;PMID: 11410369 ;source_file_2840.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..32] = [t:0..32] ;1)sentence:[e:38..123] = [t:38..123] ;2)section:[e:127..155] = [t:127..155] ;3)section:[e:159..305] = [t:159..305] ;4)sentence:[e:309..428] = [t:309..428] ;5)sentence:[e:429..581] = [t:429..581] ;6)sentence:[e:582..666] = [t:582..666] ;7)sentence:[e:667..793] = [t:667..793] ;8)sentence:[e:794..911] = [t:794..911] ;9)sentence:[e:912..979] = [t:912..979] ;10)sentence:[e:980..1075] = [t:980..1075] ;11)sentence:[e:1076..1129] = [t:1076..1129] ;12)sentence:[e:1130..1268] = [t:1130..1268] ;13)section:[e:1272..1317] = [t:1272..1317] ;section 0 Span:0..32 ;Gene. 2001 Jun 13;271(1):87-92. (SEC (FRAG (NNP:[0..4] Gene) (.:[4..5] .) (CD:[7..11] 2001) (NNP:[12..15] Jun) (CD:[16..22] 13;271) (-LRB-:[22..23] -LRB-) (CD:[23..24] 1) (-RRB-:[24..25] -RRB-) (::[25..26] :) (CD:[26..28] 87) (::[28..29] -) (CD:[29..31] 92) (.:[31..32] .))) ;sentence 1 Span:38..123 ;Structure of the human M(2) muscarinic acetylcholine receptor gene and its ;promoter. ;[61..99]:gene-rna:"M(2) muscarinic acetylcholine receptor" (SENT (NP-HLN (NP (NN:[38..47] Structure)) (PP (IN:[48..50] of) (NP (NP (DT:[51..54] the) (JJ:[55..60] human) (NML (NN:[61..65] M-LRB-2-RRB-) (JJ:[66..76] muscarinic) (NN:[77..90] acetylcholine) (NN:[91..99] receptor)) (NN:[100..104] gene)) (CC:[105..108] and) (NP (PRP$:[109..112] its) (NN:[114..122] promoter)))) (.:[122..123] .))) ;section 2 Span:127..155 ;Zhou C, Fryer AD, Jacoby DB. (SEC (FRAG (NNP:[127..131] Zhou) (NNP:[132..133] C) (,:[133..134] ,) (NNP:[135..140] Fryer) (NNP:[141..143] AD) (,:[143..144] ,) (NNP:[145..151] Jacoby) (NNP:[152..155] DB.))) ;section 3 Span:159..305 ;Division of Pulmonary and Critical Care Medicine, School of Medicine, Johns ;Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205, USA. (SEC (FRAG (NNP:[159..167] Division) (IN:[168..170] of) (NNP:[171..180] Pulmonary) (CC:[181..184] and) (NNP:[185..193] Critical) (NNP:[194..198] Care) (NNP:[199..207] Medicine) (,:[207..208] ,) (NNP:[209..215] School) (IN:[216..218] of) (NNP:[219..227] Medicine) (,:[227..228] ,) (NNP:[229..234] Johns) (NNP:[236..243] Hopkins) (NNP:[244..254] University) (,:[254..255] ,) (CD:[256..259] 615) (NNP:[260..265] North) (NNP:[266..271] Wolfe) (NNP:[272..278] Street) (,:[278..279] ,) (NNP:[280..289] Baltimore) (,:[289..290] ,) (NNP:[291..293] MD) (CD:[294..299] 21205) (,:[299..300] ,) (NNP:[301..304] USA) (.:[304..305] .))) ;sentence 4 Span:309..428 ;The M(2) muscarinic receptor inhibits the release of acetylcholine from ;cholinergic fibers in the lungs and elsewhere. ;[313..337]:gene-protein:"M(2) muscarinic receptor" (SENT (S (NP-SBJ (DT:[309..312] The) (NN:[313..317] M-LRB-2-RRB-) (JJ:[318..328] muscarinic) (NN:[329..337] receptor)) (VP (VBZ:[338..346] inhibits) (NP (NP (DT:[347..350] the) (NN:[351..358] release)) (PP (IN:[359..361] of) (NP (NN:[362..375] acetylcholine))) (PP (IN:[376..380] from) (NP (JJ:[382..393] cholinergic) (NNS:[394..400] fibers)))) (UCP-LOC (PP (IN:[401..403] in) (NP (DT:[404..407] the) (NNS:[408..413] lungs))) (CC:[414..417] and) (ADVP (RB:[418..427] elsewhere)))) (.:[427..428] .))) ;sentence 5 Span:429..581 ;In airway parasympathetic neurons, M(2) receptor expression is decreased by ;viral infections and by interferon-gamma, increasing actylcholine release. ;[465..478]:gene-generic:"M(2) receptor" ;[531..547]:gene-protein:"interferon-gamma" (SENT (S (PP-LOC (IN:[429..431] In) (NP (NN:[432..438] airway) (JJ:[439..454] parasympathetic) (NNS:[456..463] neurons))) (,:[463..464] ,) (NP-SBJ-1 (NML (NN:[465..469] M-LRB-2-RRB-) (NN:[470..478] receptor)) (NN:[479..489] expression)) (VP (VBZ:[490..492] is) (VP (VBN:[493..502] decreased) (NP-1 (-NONE-:[502..502] *)) (PP (PP (IN:[503..505] by) (NP-LGS (JJ:[506..511] viral) (NNS:[512..522] infections))) (CC:[523..526] and) (PP (IN:[527..529] by) (NP-LGS (NN:[531..541] interferon) (HYPH:[541..542] -) (SYM:[542..547] gamma)))) (,:[547..548] ,) (S-ADV (NP-SBJ (-NONE-:[548..548] *)) (VP (VBG:[549..559] increasing) (NP (NN:[560..572] actylcholine) (NN:[573..580] release)))))) (.:[580..581] .))) ;sentence 6 Span:582..666 ;Dexamethasone increases M(2) receptor expression, decreasing acetylcholine ;release. ;[606..620]:gene-generic:"M(2) receptor" (SENT (S (NP-SBJ (NN:[582..595] Dexamethasone)) (VP (VBZ:[596..605] increases) (NP (NML (NN:[606..610] M-LRB-2-RRB-) (NN:[612..620] receptor)) (NN:[621..631] expression)) (,:[631..632] ,) (S-ADV (NP-SBJ (-NONE-:[632..632] *)) (VP (VBG:[633..643] decreasing) (NP (NN:[644..657] acetylcholine) (NN:[658..665] release))))) (.:[665..666] .))) ;sentence 7 Span:667..793 ;We carried out 5' rapid amplification of cDNA ends beginning with mRNA from ;human heart and IMR32 human neuroblastoma cells. ;[773..786]:malignancy-type:"neuroblastoma" (SENT (S (NP-SBJ (PRP:[667..669] We)) (VP (VBD:[670..677] carried) (PRT (RP:[678..681] out)) (NP (NP (NML (CD:[682..683] 5) (SYM:[683..684] ')) (JJ:[685..690] rapid) (NN:[692..705] amplification)) (PP (IN:[706..708] of) (NP (NN:[709..713] cDNA) (NNS:[714..718] ends)))) (S-ADV (NP-SBJ (-NONE-:[718..718] *)) (VP (VBG:[719..728] beginning) (PP (IN:[729..733] with) (NP (NP (NN:[734..738] mRNA)) (PP (IN:[739..743] from) (NP (NP (JJ:[744..749] human) (NN:[750..755] heart) (NML-1 (-NONE-:[755..755] *P*))) (CC:[756..759] and) (NP (NN:[760..765] IMR32) (JJ:[766..771] human) (NN:[773..786] neuroblastoma) (NML-1 (NNS:[787..792] cells)))))))))) (.:[792..793] .))) ;sentence 8 Span:794..911 ;This demonstrated a 5' UTR of 100 BP, corresponding to two sequences on ;chromosome 7, separated by a 22.6 kB intron. (SENT (S (NP-SBJ (DT:[794..798] This)) (VP (VBD:[799..811] demonstrated) (NP (NP (NP (DT:[812..813] a) (NML (CD:[814..815] 5) (SYM:[815..816] ')) (NN:[817..820] UTR)) (PP (IN:[821..823] of) (NP (CD:[824..827] 100) (NN:[828..830] BP)))) (,:[830..831] ,) (VP (VBG:[832..845] corresponding) (PP-CLR (TO:[846..848] to) (NP (NP (NP (CD:[849..852] two) (NNS:[854..863] sequences)) (PP-LOC (IN:[864..866] on) (NP (NN:[867..877] chromosome) (CD:[878..879] 7)))) (,:[879..880] ,) (VP (VBN:[881..890] separated) (NP (-NONE-:[890..890] *)) (PP (IN:[891..893] by) (NP-LGS (DT:[894..895] a) (NML (CD:[896..900] 22.6) (NN:[901..903] kB)) (NN:[904..910] intron))))))))) (.:[910..911] .))) ;sentence 9 Span:912..979 ;The splice acceptor site is at -45 relative to the initiating atg. (SENT (S (NP-SBJ (DT:[912..915] The) (NML (NN:[916..922] splice) (NN:[923..931] acceptor)) (NN:[933..937] site)) (VP (VBZ:[938..940] is) (PP-PRD (IN:[941..943] at) (NP (SYM:[944..945] -) (CD:[945..947] 45))) (S-ADV (NP-SBJ (-NONE-:[947..947] *)) (ADJP-PRD (JJ:[948..956] relative) (PP (TO:[957..959] to) (NP (DT:[960..963] the) (VBG:[964..974] initiating) (NN:[975..978] atg)))))) (.:[978..979] .))) ;sentence 10 Span:980..1075 ;The 3000 BP upstream of 5' RACE product were subcloned into a pGL3 ;luciferase reporter vector. (SENT (S (NP-SBJ-1 (NP (DT:[980..983] The) (CD:[984..988] 3000) (NN:[989..991] BP)) (ADJP (JJ:[992..1000] upstream) (PP (IN:[1001..1003] of) (NP (NML (CD:[1004..1005] 5) (SYM:[1005..1006] ')) (NN:[1007..1011] RACE) (NN:[1013..1020] product))))) (VP (VBD:[1021..1025] were) (VP (VBN:[1026..1035] subcloned) (NP-1 (-NONE-:[1035..1035] *)) (PP (IN:[1036..1040] into) (NP (DT:[1041..1042] a) (NML (NN:[1043..1047] pGL3) (NN:[1048..1058] luciferase)) (NN:[1059..1067] reporter) (NN:[1068..1074] vector))))) (.:[1074..1075] .))) ;sentence 11 Span:1076..1129 ;Deletional constructs were expressed in IMR32 cells. (SENT (S (NP-SBJ-1 (JJ:[1076..1086] Deletional) (NNS:[1088..1098] constructs)) (VP (VBD:[1099..1103] were) (VP (VBN:[1104..1113] expressed) (NP-1 (-NONE-:[1113..1113] *)) (PP-LOC (IN:[1114..1116] in) (NP (NN:[1117..1122] IMR32) (NNS:[1123..1128] cells))))) (.:[1128..1129] .))) ;sentence 12 Span:1130..1268 ;These demonstrated that 412 BP provided full expression of the reporter ;gene, and suggested a repressor element between -1848 and -1510. (SENT (S (NP-SBJ (DT:[1130..1135] These)) (VP (VP (VBD:[1136..1148] demonstrated) (SBAR (IN:[1149..1153] that) (S (NP-SBJ (CD:[1154..1157] 412) (NN:[1158..1160] BP)) (VP (VBD:[1162..1170] provided) (NP (NP (JJ:[1171..1175] full) (NN:[1176..1186] expression)) (PP (IN:[1187..1189] of) (NP (DT:[1190..1193] the) (NN:[1194..1202] reporter) (NN:[1203..1207] gene)))))))) (,:[1207..1208] ,) (CC:[1209..1212] and) (VP (VBD:[1213..1222] suggested) (NP (NP (DT:[1223..1224] a) (NN:[1225..1234] repressor) (NN:[1235..1242] element)) (PP (IN:[1244..1251] between) (NP (NP (SYM:[1252..1253] -) (CD:[1253..1257] 1848)) (CC:[1258..1261] and) (NP (SYM:[1262..1263] -) (CD:[1263..1267] 1510))))))) (.:[1267..1268] .))) ;section 13 Span:1272..1317 ;PMID: 11410369 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1272..1276] PMID) (::[1276..1277] :) (CD:[1278..1286] 11410369) (NN:[1287..1288] -LSB-) (NNP:[1288..1294] PubMed) (::[1295..1296] -) (NN:[1297..1304] indexed) (IN:[1305..1308] for) (NNP:[1309..1317] MEDLINE-RSB-)))