;PMID: 9761241 ;source_file_1397.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..33] = [t:0..33] ;1)sentence:[e:38..150] = [t:38..150] ;2)section:[e:154..248] = [t:154..248] ;3)section:[e:252..399] = [t:252..399] ;4)sentence:[e:403..606] = [t:403..606] ;5)sentence:[e:607..807] = [t:607..807] ;6)sentence:[e:808..965] = [t:808..965] ;7)sentence:[e:966..1193] = [t:966..1193] ;8)section:[e:1197..1241] = [t:1197..1241] ;section 0 Span:0..33 ;Clin Chem 1998 Oct;44(10):2103-7 (SEC (FRAG (DT:[0..4] Clin) (JJ:[5..9] Chem) (CD:[11..15] 1998) (IN:[16..23] Oct;44-LRB-) (CD:[23..25] 10) (HYPH:[25..26] -RRB-) (CD:[26..31] :2103) (HYPH:[31..32] -) (CD:[32..33] 7))) ;sentence 1 Span:38..150 ;K-ras mutations in stools and tissue samples from patients with malignant and ; nonmalignant pancreatic diseases. ;[38..43]:gene-rna:"K-ras" ;[102..111]...[130..149]:malignancy:"malignant"..."pancreatic diseases" (SENT (NP-HLN (NP-SBJ (NN:[38..43] K-ras) (NNS:[44..53] mutations)) (PP-LOC (IN:[54..56] in) (NP (NP (NP (NNS:[57..63] stools)) (CC:[64..67] and) (NP (NN:[68..74] tissue) (NNS:[75..82] samples))) (PP (IN:[83..87] from) (NP (NP (NNS:[88..96] patients)) (PP (IN:[97..101] with) (NP (NP (JJ:[102..111] malignant) (NML-1 (-NONE-:[111..111] *P*))) (CC:[112..115] and) (NP (JJ:[117..129] nonmalignant) (NML-1 (JJ:[130..140] pancreatic) (NNS:[141..149] diseases))))))))) (.:[149..150] .))) ;section 2 Span:154..248 ;Berndt C, Haubold K, Wenger F, Brux B, Muller J, Bendzko P, Hillebrand T, ;Kottgen E, Zanow J. (SEC (FRAG (NNP:[154..160] Berndt) (NNP:[161..162] C) (,:[162..163] ,) (NNP:[164..171] Haubold) (NNP:[172..173] K) (NNP:[173..174] ,) (NNP:[175..181] Wenger) (NNP:[182..183] F) (,:[183..184] ,) (DT:[185..189] Brux) (NNP:[190..191] B) (,:[191..192] ,) (NNP:[193..199] Muller) (NNP:[200..202] J,) (NNP:[203..210] Bendzko) (NNP:[211..212] P) (VBD:[212..213] ,) (NNP:[214..224] Hillebrand) (NNP:[225..226] T) (,:[226..227] ,) (NNP:[229..236] Kottgen) (NNP:[237..238] E) (,:[238..239] ,) (NNP:[240..245] Zanow) (NNP:[246..248] J.))) ;section 3 Span:252..399 ;Institute of Laboratory Medicine and Pathobiochemistry, Medical Faculty ;Charite, Humboldt-University, Berlin, Germany. christoph.berndt@charite.de (SEC (FRAG (NNP:[252..261] Institute) (NNP:[262..264] of) (NNP:[265..275] Laboratory) (NNP:[276..284] Medicine) (VBD:[285..288] and) (NNP:[289..306] Pathobiochemistry) (,:[306..307] ,) (NNP:[308..315] Medical) (NNP:[316..323] Faculty) (NNP:[324..331] Charite) (,:[331..332] ,) (NNP:[334..342] Humboldt) (HYPH:[342..343] -) (NNP:[343..353] University) (,:[353..354] ,) (NNP:[355..361] Berlin) (,:[361..362] ,) (NNP:[363..370] Germany) (.:[370..371] .) ('':[372..381] christoph) (VBD:[381..396] .berndt@charite) (.:[396..399] .de))) ;sentence 4 Span:403..606 ;Mutant-enriched PCR and