;PMID: 1314950 ;source_file_2848.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..40] = [t:0..40] ;1)sentence:[e:46..179] = [t:46..179] ;2)section:[e:183..224] = [t:183..224] ;3)section:[e:228..330] = [t:228..330] ;4)sentence:[e:334..571] = [t:334..571] ;5)sentence:[e:572..716] = [t:572..716] ;6)sentence:[e:717..880] = [t:717..880] ;7)sentence:[e:881..1038] = [t:881..1038] ;8)sentence:[e:1039..1179] = [t:1039..1179] ;9)sentence:[e:1180..1288] = [t:1180..1288] ;10)section:[e:1292..1336] = [t:1292..1336] ;section 0 Span:0..40 ;Mol Cell Biol. 1992 May;12(5):2193-202. (SEC (FRAG (NNP:[0..3] Mol) (NNP:[4..8] Cell) (NNP:[9..13] Biol) (.:[13..14] .) (CD:[16..20] 1992) (NNP:[21..24] May) (CD:[24..28] ;12-LRB-) (CD:[28..29] 5) (-RRB-:[29..30] -RRB-) (::[30..31] :) (CD:[31..35] 2193) (CD:[35..39] -202) (.:[39..40] .))) ;sentence 1 Span:46..179 ;Basic fibroblast growth factor enhances nerve growth factor receptor gene ;promoter activity in human neuroblastoma cell line CHP100. ;[46..76]:gene-protein:"Basic fibroblast growth factor" ;[86..114]:gene-rna:"nerve growth factor receptor" ;[148..161]:malignancy-type:"neuroblastoma" (SENT (S (NP-SBJ (JJ:[46..51] Basic) (NN:[52..62] fibroblast) (NN:[63..69] growth) (NN:[70..76] factor)) (VP (VBZ:[77..85] enhances) (NP (NML (NN:[86..91] nerve) (NN:[92..98] growth) (NN:[99..105] factor) (NN:[106..114] receptor)) (NML (NN:[115..119] gene) (NN:[121..129] promoter)) (NN:[130..138] activity)) (PP (IN:[139..141] in) (NP (JJ:[142..147] human) (NN:[148..161] neuroblastoma) (NML (NN:[162..166] cell) (NN:[167..171] line)) (NN:[172..178] CHP100)))) (.:[178..179] .))) ;section 2 Span:183..224 ;Taiji M, Taiji K, Deyerle KL, Bothwell M. (SEC (FRAG (NNP:[183..188] Taiji) (NNP:[189..191] M,) (NNP:[192..197] Taiji) (NNP:[198..200] K,) (NNP:[201..208] Deyerle) (NNP:[209..211] KL) (,:[211..212] ,) (NNP:[213..221] Bothwell) (NNP:[222..224] M.))) ;section 3 Span:228..330 ;Department of Physiology and Biophysics, School of Medicine, University of ;Washington, Seattle 98195. (SEC (FRAG (NNP:[228..238] Department) (IN:[239..241] of) (NNP:[242..252] Physiology) (CC:[253..256] and) (NNP:[257..267] Biophysics) (,:[267..268] ,) (NNP:[269..275] School) (IN:[276..278] of) (NNP:[279..287] Medicine) (,:[287..288] ,) (NNP:[289..299] University) (IN:[300..302] of) (NNP:[304..314] Washington) (,:[314..315] ,) (NNP:[316..323] Seattle) (CD:[324..329] 98195) (.:[329..330] .))) ;sentence 4 Span:334..571 ;The human neuroblastoma cell line CHP100 provides a useful model system in ;which to study the molecular mechanisms of transcriptional regulation of the ; low-affinity nerve growth factor receptor (NGFR) gene during neuronal ;development. ;[344..357]:malignancy-type:"neuroblastoma" ;[501..529]:gene-rna:"nerve growth factor receptor" ;[531..535]:gene-rna:"NGFR" (SENT (S (NP-SBJ (DT:[334..337] The) (JJ:[338..343] human) (NN:[344..357] neuroblastoma) (NML (NN:[358..362] cell) (NN:[363..367] line)) (NN:[368..374] CHP100)) (VP (VBZ:[375..383] provides) (NP (NP (DT:[384..385] a) (JJ:[386..392] useful) (NN:[393..398] model) (NN:[399..405] system)) (SBAR (WHPP-1 (IN:[406..408] in) (WHNP (WDT:[409..414] which))) (S (NP-SBJ (-NONE-:[414..414] *)) (VP (TO:[416..418] to) (VP (VB:[419..424] study) (NP (NP (DT:[425..428] the) (JJ:[429..438] molecular) (NNS:[439..449] mechanisms)) (PP (IN:[450..452] of) (NP (NP (JJ:[453..468] transcriptional) (NN:[469..479] regulation)) (PP (IN:[480..482] of) (NP (DT:[483..486] the) (NML (JJ:[488..491] low) (HYPH:[491..492] -) (NN:[492..500] affinity)) (NML (NML (NN:[501..506] nerve) (NN:[507..513] growth) (NN:[514..520] factor) (NN:[521..529] receptor)) (NML (-LRB-:[530..531] -LRB-) (NN:[531..535] NGFR) (-RRB-:[535..536] -RRB-))) (NN:[537..541] gene))) (PP-TMP (IN:[542..548] during) (NP (JJ:[549..557] neuronal) (NN:[559..570] development)))))) (PP-1 (-NONE-:[570..570] *T*)))))))) (.:[570..571] .))) ;sentence 5 Span:572..716 ;Basic fibroblast growth factor (bFGF) induced morphological changes in ;CHP100 cells, including flattening of cell bodies and neurite outgrowth. ;[572..602]:gene-protein:"Basic fibroblast growth factor" ;[604..608]:gene-protein:"bFGF" (SENT (S (NP-SBJ (NP (JJ:[572..577] Basic) (NN:[578..588] fibroblast) (NN:[589..595] growth) (NN:[596..602] factor)) (NP (-LRB-:[603..604] -LRB-) (NN:[604..608] bFGF) (-RRB-:[608..609] -RRB-))) (VP (VBD:[610..617] induced) (NP (JJ:[618..631] morphological) (NNS:[632..639] changes)) (PP-LOC (IN:[641..643] in) (NP (NN:[644..650] CHP100) (NNS:[651..656] cells))) (,:[656..657] ,) (PP (VBG:[658..667] including) (NP (NP (NP (NN:[668..678] flattening)) (PP (IN:[679..681] of) (NP (NN:[682..686] cell) (NNS:[687..693] bodies)))) (CC:[694..697] and) (NP (NN:[698..705] neurite) (NN:[706..715] outgrowth))))) (.:[715..716] .))) ;sentence 6 Span:717..880 ;bFGF also increased p75NGFR immunoreactivity, as assessed by ;immunocytochemistry, and increased p75NGFR mRNA levels, as assessed by ;Northern (RNA) blot analysis. ;[717..721]:gene-protein:"bFGF" ;[738..745]:gene-protein:"p75NGFR" ;[815..822]:gene-rna:"p75NGFR" (SENT (S (NP-SBJ (NN:[717..721] bFGF)) (VP (VP (ADVP (RB:[723..727] also)) (VBD:[728..737] increased) (NP (NN:[738..745] p75NGFR) (NN:[746..762] immunoreactivity)) (,:[762..763] ,) (SBAR-ADV (IN:[764..766] as) (S (NP-SBJ (-NONE-:[766..766] *)) (VP (VBN:[767..775] assessed) (NP (-NONE-:[775..775] *)) (PP-MNR (IN:[776..778] by) (NP (NN:[779..798] immunocytochemistry))))))) (,:[798..799] ,) (CC:[800..803] and) (VP (VBD:[805..814] increased) (NP (NN:[815..822] p75NGFR) (NN:[823..827] mRNA) (NNS:[828..834] levels)) (,:[834..835] ,) (SBAR-ADV (IN:[836..838] as) (S (NP-SBJ-1 (-NONE-:[838..838] *)) (VP (VBN:[839..847] assessed) (NP-1 (-NONE-:[847..847] *)) (PP (IN:[848..850] by) (NP-LGS (JJ:[851..859] Northern) (NML (-LRB-:[860..861] -LRB-) (NN:[861..864] RNA) (-RRB-:[864..865] -RRB-)) (NN:[866..870] blot) (NN:[871..879] analysis)))))))) (.:[879..880] .))) ;sentence 