;PMID: 9799749 ;source_file_1389.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..33] = [t:0..33] ;1)sentence:[e:38..195] = [t:38..195] ;2)section:[e:199..306] = [t:199..306] ;3)section:[e:310..436] = [t:310..436] ;4)sentence:[e:440..523] = [t:440..523] ;5)sentence:[e:524..862] = [t:524..862] ;6)sentence:[e:863..919] = [t:863..919] ;7)sentence:[e:920..1069] = [t:920..1069] ;8)sentence:[e:1070..1177] = [t:1070..1177] ;9)sentence:[e:1178..1252] = [t:1178..1252] ;10)sentence:[e:1253..1413] = [t:1253..1413] ;11)section:[e:1417..1461] = [t:1417..1461] ;section 0 Span:0..33 ;Clin Chem 1998 Nov;44(11):2243-8 (SEC (FRAG (DT:[0..4] Clin) (JJ:[5..9] Chem) (CD:[11..15] 1998) (CD:[16..23] Nov;44-LRB-) (CD:[23..25] 11) (-RRB-:[25..26] -RRB-) (CD:[26..31] :2243) (::[31..32] -) (CD:[32..33] 8))) ;sentence 1 Span:38..195 ;K-ras gene mutations in the diagnosis of fine-needle aspirates of pancreatic ;masses: prospective study using two techniques with different detection ;limits. ;[38..43]:gene-rna:"K-ras" ;[104..122]:malignancy:"pancreatic masses" (SENT (NP-HLN (NP (NP (NN:[38..43] K-ras) (NN:[44..48] gene) (NNS:[49..58] mutations)) (PP (IN:[59..61] in) (NP (NP (DT:[62..65] the) (NN:[66..75] diagnosis)) (PP (IN:[76..78] of) (NP (NP (NML (JJ:[79..83] fine) (HYPH:[83..84] -) (NN:[84..90] needle)) (NNS:[91..100] aspirates)) (PP (IN:[101..103] of) (NP (JJ:[104..114] pancreatic) (NNS:[116..122] masses)))))))) (::[122..123] :) (NP (NP (JJ:[124..135] prospective) (NN:[136..141] study)) (VP (VBG:[142..147] using) (NP (NP (CD:[148..151] two) (NNS:[152..162] techniques)) (PP (IN:[163..167] with) (NP (JJ:[168..177] different) (NN:[178..187] detection) (NNS:[188..194] limits)))))) (.:[194..195] .))) ;section 2 Span:199..306 ;Mora J, Puig P, Boadas J, Urgell E, Montserrat E, Lerma E, Gonzalez-Sastre F, ; Lluis F, Farre A, Capella G. (SEC (FRAG (NNP:[199..203] Mora) (NNP:[204..206] J,) (NNP:[207..211] Puig) (NNP:[212..213] P) (,:[213..214] ,) (NNP:[215..221] Boadas) (NNP:[222..224] J,) (NNP:[225..231] Urgell) (NNP:[232..233] E) (,:[233..234] ,) (NNP:[235..245] Montserrat) (NNP:[246..247] E) (,:[247..248] ,) (NNP:[249..254] Lerma) (NNP:[255..256] E) (,:[256..257] ,) (NNP:[258..266] Gonzalez) (HYPH:[266..267] -) (JJ:[267..273] Sastre) (NNP:[274..275] F) (,:[275..276] ,) (DT:[278..283] Lluis) (NNP:[284..285] F) (,:[285..286] ,) (DT:[287..292] Farre) (NNP:[293..294] A) (,:[294..295] ,) (NNP:[296..303] Capella) (NNP:[304..306] G.))) ;section 3 Span:310..436 ;Department of Clinical Biochemistry, Hospital de la Santa Creu i Sant Pau, ;Antoni M. Claret, Barcelona, Spain. lig@santpau.es (SEC (FRAG (NNP:[310..320] Department) (IN:[321..323] of) (NNP:[324..332] Clinical) (NNP:[333..345] Biochemistry) (NNP:[345..346] ,) (NNP:[347..355] Hospital) (IN:[356..358] de) (DT:[359..361] la) (NNP:[362..367] Santa) (NNP:[368..372] Creu) (NNP:[373..374] i) (NNP:[375..379] Sant) (NNP:[380..383] Pau) (,:[383..384] ,) (NNP:[386..392] Antoni) (NNP:[393..395] M.) (NNP:[396..402] Claret) (,:[402..403] ,) (NNP:[404..413] Barcelona) (,:[413..414] ,) (NNP:[415..420] Spain) (.:[420..421] .) ('':[422..436] lig@santpau.es))) ;sentence 