;PMID: 10466827 ;source_file_2850.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..42] = [t:0..42] ;1)sentence:[e:48..155] = [t:48..155] ;2)section:[e:159..222] = [t:159..222] ;3)section:[e:226..329] = [t:226..329] ;4)sentence:[e:333..554] = [t:333..554] ;5)sentence:[e:555..804] = [t:555..804] ;6)sentence:[e:805..1043] = [t:805..1043] ;7)sentence:[e:1044..1296] = [t:1044..1296] ;8)section:[e:1300..1345] = [t:1300..1345] ;section 0 Span:0..42 ;J Gen Virol. 1999 Aug;80 ( Pt 8):2275-83. (SEC (FRAG (NNP:[0..1] J) (NNP:[2..5] Gen) (NNP:[6..11] Virol) (.:[11..12] .) (CD:[14..18] 1999) (.:[19..25] Aug;80) (-LRB-:[26..27] -LRB-) (NN:[28..30] Pt) (CD:[31..32] 8) (-RRB-:[32..33] -RRB-) (CD:[33..38] :2275) (CD:[38..41] -83) (.:[41..42] .))) ;sentence 1 Span:48..155 ;PrP (prion) gene expression in sheep may be modulated by alternative ;polyadenylation of its messenger RNA. ;[48..51]:gene-rna:"PrP" ;[53..58]:gene-rna:"prion" (SENT (S-HLN (NP-SBJ-1 (NP (NML (NML (NN:[48..51] PrP)) (NML (-LRB-:[52..53] -LRB-) (NN:[53..58] prion) (-RRB-:[58..59] -RRB-))) (NN:[60..64] gene) (NN:[65..75] expression)) (PP-LOC (IN:[76..78] in) (NP (NNS:[79..84] sheep)))) (VP (MD:[85..88] may) (VP (VB:[89..91] be) (VP (VBN:[92..101] modulated) (NP-1 (-NONE-:[101..101] *)) (PP (IN:[102..104] by) (NP-LGS (NP (JJ:[105..116] alternative) (NN:[118..133] polyadenylation)) (PP (IN:[134..136] of) (NP (PRP$:[137..140] its) (NN:[141..150] messenger) (NN:[151..154] RNA)))))))) (.:[154..155] .))) ;section 2 Span:159..222 ;Goldmann W, O'Neill G, Cheung F, Charleson F, Ford P, Hunter N. (SEC (FRAG (NNP:[159..167] Goldmann) (NNP:[168..169] W) (,:[169..170] ,) (NNP:[171..178] O'Neill) (NNP:[179..180] G) (,:[180..181] ,) (NNP:[182..188] Cheung) (NNP:[189..190] F) (,:[190..191] ,) (NNP:[192..201] Charleson) (NNP:[202..203] F) (,:[203..204] ,) (NNP:[205..209] Ford) (NNP:[210..212] P,) (NNP:[213..219] Hunter) (NNP:[220..221] N) (.:[221..222] .))) ;section 3 Span:226..329 ;Neuropathogenesis Unit, BBSRC Institute for Animal Health, Edinburgh, UK. ;Wilfred.Goldmann@bbsrc.ac.uk (SEC (FRAG (NNP:[226..243] Neuropathogenesis) (NNP:[244..248] Unit) (,:[248..249] ,) (NNP:[250..255] BBSRC) (NNP:[256..265] Institute) (IN:[266..269] for) (NNP:[270..276] Animal) (NNP:[277..283] Health) (,:[283..284] ,) (NNP:[285..294] Edinburgh) (,:[294..295] ,) (NNP:[296..299] UK.) (NNP:[301..308] Wilfred) (.:[308..329] .Goldmann@bbsrc.ac.uk))) ;sentence 4 Span:333..554 ;Scrapie-associated fibrils and their major protein component, PrP or prion ;protein, accumulate in the brains and some other tissues of all species ;affected by transmissible spongiform encephalopathies or prion diseases. ;[395..398]:gene-protein:"PrP" ;[402..407]:gene-protein:"prion" ;[539..544]:gene-protein:"prion" (SENT (S (NP-SBJ (NP (ADJP (NNP:[333..340] Scrapie) (HYPH:[340..341] -) (VBN:[341..351] associated)) (NNS:[352..359] fibrils)) (CC:[360..363] and) (NP (NP (PRP$:[364..369] their) (JJ:[370..375] major) (NN:[376..383] protein) (NN:[384..393] component)) (,:[393..394] ,) (NP (NP (NN:[395..398] PrP)) (CC:[399..401] or) (NP (NN:[402..407] prion) (NN:[409..416] protein))))) (,:[416..417] ,) (VP (VBP:[418..428] accumulate) (PP-LOC (IN:[429..431] in) (NP (NP (NP (DT:[432..435] the) (NNS:[436..442] brains)) (CC:[443..446] and) (NP (DT:[447..451] some) (JJ:[452..457] other) (NNS:[458..465] tissues))) (PP (IN:[466..468] of) (NP (NP (DT:[469..472] all) (NNS:[473..480] species)) (VP (VBN:[481..489] affected) (NP (-NONE-:[489..489] *)) (PP (IN:[491..493] by) (NP-LGS (NP (JJ:[494..507] transmissible) (JJ:[508..518] spongiform) (NNS:[519..535] encephalopathies)) (CC:[536..538] or) (NP (NN:[539..544] prion) (NNS:[545..553] diseases)))))))))) (.:[553..554] .))) ;sentence 5 Span:555..804 ;To investigate the role of PrP gene expression in the hosts of these ;diseases, we have analysed some characteristics of PrP gene RNA transcripts ;in sheep and cattle tissues and made comparisons with PrP RNA transcripts in ;human and mouse tissues. ;[583..586]:gene-rna:"PrP" ;[677..680]:gene-rna:"PrP" ;[757..760]:gene-rna:"PrP" (SENT (S (S-PRP (NP-SBJ (-NONE-:[555..555] *)) (VP (TO:[555..557] To) (VP (VB:[558..569] investigate) (NP (NP (DT:[571..574] the) (NN:[575..579] role)) (PP (IN:[580..582] of) (NP (NN:[583..586] PrP) (NN:[587..591] gene) (NN:[592..602] expression))) (PP (IN:[603..605] in) (NP (NP (DT:[606..609] the) (NNS:[610..615] hosts)) (PP (IN:[616..618] of) (NP (DT:[619..624] these) (NNS:[625..633] diseases))))))))) (,:[633..634] ,) (NP-SBJ (PRP:[635..637] we)) (VP (VBP:[638..642] have) (VP (VP (VBN:[643..651] analysed) (NP (NP (DT:[653..657] some) (NNS:[658..673] characteristics)) (PP (IN:[674..676] of) (NP (NP (NN:[677..680] PrP) (NN:[681..685] gene) (NN:[686..689] RNA) (NNS:[690..701] transcripts)) (PP (IN:[702..704] in) (NP (NP (NN:[705..710] sheep) (NML-1 (-NONE-:[710..710] *P*))) (CC:[711..714] and) (NP (NN:[715..721] cattle) (NML-1 (NNS:[722..729] tissues))))))))) (CC:[730..733] and) (VP (VBN:[735..739] made) (NP (NNS:[740..751] comparisons)) (PP (IN:[752..756] with) (NP (NP (NN:[757..760] PrP) (NN:[761..764] RNA) (NNS:[765..776] transcripts)) (PP (IN:[777..779] in) (NP (NP (JJ:[780..785] human) (NML-2 (-NONE-:[785..785] *P*))) (CC:[786..789] and) (NP (NN:[790..795] mouse) (NML-2 (NNS:[796..803] tissues)))))))))) (.:[803..804] .))) ;sentence 6 Span:805..1043 ;Two PrP messenger RNAs of 4.6 kb and 2.1 kb, the result of alternative ;polyadenylation, were found first in sheep peripheral tissues and also ;occurred at low levels in sheep brain and bovine tissues, but not in human ;and mouse tissues. ;[809..812]:gene-rna:"PrP" (SENT (S (NP-SBJ-1 (NP (NP (CD:[805..808] Two) (NN:[809..812] PrP) (NN:[814..823] messenger) (NNS:[824..828] RNAs)) (PP (IN:[829..831] of) (NP (NP (CD:[832..835] 