7 Span:881..1038 ;A chimeric gene consisting of 6.7 kb of the 5'-flanking region of the human ;NGFR gene linked to the chloramphenicol acetyltransferase gene was ;constructed. ;[958..962]:gene-rna:"NGFR" ;[983..1016]:gene-rna:"chloramphenicol acetyltransferase" (SENT (S (NP-SBJ-1 (NP (DT:[881..882] A) (JJ:[884..892] chimeric) (NN:[893..897] gene)) (VP (VBG:[898..908] consisting) (PP-CLR (IN:[909..911] of) (NP (NP (CD:[912..915] 6.7) (NN:[916..918] kb)) (PP (IN:[919..921] of) (NP (NP (NP (DT:[922..925] the) (ADJP (NML (CD:[926..927] 5) (SYM:[927..928] ')) (HYPH:[928..929] -) (NN:[929..937] flanking)) (NN:[938..944] region)) (PP (IN:[945..947] of) (NP (DT:[948..951] the) (JJ:[952..957] human) (NN:[958..962] NGFR) (NN:[964..968] gene)))) (VP (VBN:[969..975] linked) (PP (TO:[976..978] to) (NP (DT:[979..982] the) (NN:[983..998] chloramphenicol) (NN:[999..1016] acetyltransferase) (NN:[1017..1021] gene)))))))))) (VP (VBD:[1022..1025] was) (VP (VBN:[1026..1037] constructed) (NP-1 (-NONE-:[1037..1037] *)))) (.:[1037..1038] .))) ;sentence 8 Span:1039..1179 ;In stable transformants of CHP100 cells, 10 ng of bFGF per ml induced an ;eightfold increase in chloramphenicol acetyltransferase activity. ;[1090..1094]:gene-protein:"bFGF" ;[1136..1169]:gene-protein:"chloramphenicol acetyltransferase" (SENT (S (PP-LOC (IN:[1039..1041] In) (NP (NP (JJ:[1043..1049] stable) (NNS:[1050..1063] transformants)) (PP (IN:[1064..1066] of) (NP (NN:[1067..1073] CHP100) (NNS:[1074..1079] cells))))) (,:[1079..1080] ,) (NP-SBJ (NP (CD:[1081..1083] 10) (NN:[1084..1086] ng)) (PP (IN:[1087..1089] of) (NP (NN:[1090..1094] bFGF))) (PP (IN:[1095..1098] per) (NP (NN:[1099..1101] ml)))) (VP (VBD:[1102..1109] induced) (NP (NP (DT:[1110..1112] an) (JJ:[1113..1122] eightfold) (NN:[1124..1132] increase)) (PP (IN:[1133..1135] in) (NP (NN:[1136..1151] chloramphenicol) (NN:[1152..1169] acetyltransferase) (NN:[1170..1178] activity))))) (.:[1178..1179] .))) ;sentence 9 Span:1180..1288 ;These results indicate that upstream elements of the NGFR gene mediate ;transcriptional regulation by bFGF. ;[1234..1238]:gene-rna:"NGFR" ;[1283..1287]:gene-protein:"bFGF" (SENT (S (NP-SBJ (DT:[1180..1185] These) (NNS:[1186..1193] results)) (VP (VBP:[1194..1202] indicate) (SBAR (IN:[1204..1208] that) (S (NP-SBJ (NP (JJ:[1209..1217] upstream) (NNS:[1218..1226] elements)) (PP (IN:[1227..1229] of) (NP (DT:[1230..1233] the) (NN:[1234..1238] NGFR) (NN:[1239..1243] gene)))) (VP (VBP:[1244..1251] mediate) (NP (NP (JJ:[1252..1267] transcriptional) (NN:[1268..1278] regulation)) (PP (IN:[1279..1281] by) (NP (NN:[1283..1287] bFGF)))))))) (.:[1287..1288] .))) ;section 10 Span:1292..1336 ;PMID: 1314950 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1292..1296] PMID) (::[1296..1297] :) (CD:[1298..1305] 1314950) (NN:[1306..1307] -LSB-) (NNP:[1307..1313] PubMed) (::[1314..1315] -) (NN:[1316..1323] indexed) (IN:[1324..1327] for) (NNP:[1328..1336] MEDLINE-RSB-)))