reverse dot blot hybridization in microplates were ;applied for examining K-ras status in stools and tissue samples from patients ; with pancreatic tumors and chronic pancreatitis. ;[501..506]:gene-rna:"K-ras" ;[563..580]:malignancy:"pancreatic tumors" (SENT (S (NP-SBJ-1 (NP (ADJP (NN:[403..409] Mutant) (HYPH:[409..410] -) (VBN:[410..418] enriched)) (NN:[419..422] PCR)) (CC:[423..426] and) (NP (NP (JJ:[427..434] reverse) (NML (NN:[435..438] dot) (NN:[439..443] blot)) (NN:[444..457] hybridization)) (PP (IN:[458..460] in) (NP (NNS:[461..472] microplates))))) (VP (VBD:[473..477] were) (VP (VBN:[479..486] applied) (NP-1 (-NONE-:[486..486] *)) (PP-PRP (IN:[487..490] for) (S-NOM (NP-SBJ (-NONE-:[490..490] *)) (VP (VBG:[491..500] examining) (NP (NN:[501..506] K-ras) (NN:[507..513] status)) (PP-LOC (IN:[514..516] in) (NP (NP (NP (NNS:[517..523] stools)) (CC:[524..527] and) (NP (NN:[528..534] tissue) (NNS:[535..542] samples))) (PP (IN:[543..547] from) (NP (NP (NNS:[548..556] patients)) (PP (IN:[558..562] with) (NP (NP (JJ:[563..573] pancreatic) (NNS:[574..580] tumors)) (CC:[581..584] and) (NP (JJ:[585..592] chronic) (NN:[593..605] pancreatitis))))))))))))) (.:[605..606] .))) ;sentence 5 Span:607..807 ;In tissue samples, K-ras mutations were found in 32 of 35 cases of ductal ;adenocarcinoma, in 5 of 7 periampullary cancers, in 1 cystadenocarcinoma, ;and in 3 of 5 patients with chronic pancreatitis. ;[626..631]:gene-rna:"K-ras" ;[675..696]:malignancy:"ductal adenocarcinoma" ;[709..730]:malignancy:"periampullary cancers" ;[737..755]:malignancy:"cystadenocarcinoma" ;[786..806]:malignancy:"chronic pancreatitis" (SENT (S (PP (IN:[607..609] In) (NP (NN:[610..616] tissue) (NNS:[617..624] samples))) (,:[624..625] ,) (NP-SBJ-1 (NN:[626..631] K-ras) (NNS:[633..642] mutations)) (VP (VBD:[643..647] were) (VP (VBN:[648..653] found) (NP-1 (-NONE-:[653..653] *)) (PP (PP (IN:[654..656] in) (NP (NP (CD:[657..659] 32)) (PP (IN:[660..662] of) (NP (NP (CD:[663..665] 35) (NNS:[666..671] cases)) (PP (IN:[672..674] of) (NP (JJ:[675..681] ductal) (NN:[682..696] adenocarcinoma))))))) (,:[696..697] ,) (PP (IN:[698..700] in) (NP (NP (CD:[701..702] 5)) (PP (IN:[703..705] of) (NP (CD:[706..707] 7) (JJ:[709..722] periampullary) (NNS:[723..730] cancers))))) (,:[730..731] ,) (PP-LOC (IN:[732..734] in) (NP (CD:[735..736] 1) (NN:[737..755] cystadenocarcinoma))) (,:[755..756] ,) (CC:[757..760] and) (PP-LOC (IN:[761..763] in) (NP (NP (CD:[764..765] 3)) (PP (IN:[766..768] of) (NP (NP (CD:[769..770] 5) (NNS:[771..779] patients)) (PP (IN:[780..784] with) (NP (JJ:[786..793] chronic) (NN:[794..806] pancreatitis)))))))))) (.:[806..807] .))) ;sentence 6 Span:808..965 ;In stools, mutated K-ras was seen in 10 of 25 cases of ductal ;adenocarcinoma, in 1 case of cystadenocarcinoma, and in 2 of 6 