..778] *T*)) (VP (VBP:[779..787] regulate) (NP (JJ:[788..795] various) (NNS:[796..805] processes)) (PP-LOC (IN:[806..808] in) (NP (DT:[809..812] the) (NN:[813..817] cell))))))))))))))) (.:[817..818] .))) ;sentence 7 Span:819..908 ;Also, subject to analysis is the aberrant expression of genes involved in ;tumorigenesis. (SENT (SINV (ADVP (RB:[819..823] Also)) (,:[823..824] ,) (ADJP-PRD-TPC-1 (JJ:[825..832] subject) (PP (TO:[833..835] to) (NP (NN:[837..845] analysis)))) (VP (VBZ:[846..848] is) (ADJP-PRD-1 (-NONE-:[848..848] *T*))) (NP-SBJ (NP (DT:[849..852] the) (JJ:[853..861] aberrant) (NN:[862..872] expression)) (PP (IN:[873..875] of) (NP (NP (NNS:[876..881] genes)) (VP (VBN:[882..890] involved) (NP (-NONE-:[890..890] *)) (PP-CLR (IN:[891..893] in) (NP (NN:[894..907] tumorigenesis))))))) (.:[907..908] .))) ;sentence 8 Span:909..1020 ;Another use is analysis of subpopulations of cell types previously ;identified within individual solid tumors. (SENT (S (NP-SBJ (DT:[909..916] Another) (NN:[918..921] use)) (VP (VBZ:[922..924] is) (NP (NP (NP (NN:[925..933] analysis)) (PP (IN:[934..936] of) (NP (NP (NNS:[937..951] subpopulations)) (PP (IN:[952..954] of) (NP (NN:[955..959] cell) (NNS:[960..965] types)))))) (VP (ADVP-TMP (RB:[966..976] previously)) (VBN:[977..987] identified) (NP (-NONE-:[987..987] *)) (PP-LOC (IN:[988..994] within) (NP (JJ:[996..1006] individual) (JJ:[1007..1012] solid) (NNS:[1013..1019] tumors)))))) (.:[1019..1020] .))) ;sentence 9 Span:1021..1147 ;We used subtractive cloning in the analysis of cell line subpopulations ;derived from the human pediatric tumor neuroblastoma. ;[1117..1126]:malignancy-developmental-state:"pediatric" ;[1133..1146]:malignancy-type:"neuroblastoma" (SENT (S (NP-SBJ (PRP:[1021..1023] We)) (VP (VBD:[1024..1028] used) (NP (JJ:[1029..1040] subtractive) (NN:[1041..1048] cloning)) (PP (IN:[1049..1051] in) (NP (NP (DT:[1052..1055] the) (NN:[1056..1064] analysis)) (PP (IN:[1065..1067] of) (NP (NP (NP (NN:[1068..1072] cell) (NN:[1074..1078] line)) (NNS:[1079..1093] subpopulations)) (VP (VBN:[1094..1101] derived) (NP (-NONE-:[1101..1101] *)) (PP-CLR (IN:[1102..1106] from) (NP (DT:[1107..1110] the) (JJ:[1111..1116] human) (JJ:[1117..1126] pediatric) (NN:[1127..1132] tumor) (NN:[1133..1146] neuroblastoma))))))))) (.:[1146..1147] .))) ;sentence 10 Span:1148..1253 ;This has resulted in the identification of novel genes that may be useful in ;the study of this disease. (SENT (S (NP-SBJ (DT:[1148..1152] This)) (VP (VBZ:[1154..1157] has) (VP (VBN:[1158..1166] resulted) (PP-CLR (IN:[1167..1169] in) (NP (NP (DT:[1170..1173] the) (NN:[1174..1188] identification)) (PP (IN:[1189..1191] of) (NP (NP (JJ:[1192..1197] novel) (NNS:[1198..1203] genes)) (SBAR (WHNP-1 (WDT:[1204..1208] that)) (S (NP-SBJ-1 (-NONE-:[1208..1208] *T*)) (VP (MD:[1209..1212] may) (VP (VB:[1213..1215] be) (VP (JJ:[1216..1222] useful) (PP (IN:[1223..1225] in) (NP (NP (DT:[1226..1229] the) (NN:[1231..1236] study)) (PP (IN:[1237..1239] of) (NP (DT:[1240..1244] this) (NN:[1245..1252] disease)))))))))))))))) (.:[1252..1253] .))) ;section 11 Span:1257..1275 ;Publication Types: (SEC (FRAG (NNP:[1257..1268] Publication) (NNP:[1269..1274] Types) (::[1274..1275] :))) ;section 12 Span:1281..1287 ;Review (SEC (FRAG (NNP:[1281..1287] Review))) ;section 13 Span:1293..1309 ;Review, Tutorial (SEC (FRAG (NNP:[1293..1299] Review) (,:[1299..1300] ,) (NNP:[1301..1309] Tutorial))) ;section 14 Span:1313..1357 ;PMID: 8858760 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1313..1317] PMID) (::[1317..1318] :) (CD:[1319..1326] 8858760) (NN:[1327..1328] -LSB-) (NNP:[1328..1334] PubMed) (::[1335..1336] -) (NN:[1337..1344] indexed) (IN:[1345..1348] for) (NNP:[1349..1357] MEDLINE-RSB-)))