4.6) (NN:[836..838] kb)) (CC:[839..842] and) (NP (CD:[843..846] 2.1) (NN:[847..849] kb))))) (,:[849..850] ,) (NP (NP (DT:[851..854] the) (NN:[855..861] result)) (PP (IN:[862..864] of) (NP (JJ:[865..876] alternative) (NN:[877..892] polyadenylation))))) (,:[892..893] ,) (VP (VBD:[895..899] were) (VP (VP (VBN:[900..905] found) (NP-1 (-NONE-:[905..905] *)) (ADVP (RB:[906..911] first)) (PP-LOC (IN:[912..914] in) (NP (NN:[915..920] sheep) (JJ:[921..931] peripheral) (NNS:[932..939] tissues)))) (CC:[940..943] and) (VP (VP (ADVP (RB:[944..948] also)) (VBD:[949..957] occurred) (PP (IN:[958..960] at) (NP (JJ:[961..964] low) (NNS:[965..971] levels))) (PP=2 (IN:[972..974] in) (NP (NP (NN:[976..981] sheep) (NN:[982..987] brain)) (CC:[988..991] and) (NP (JJ:[992..998] bovine) (NNS:[999..1006] tissues))))) (,:[1006..1007] ,) (CC:[1008..1011] but) (VP (RB:[1012..1015] not) (PP=2 (IN:[1016..1018] in) (NP (NP (JJ:[1019..1024] human) (NML-3 (-NONE-:[1024..1024] *P*))) (CC:[1025..1028] and) (NP (NN:[1029..1034] mouse) (NML-3 (NNS:[1035..1042] tissues))))))))) (.:[1042..1043] .))) ;sentence 7 Span:1044..1296 ;Our results from transfection assays of murine neuroblastoma cells with ;constructs expressing different regions of ovine PrP messenger RNA revealed ;the presence of sequences in the 3' untranslated region of the gene that ;modulate protein synthesis. ;[1092..1105]:malignancy-type:"neuroblastoma" ;[1167..1170]:gene-rna:"PrP" (SENT (S (NP-SBJ (NP (PRP$:[1044..1047] Our) (NNS:[1048..1055] results)) (PP (IN:[1057..1061] from) (NP (NP (NN:[1062..1074] transfection) (NNS:[1075..1081] assays)) (PP (IN:[1082..1084] of) (NP (NP (JJ:[1085..1091] murine) (NN:[1092..1105] neuroblastoma) (NNS:[1106..1111] cells)) (PP (IN:[1112..1116] with) (NP (NP (NNS:[1117..1127] constructs)) (VP (VBG:[1129..1139] expressing) (NP (NP (JJ:[1140..1149] different) (NNS:[1150..1157] regions)) (PP (IN:[1158..1160] of) (NP (JJ:[1161..1166] ovine) (NN:[1167..1170] PrP) (NN:[1171..1180] messenger) (NN:[1181..1184] RNA)))))))))))) (VP (VBD:[1185..1193] revealed) (NP (NP (DT:[1194..1197] the) (NN:[1198..1206] presence)) (PP (IN:[1207..1209] of) (NP (NNS:[1211..1220] sequences)))) (PP-LOC (IN:[1221..1223] in) (NP (NP (DT:[1224..1227] the) (NML (CD:[1228..1229] 3) (SYM:[1229..1230] ')) (JJ:[1231..1243] untranslated) (NN:[1244..1250] region)) (PP (IN:[1251..1253] of) (NP (NP (DT:[1254..1257] the) (NN:[1258..1262] gene)) (SBAR (WHNP-1 (WDT:[1263..1267] that)) (S (NP-SBJ-1 (-NONE-:[1267..1267] *T*)) (VP (VBP:[1268..1276] modulate) (NP (NN:[1277..1284] protein) (NN:[1286..1295] synthesis)))))))))) (.:[1295..1296] .))) ;section 8 Span:1300..1345 ;PMID: 10466827 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1300..1304] PMID) (::[1304..1305] :) (CD:[1306..1314] 10466827) (NN:[1315..1316] -LSB-) (NNP:[1316..1322] PubMed) (::[1323..1324] -) (NN:[1325..1332] indexed) (IN:[1333..1336] for) (NNP:[1337..1345] MEDLINE-RSB-)))