;PMID: 1059097 ;source_file_2855.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..50] = [t:0..50] ;1)sentence:[e:56..127] = [t:56..127] ;2)section:[e:131..154] = [t:131..154] ;3)sentence:[e:158..283] = [t:158..283] ;4)sentence:[e:284..372] = [t:284..372] ;5)sentence:[e:373..523] = [t:373..523] ;6)sentence:[e:524..625] = [t:524..625] ;7)sentence:[e:627..718] = [t:627..718] ;8)sentence:[e:719..908] = [t:719..908] ;9)sentence:[e:909..1116] = [t:909..1116] ;10)sentence:[e:1117..1309] = [t:1117..1309] ;11)section:[e:1313..1357] = [t:1313..1357] ;section 0 Span:0..50 ;Proc Natl Acad Sci U S A. 1975 Aug;72(8):3119-23. (SEC (FRAG (NNP:[0..4] Proc) (NNP:[5..9] Natl) (NNP:[10..14] Acad) (NNP:[15..18] Sci) (NNP:[19..20] U) (NNP:[21..22] S) (NNP:[23..24] A) (.:[24..25] .) (CD:[27..31] 1975) (CC:[32..39] Aug;72-LRB-) (CD:[39..40] 8) (-RRB-:[40..41] -RRB-) (CD:[41..46] :3119) (::[46..47] -) (CD:[47..49] 23) (.:[49..50] .))) ;sentence 1 Span:56..127 ;Gene expression in mouse neuroblastoma cells: properties of the genome. ;[81..94]:malignancy-type:"neuroblastoma" (SENT (NP-HLN (NP (NP (NN:[56..60] Gene) (NN:[61..71] expression)) (PP-LOC (IN:[72..74] in) (NP (NN:[75..80] mouse) (NN:[81..94] neuroblastoma) (NNS:[95..100] cells)))) (::[100..101] :) (NP (NP (NNS:[102..112] properties)) (PP (IN:[113..115] of) (NP (DT:[116..119] the) (NN:[120..126] genome)))) (.:[126..127] .))) ;section 2 Span:131..154 ;Zornetzer MS, Stein GS. (SEC (FRAG (NNP:[131..140] Zornetzer) (NNP:[141..143] MS) (,:[143..144] ,) (NNP:[145..150] Stein) (NNP:[151..153] GS) (.:[153..154] .))) ;sentence 3 Span:158..283 ;Chromatin was prepared from isolated nuclei of proliferating and ;differentiated cultures of C1300 mouse neuroblastoma cells. ;[263..276]:malignancy-type:"neuroblastoma" (SENT (S (NP-SBJ-1 (NN:[158..167] Chromatin)) (VP (VBD:[168..171] was) (VP (VBN:[172..180] prepared) (NP-1 (-NONE-:[180..180] *)) (PP (IN:[181..185] from) (NP (NP (VBN:[186..194] isolated) (NNS:[195..201] nuclei)) (PP (IN:[202..204] of) (NP (NP (NP (VBG:[205..218] proliferating) (NML-2 (-NONE-:[218..218] *P*))) (CC:[219..222] and) (NP (VBN:[223..237] differentiated) (NML-2 (NNS:[239..247] cultures)))) (PP (IN:[248..250] of) (NP (NN:[251..256] C1300) (NN:[257..262] mouse) (NN:[263..276] neuroblastoma) (NNS:[277..282] cells))))))))) (.:[282..283] .))) ;sentence 4 Span:284..372 ;Differentiation was induced by serum withdrawal or treatment with dibutyryl ;cyclic AMP. (SENT (S (NP-SBJ-1 (NN:[284..299] Differentiation)) (VP (VBD:[300..303] was) (VP (VBN:[304..311] induced) (NP-1 (-NONE-:[311..311] *)) (PP (IN:[312..314] by) (NP-LGS (NP (NN:[316..321] serum) (NN:[322..332] withdrawal)) (CC:[333..335] or) (NP (NP (NN:[336..345] treatment)) (PP (IN:[346..350] with) (NP (NN:[351..360] dibutyryl) (JJ:[361..367] cyclic) (NN:[368..371] AMP)))))))) (.:[371..372] .))) ;sentence 