;PMID: 11901170 ;source_file_2849.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..41] = [t:0..41] ;1)sentence:[e:47..156] = [t:47..156] ;2)section:[e:160..214] = [t:160..214] ;3)section:[e:218..291] = [t:218..291] ;4)sentence:[e:295..476] = [t:295..476] ;5)sentence:[e:477..592] = [t:477..592] ;6)sentence:[e:593..683] = [t:593..683] ;7)sentence:[e:684..827] = [t:684..827] ;8)sentence:[e:828..1020] = [t:828..1020] ;9)sentence:[e:1021..1181] = [t:1021..1181] ;10)sentence:[e:1182..1292] = [t:1182..1292] ;11)section:[e:1296..1341] = [t:1296..1341] ;section 0 Span:0..41 ;J Cell Biol. 2002 Mar 18;156(6):1051-63. (SEC (FRAG (NNP:[0..1] J) (NNP:[2..6] Cell) (NNP:[7..11] Biol) (.:[11..12] .) ('':[14..18] 2002) (NNP:[19..22] Mar) (CD:[23..29] 18;156) (-LRB-:[29..30] -LRB-) (CD:[30..31] 6) (-RRB-:[31..32] -RRB-) (::[32..33] :) (CD:[33..37] 1051) (CD:[37..40] -63) (.:[40..41] .))) ;sentence 1 Span:47..156 ;Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons ; and enhances oxidative stress. ;[47..50]:gene-protein:"Tau" ;[101..104]:gene-protein:"APP" (SENT (S-HLN (NP-SBJ (SYM:[47..50] Tau)) (VP (VP (VBZ:[51..57] blocks) (NP (NP (NN:[58..65] traffic)) (PP (IN:[66..68] of) (NP (NP (NP (NNS:[69..79] organelles)) (,:[79..80] ,) (NP (NNS:[81..95] neurofilaments)) (,:[95..96] ,) (CC:[97..100] and) (NP (NN:[101..104] APP) (NNS:[105..113] vesicles))) (PP-LOC (IN:[114..116] in) (NP (NNS:[117..124] neurons))))))) (CC:[126..129] and) (VP (VBZ:[130..138] enhances) (NP (JJ:[139..148] oxidative) (NN:[149..155] stress)))) (.:[155..156] .))) ;section 2 Span:160..214 ;Stamer K, Vogel R, Thies E, Mandelkow E, Mandelkow EM. (SEC (FRAG (NNP:[160..166] Stamer) (NNP:[167..169] K,) (NNP:[170..175] Vogel) (NNP:[176..177] R) (,:[177..178] ,) (NNP:[179..184] Thies) (NNP:[185..186] E) (,:[186..187] ,) (NNP:[188..197] Mandelkow) (NNP:[198..199] E) (,:[199..200] ,) (NNP:[201..210] Mandelkow) (NN:[211..213] EM) (.:[213..214] .))) ;section 3 Span:218..291 ;Max-Planck-Unit for Structural Molecular Biology, 22607 Hamburg, Germany. (SEC (FRAG (JJ:[218..228] Max-Planck) (NN:[228..233] -Unit) (IN:[234..237] for) (NNP:[238..248] Structural) (NNP:[249..258] Molecular) (NNP:[259..266] Biology) (,:[266..267] ,) (CD:[268..273] 22607) (NNP:[274..281] Hamburg) (,:[281..282] ,) (NNP:[283..290] Germany) (.:[290..291] .))) ;sentence 4 Span:295..476 ;We studied the effect of microtubule-associated tau protein on trafficking of ; vesicles and organelles in primary cortical neurons, retinal ganglion cells, ;and neuroblastoma cells. ;[343..346]:gene-protein:"tau" ;[456..469]:malignancy-type:"neuroblastoma" (SENT (S (NP-SBJ (PRP:[295..297] We)) (VP (VBD:[298..305] studied) (NP (NP (DT:[306..309] the) (NN:[310..316] effect)) (PP (IN:[317..319] of) (NP (ADJP (NN:[320..331] microtubule) (HYPH:[331..332] -) (VBN:[332..342] associated)) (SYM:[343..346] tau) (NN:[347..354] protein))) (PP (IN:[355..357] on) (NP (NP (NN:[358..369] trafficking)) (PP (IN:[370..372] of) (NP (NP (NP (NNS:[374..382] vesicles)) (CC:[383..386] and) (NP (NNS:[387..397] organelles))) (PP-LOC (IN:[398..400] in) (NP (NP (JJ:[401..408] primary) (JJ:[409..417] cortical) (NNS:[418..425] neurons)) (,:[425..426] ,) (NP (JJ:[427..434] retinal) (NN:[435..443] ganglion) (NNS:[444..449] cells)) (,:[449..450] ,) (CC:[451..454] and) (NP (NN:[456..469] neuroblastoma) (NNS:[470..475] cells)))))))))) (.:[475..476] .))) ;sentence 5 Span:477..592 ;Tau inhibits kinesin-dependent transport of peroxisomes, neurofilaments, and ;Golgi-derived vesicles into neurites. ;[477..480]:gene-protein:"Tau" ;[490..497]:gene-protein:"kinesin" (SENT (S (NP-SBJ (SYM:[477..480] Tau)) (VP (VBZ:[481..489] inhibits) (NP (NP (ADJP (NN:[490..497] kinesin) (HYPH:[497..498] -) (JJ:[498..507] dependent)) (NN:[508..517] transport)) (PP (IN:[518..520] of) (NP (NP (NNS:[521..532] peroxisomes)) (,:[532..533] ,) (NP (NNS:[535..549] neurofilaments)) (,:[549..550] ,) (CC:[551..554] and) (NP (ADJP (NNP:[555..560] Golgi) (HYPH:[560..561] -) (VBN:[561..568] derived)) (NNS:[569..577] vesicles)))) (PP (IN:[578..582] into) (NP (NNS:[583..591] neurites))))) (.:[591..592] .))) ;sentence 6 Span:593..683 ;Loss of peroxisomes makes cells vulnerable to oxidative stress and leads to ;degeneration. (SENT (S (NP-SBJ (NP (NN:[593..597] Loss)) (PP (IN:[598..600] of) (NP (NNS:[601..612] peroxisomes)))) (VP (VP (VBZ:[614..619] makes) (S (NP-SBJ (NNS:[620..625] cells)) (ADJP-PRD (JJ:[626..636] vulnerable) (PP (TO:[637..639] to) (NP (JJ:[640..649] oxidative) (NN:[650..656] stress)))))) (CC:[657..660] and) (VP (VBZ:[661..666] leads) (PP (TO:[667..669] to) (NP (NN:[670..682] degeneration))))) (.:[682..683] .))) ;sentence 7 Span:684..827 ;In particular, tau inhibits transport of amyloid precursor protein (APP) ;into axons and dendrites, causing its accumulation in the cell body. ;[700..703]:gene-protein:"tau" ;[726..751]:gene-protein:"amyloid precursor protein" ;[753..756]:gene-protein:"APP" (SENT (S (PP (IN:[684..686] In) (NP (JJ:[688..698] particular))) (,:[698..699] ,) (NP-SBJ (SYM:[700..703] tau)) (VP (VBZ:[704..712] inhibits) (NP (NP (NN:[713..722] transport)) (PP (IN:[723..725] of) (NP (NP (JJ:[726..733] amyloid) (NN:[734..743] precursor) (NN:[744..751] protein)) (NP (-LRB-:[752..753] -LRB-) (NN:[753..756] APP) (-RRB-:[756..757] -RRB-)))) (PP (IN:[758..762] into) (NP (NP (NNS:[763..768] axons)) (CC:[770..773] and) (NP (NNS:[774..783] dendrites))))) (,:[783..784] ,) (S-ADV (NP-SBJ (-NONE-:[784..784] *)) (VP (VBG:[785..792] causing) (NP (NP (PRP$:[793..796] its) (NN:[797..809] accumulation)) (PP (IN:[810..812] in) (NP (DT:[813..816] the) (NN:[817..821] cell) (NN:[822..826] body))))))) (.:[826..827] .))) ;sentence 8 Span:828..1020 ;APP tagged with yellow fluorescent protein and transfected by adenovirus ;associates with vesicles moving rapidly forward in the axon (approximately ;80%) and slowly back (approximately 20%). ;[828..831]:gene-protein:"APP" ;[844..871]:gene-protein:"yellow fluorescent protein" (SENT (S (NP-SBJ (NP (NN:[828..831] APP)) (VP (VP (VBN:[832..838] tagged) (NP (-NONE-:[838..838] *)) (PP (IN:[839..843] with) (NP (JJ:[844..850] yellow) (JJ:[852..863] fluorescent) (NN:[864..871] protein)))) (CC:[872..875] and) (VP (VBN:[876..887] transfected) (NP (-NONE-:[887..887] *)) (PP-MNR (IN:[888..890] by) (NP (NN:[891..901] adenovirus)))))) (VP (VBZ:[902..912] associates) (PP-CLR (IN:[913..917] with) (NP (NP (NNS:[918..926] vesicles)) (VP (VP (VBG:[928..934] moving) (ADVP=1 (RB:[935..942] rapidly)) (ADVP-DIR=2 (RB:[943..950] forward)) (PP-LOC (IN:[951..953] in) (NP (DT:[954..957] the) (NN:[958..962] axon) (PRN (-LRB-:[963..964] -LRB-) (NP (QP (RB:[964..977] approximately) (CD:[978..980] 80)) (NN:[980..981] %)) (-RRB-:[981..982] -RRB-))))) (CC:[983..986] and) (VP (ADVP=1 (RB:[987..993] slowly)) (ADVP-DIR=2 (RB:[994..998] back)) (PRN (-LRB-:[1000..1001] -LRB-) (NP (QP (RB:[1001..1014] approximately) (CD:[1015..1017] 20)) (NN:[1017..1018] %)) (-RRB-:[1018..1019] -RRB-))))))) (.:[1019..1020] .))) ;sentence 9 Span:1021..1181 ;Both movements are strongly inhibited by cotransfection with fluorescently ;tagged tau (cyan fluorescent protein-tau) as seen by two-color confocal ;microscopy. ;[1104..1107]:gene-protein:"tau" ;[1109..1133]:gene-protein:"cyan fluorescent protein" ;[1134..1137]:gene-protein:"tau" (SENT (S (NP-SBJ-1 (DT:[1021..1025] Both) (NNS:[1026..1035] movements)) (VP (VBP:[1036..1039] are) (ADVP (RB:[1040..1048] strongly)) (VP (VBN:[1049..1058] inhibited) (NP-1 (-NONE-:[1058..1058] *)) (PP (IN:[1059..1061] by) (NP-LGS (NP (NN:[1062..1076] cotransfection)) (PP (IN:[1078..1082] with) (NP (ADJP (RB:[1083..1096] fluorescently) (VBN:[1097..1103] tagged)) (SYM:[1104..1107] tau) (PRN (-LRB-:[1108..1109] -LRB-) (NP (NN:[1109..1113] cyan) (NML (JJ:[1114..1125] fluorescent) (NN:[1126..1133] protein)) (HYPH:[1133..1134] -) (SYM:[1134..1137] tau)) (-RRB-:[1137..1138] -RRB-)))))) (SBAR (IN:[1139..1141] as) (S (NP-SBJ-2 (-NONE-:[1141..1141] *)) (VP (VBN:[1142..1146] seen) (NP-2 (-NONE-:[1146..1146] *)) (PP (IN:[1147..1149] by) (NP (NML (CD:[1151..1154] two) (HYPH:[1154..1155] -) (NN:[1155..1160] color)) (JJ:[1161..1169] confocal) (NN:[1170..1180] microscopy)))))))) (.:[1180..1181] .))) ;sentence 10 Span:1182..1292 ;The data suggests a linkage between tau and APP trafficking, which may be ;significant in Alzheimer's disease. ;[1218..1221]:gene-protein:"tau" ;[1226..1229]:gene-protein:"APP" (SENT (S (NP-SBJ (DT:[1182..1185] The) (NN:[1186..1190] data)) (VP (VBZ:[1191..1199] suggests) (NP (NP (NP (DT:[1200..1201] a) (NN:[1202..1209] linkage)) (PP (IN:[1210..1217] between) (NP (NP (SYM:[1218..1221] tau)) (CC:[1222..1225] and) (NP (NN:[1226..1229] APP) (NN:[1231..1242] trafficking))))) (,:[1242..1243] ,) (SBAR (WHNP-1 (WDT:[1244..1249] which)) (S (NP-SBJ-1 (-NONE-:[1249..1249] *T*)) (VP (MD:[1250..1253] may) (VP (VB:[1254..1256] be) (ADJP-PRD (JJ:[1257..1268] significant)) (PP (IN:[1269..1271] in) (NP (NP (NNP:[1272..1281] Alzheimer) (POS:[1281..1283] 's)) (NN:[1284..1291] disease))))))))) (.:[1291..1292] .))) ;section 11 Span:1296..1341 ;PMID: 11901170 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1296..1300] PMID) (::[1300..1301] :) (CD:[1302..1310] 11901170) (NN:[1311..1312] -LSB-) (NNP:[1312..1318] PubMed) (::[1319..1320] -) (NN:[1321..1328] indexed) (IN:[1329..1332] for) (NNP:[1333..1341] MEDLINE-RSB-)))