cases of ;chronic pancreatitis. ;[827..832]:gene-rna:"K-ras" ;[864..885]:malignancy:"ductal adenocarcinoma" ;[900..918]:malignancy:"cystadenocarcinoma" (SENT (S (PP-LOC (IN:[808..810] In) (NP (NNS:[811..817] stools))) (,:[817..818] ,) (NP-SBJ-1 (VBN:[819..826] mutated) (NN:[827..832] K-ras)) (VP (VBD:[833..836] was) (VP (VBN:[837..841] seen) (NP-1 (-NONE-:[841..841] *)) (PP (PP (IN:[842..844] in) (NP (NP (CD:[845..847] 10)) (PP (IN:[848..850] of) (NP (NP (CD:[851..853] 25) (NNS:[854..859] cases)) (PP (IN:[860..862] of) (NP (JJ:[864..870] ductal) (NN:[871..885] adenocarcinoma))))))) (,:[885..886] ,) (PP (IN:[887..889] in) (NP (NP (CD:[890..891] 1) (NN:[892..896] case)) (PP (IN:[897..899] of) (NP (NN:[900..918] cystadenocarcinoma))))) (,:[918..919] ,) (CC:[920..923] and) (PP (IN:[924..926] in) (NP (NP (CD:[927..928] 2)) (PP (IN:[929..931] of) (NP (NP (CD:[932..933] 6) (NNS:[934..939] cases)) (PP (IN:[940..942] of) (NP (JJ:[944..951] chronic) (NN:[952..964] pancreatitis)))))))))) (.:[964..965] .))) ;sentence 7 Span:966..1193 ;These data indicate that the K-ras status of stool samples may help identify ;pancreatic carcinoma and persons at risk for cancer development; however, it ;does not allow discrimination of malignant from nonmalignant diseases. ;[995..1000]:gene-rna:"K-ras" ;[1044..1064]:malignancy:"pancreatic carcinoma" (SENT (S (NP-SBJ (DT:[966..971] These) (NNS:[972..976] data)) (VP (VBP:[977..985] indicate) (SBAR (IN:[986..990] that) (S (NP-SBJ (NP (DT:[991..994] the) (NN:[995..1000] K-ras) (NN:[1001..1007] status)) (PP (IN:[1008..1010] of) (NP (NN:[1011..1016] stool) (NNS:[1017..1024] samples)))) (VP (MD:[1026..1029] may) (VP (VB:[1030..1034] help) (S-ADV (NP-SBJ (-NONE-:[1034..1034] *)) (VP (VB:[1035..1043] identify) (NP (NP (JJ:[1044..1054] pancreatic) (NN:[1055..1064] carcinoma)) (CC:[1065..1068] and) (NP (NP (NNS:[1069..1076] persons)) (PP (IN:[1077..1079] at) (NP (NP (NN:[1080..1084] risk)) (PP (IN:[1085..1088] for) (NP (NN:[1089..1095] cancer) (NN:[1097..1108] development))))))))))))) (,:[1108..1109] ;) (S-ADV (ADVP (RB:[1110..1117] however)) (,:[1117..1118] ,) (NP-SBJ (PRP:[1119..1121] it)) (VP (VBZ:[1122..1126] does) (RB:[1127..1130] not) (VP (VB:[1131..1136] allow) (NP (NP (NN:[1137..1151] discrimination)) (PP (IN:[1152..1154] of) (NP (JJ:[1155..1164] malignant) (NML-1 (-NONE-:[1164..1164] *P*)))) (PP (IN:[1165..1169] from) (NP (JJ:[1171..1183] nonmalignant) (NML-1 (NNS:[1184..1192] diseases))))))))) (.:[1192..1193] .))) ;section 8 Span:1197..1241 ;PMID: 9761241 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1197..1201] PMID) (::[1201..1202] :) (CD:[1203..1210] 9761241) (IN:[1211..1212] -LSB-) (NNP:[1212..1218] PubMed) (HYPH:[1219..1220] -) (JJ:[1221..1228] indexed) (IN:[1229..1232] for) (NNP:[1233..1241] MEDLINE-RSB-)))