5 Span:373..523 ;The ability to support DNA-dependent RNA synthesis when assayed in a ;cell-free system is three times greater for chromatin from proliferating ;cells. (SENT (S (NP-SBJ (DT:[373..376] The) (NN:[377..384] ability) (S (NP-SBJ (-NONE-:[384..384] *)) (VP (TO:[385..387] to) (VP (VB:[388..395] support) (NP (ADJP (NN:[397..400] DNA) (HYPH:[400..401] -) (JJ:[401..410] dependent)) (NN:[411..414] RNA) (NN:[415..424] synthesis)) (SBAR-ADV (WHADVP-1 (WRB:[425..429] when)) (S (NP-SBJ-2 (-NONE-:[429..429] *)) (VP (VBN:[430..437] assayed) (NP-2 (-NONE-:[437..437] *)) (PP (IN:[438..440] in) (NP (DT:[441..442] a) (ADJP (NN:[443..447] cell) (HYPH:[447..448] -) (JJ:[448..452] free)) (NN:[453..459] system))) (ADVP-TMP-1 (-NONE-:[459..459] *T*))))))))) (VP (VBZ:[460..462] is) (ADJP-PRD (ADJP (NML (CD:[463..468] three) (NNS:[469..474] times)) (JJR:[476..483] greater)) (PP (IN:[484..487] for) (NP (NP (NN:[488..497] chromatin)) (PP (IN:[498..502] from) (NP (VBG:[503..516] proliferating) (NNS:[517..522] cells))))))) (.:[522..523] .))) ;sentence 6 Span:524..625 ;Histones isolated from proliferating and differentiated cells were ;fractionated electrophoretically. ;[524..532]:gene-protein:"Histones" (SENT (S (NP-SBJ-2 (NP (NNS:[524..532] Histones)) (VP (VBN:[533..541] isolated) (NP (-NONE-:[541..541] *)) (PP (IN:[542..546] from) (NP (NP (VBG:[548..561] proliferating) (NML-1 (-NONE-:[561..561] *P*))) (CC:[562..565] and) (NP (VBN:[566..580] differentiated) (NML-1 (NNS:[581..586] cells))))))) (VP (VBD:[587..591] were) (VP (VBN:[592..604] fractionated) (NP-2 (-NONE-:[604..604] *)) (ADVP-MNR (RB:[605..624] electrophoretically)))) (.:[624..625] .))) ;sentence 7 Span:627..718 ;The relative amounts of proteins present in the five major histone fractions ;were similar. ;[686..693]:gene-protein:"histone" (SENT (S (NP-SBJ (NP (DT:[627..630] The) (JJ:[631..639] relative) (NNS:[640..647] amounts)) (PP (IN:[648..650] of) (NP (NP (NNS:[651..659] proteins)) (ADJP (JJ:[660..667] present) (PP (IN:[668..670] in) (NP (DT:[671..674] the) (CD:[675..679] five) (JJ:[680..685] major) (NN:[686..693] histone) (NNS:[694..703] fractions))))))) (VP (VBD:[705..709] were) (ADJP-PRD (JJ:[710..717] similar))) (.:[717..718] .))) ;sentence 8 Span:719..908 ;In contrast, there were significant differences in the nonhistone ;chromosomal proteins synthesized and associated with the genome of ;proliferating and differentiating neuroblastoma cells. ;[888..901]:malignancy-type:"neuroblastoma" (SENT (S (PP (IN:[719..721] In) (NP (NN:[722..730] contrast))) (,:[730..731] ,) (NP-SBJ (EX:[732..737] there)) (VP (VBD:[738..742] were) (NP-PRD (NP (JJ:[743..754] significant) (NNS:[755..766] differences)) (PP (IN:[767..769] in) (NP (NP (DT:[770..773] the) (JJ:[774..784] nonhistone) (JJ:[786..797] chromosomal) (NNS:[798..806] proteins)) (VP (VBN:[807..818] synthesized) (CC:[819..822] and) (VBN:[823..833] associated) (NP (-NONE-:[833..833] *)) (PP-CLR (IN:[834..838] with) (NP (NP (DT:[839..842] the) (NN:[843..849] genome)) (PP (IN:[850..852] of) (NP (NP (VBG:[853..866] proliferating) (NML-1 (-NONE-:[866..866] *P*))) (CC:[868..871] and) (NP (VBG:[872..887] differentiating) (NML-1 (NN:[888..901] neuroblastoma) (NNS:[902..907] cells)))))))))))) (.:[907..908] .))) ;sentence 9 Span:909..1116 ;Such differences are reflected by modifications in the electrophoretic ;banding patterns and in incorporation of [3H]tryptophan into various ;molecular weight classes of nonhistone chromosomal polypeptides. (SENT (S (NP-SBJ-1 (JJ:[909..913] Such) (NNS:[914..925] differences)) (VP (VBP:[926..929] are) (VP (VBN:[930..939] reflected) (NP-1 (-NONE-:[939..939] *)) (PP (IN:[940..942] by) (NP (NP (NNS:[944..957] modifications)) (PP (PP (IN:[958..960] in) (NP (DT:[961..964] the) (JJ:[965..980] electrophoretic) (NN:[981..988] banding) (NNS:[989..997] patterns))) (CC:[998..1001] and) (PP (IN:[1002..1004] in) (NP (NP (NN:[1005..1018] incorporation)) (PP (IN:[1019..1021] of) (NP (NN:[1023..1037] -LSB-3H-RSB-tryptophan))) (PP (IN:[1038..1042] into) (NP (NP (JJ:[1043..1050] various) (NML (JJ:[1051..1060] molecular) (NN:[1061..1067] weight)) (NNS:[1068..1075] classes)) (PP (IN:[1076..1078] of) (NP (JJ:[1079..1089] nonhistone) (JJ:[1090..1101] chromosomal) (NNS:[1103..1115] polypeptides)))))))))))) (.:[1115..1116] .))) ;sentence 10 Span:1117..1309 ;A functional relationship between changes in the nonhistone chromosomal ;proteins and variations in the transcriptional activity accompanying ;differentiation of neuroblastoma cells may exist. ;[1279..1292]:malignancy-type:"neuroblastoma" (SENT (S (NP-SBJ (NP (DT:[1117..1118] A) (JJ:[1119..1129] functional) (NN:[1130..1142] relationship)) (PP (IN:[1143..1150] between) (NP (NP (NP (NNS:[1151..1158] changes)) (PP (IN:[1159..1161] in) (NP (DT:[1162..1165] the) (JJ:[1166..1176] nonhistone) (JJ:[1178..1189] chromosomal) (NNS:[1190..1198] proteins)))) (CC:[1199..1202] and) (NP (NP (NNS:[1203..1213] variations)) (PP (IN:[1214..1216] in) (NP (NP (DT:[1217..1220] the) (JJ:[1221..1236] transcriptional) (NN:[1237..1245] activity)) (VP (VBG:[1246..1258] accompanying) (NP (NP (NN:[1260..1275] differentiation)) (PP (IN:[1276..1278] of) (NP (NN:[1279..1292] neuroblastoma) (NNS:[1293..1298] cells))))))))))) (VP (MD:[1299..1302] may) (VP (VB:[1303..1308] exist))) (.:[1308..1309] .))) ;section 11 Span:1313..1357 ;PMID: 1059097 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1313..1317] PMID) (::[1317..1318] :) (CD:[1319..1326] 1059097) (NN:[1327..1328] -LSB-) (NNP:[1328..1334] PubMed) (::[1335..1336] -) (NN:[1337..1344] indexed) (IN:[1345..1348] for) (NNP:[1349..1357] MEDLINE-RSB-)))