4 Span:440..523 ;Detection of K-ras mutations may be useful in the evaluation of pancreatic ;cancer. ;[453..458]:gene-rna:"K-ras" ;[504..522]:malignancy:"pancreatic cancer" (SENT (S (NP-SBJ (NP (NN:[440..449] Detection)) (PP (IN:[450..452] of) (NP (NN:[453..458] K-ras) (NNS:[459..468] mutations)))) (VP (MD:[469..472] may) (VP (VB:[473..475] be) (ADJP-PRD (JJ:[476..482] useful) (PP (IN:[483..485] in) (NP (NP (DT:[486..489] the) (NN:[490..500] evaluation)) (PP (IN:[501..503] of) (NP (JJ:[504..514] pancreatic) (NN:[516..522] cancer)))))))) (.:[522..523] .))) ;sentence 5 Span:524..862 ;The aim of this study was to assess, in a prospective design, the diagnostic ;utility of K-ras mutation analysis in 62 consecutive fine-needle aspirates ;of pancreatic masses, using two PCR-based techniques-standard and ;enriched-with detection limits of a mutant allele in the presence of 10(2) or ; 10(3) wild-type alleles, respectively. ;[613..618]:gene-rna:"K-ras" ;[681..698]:malignancy:"pancreatic masses" (SENT (S (NP-SBJ (NP (DT:[524..527] The) (NN:[528..531] aim)) (PP (IN:[532..534] of) (NP (DT:[535..539] this) (NN:[540..545] study)))) (VP (VBD:[546..549] was) (S-NOM-PRD (NP-SBJ (-NONE-:[549..549] *)) (VP (TO:[550..552] to) (VP (VB:[553..559] assess) (,:[559..560] ,) (PP (IN:[561..563] in) (NP (DT:[564..565] a) (JJ:[566..577] prospective) (NN:[578..584] design))) (,:[584..585] ,) (NP (NP (DT:[586..589] the) (JJ:[591..601] diagnostic) (NN:[602..609] utility)) (PP (IN:[610..612] of) (NP (NN:[613..618] K-ras) (NN:[619..627] mutation) (NN:[628..636] analysis)))) (PP (IN:[637..639] in) (NP (NP (CD:[640..642] 62) (JJ:[643..654] consecutive) (NML (JJ:[655..659] fine) (HYPH:[659..660] -) (NN:[660..666] needle)) (NNS:[668..677] aspirates)) (PP (IN:[678..680] of) (NP (JJ:[681..691] pancreatic) (NNS:[692..698] masses))))) (,:[698..699] ,) (S-MNR (NP-SBJ (-NONE-:[699..699] *)) (VP (VBG:[700..705] using) (NP (NP (CD:[706..709] two) (ADJP (NN:[710..713] PCR) (HYPH:[713..714] -) (VBN:[714..719] based)) (NNS:[720..730] techniques)) (::[730..731] -) (NP (JJ:[731..739] standard) (CC:[740..743] and) (VBN:[745..753] enriched))) (::[753..754] -) (PP (IN:[754..758] with) (NP (NP (NN:[759..768] detection) (NNS:[769..775] limits)) (PP (IN:[776..778] of) (NP (NP (DT:[779..780] a) (JJ:[781..787] mutant) (NN:[788..794] allele)) (PP (IN:[795..797] in) (NP (NP (DT:[798..801] the) (NN:[802..810] presence)) (PP (IN:[811..813] of) (NP (NP (CD:[814..819] 10-LRB-2-RRB-) (NML-1 (-NONE-:[819..819] *P*))) (CC:[820..822] or) (NP (CD:[824..829] 10-LRB-3-RRB-) (NML-1 (JJ:[830..834] wild) (HYPH:[834..835] -) (NN:[835..839] type) (NNS:[840..847] alleles))))))))))) (,:[847..848] ,) (ADVP (RB:[849..861] respectively)))))))) (.:[861..862] .))) ;sentence 6 Span:863..919 ;Cytology alone offered a diagnostic sensitivity of 75%. (SENT (S (NP-SBJ (NP (NN:[863..871] Cytology)) (ADVP (RB:[872..877] alone))) (VP (VBD:[878..885] offered) (NP (NP (DT:[886..887] a) (JJ:[888..898] diagnostic) (NN:[900..911] sensitivity)) (PP (IN:[912..914] of) (NP (CD:[915..917] 75) (NN:[917..918] %))))) (.:[918..919] .))) ;sentence 7 Span:920..1069 ;The enriched higher sensitivity detection technique, in combination with ;cytology, offered a diagnostic sensitivity of 91% without false positives. (SENT (S (NP-SBJ (NP (DT:[920..923] The) (VBN:[924..932] enriched) (ADJP (JJR:[933..939] higher) (NN:[940..951] sensitivity)) (NN:[952..961] detection) (NN:[962..971] technique)) (,:[971..972] ,) (PP (IN:[973..975] in) (NP (NP (NN:[977..988] combination)) (PP (IN:[989..993] with) (NP (NN:[994..1002] cytology)))))) (,:[1002..1003] ,) (VP (VBD:[1004..1011] offered) (NP (NP (DT:[1012..1013] a) (JJ:[1014..1024] diagnostic) (NN:[1025..1036] sensitivity)) (PP (IN:[1037..1039] of) (NP (CD:[1040..1042] 91) (NN:[1042..1043] %))) (PP (IN:[1044..1051] without) (NP (JJ:[1052..1057] false) (NNS:[1059..1068] positives))))) (.:[1068..1069] .))) ;sentence 8 Span:1070..1177 ;The molecular analysis would have contributed to diagnosis in an additional ;14 cases of pancreatic cancer. ;[1159..1176]:malignancy:"pancreatic cancer" (SENT (S (NP-SBJ (DT:[1070..1073] The) (JJ:[1074..1083] molecular) (NN:[1084..1092] analysis)) (VP (MD:[1093..1098] would) (VP (VB:[1099..1103] have) (VP (VBN:[1104..1115] contributed) (PP (TO:[1116..1118] to) (NP (NN:[1119..1128] diagnosis))) (PP (IN:[1129..1131] in) (NP (NP (DT:[1132..1134] an) (JJ:[1136..1146] additional) (CD:[1147..1149] 14) (NNS:[1150..1155] cases)) (PP (IN:[1156..1158] of) (NP (JJ:[1159..1169] pancreatic) (NN:[1170..1176] cancer)))))))) (.:[1176..1177] .))) ;sentence 9 Span:1178..1252 ;The standard technique contributed to diagnosis in an additional 9 cases. (SENT (S (NP-SBJ (DT:[1178..1181] The) (JJ:[1182..1190] standard) (NN:[1191..1200] technique)) (VP (VBD:[1201..1212] contributed) (PP (TO:[1213..1215] to) (NP (NN:[1217..1226] diagnosis))) (PP (IN:[1227..1229] in) (NP (DT:[1230..1232] an) (JJ:[1233..1243] additional) (CD:[1244..1245] 9) (NNS:[1246..1251] cases)))) (.:[1251..1252] .))) ;sentence 10 Span:1253..1413 ;These results strongly support the use of the enriched method of detecting ;K-ras mutations as a complement to cytology in the evaluation of pancreatic ;masses. ;[1329..1334]:gene-rna:"K-ras" ;[1395..1412]:malignancy:"pancreatic masses" (SENT (S (NP-SBJ (DT:[1253..1258] These) (NNS:[1259..1266] results)) (ADVP (RB:[1267..1275] strongly)) (VP (VBP:[1276..1283] support) (NP (NP (DT:[1284..1287] the) (NN:[1288..1291] use)) (PP (IN:[1292..1294] of) (NP (NP (DT:[1296..1299] the) (VBN:[1300..1308] enriched) (NN:[1309..1315] method)) (PP (IN:[1316..1318] of) (S-NOM (NP-SBJ (-NONE-:[1318..1318] *)) (VP (VBG:[1319..1328] detecting) (NP (NN:[1329..1334] K-ras) (NNS:[1335..1344] mutations))))))) (PP (IN:[1345..1347] as) (NP (NP (DT:[1348..1349] a) (NN:[1350..1360] complement)) (PP (TO:[1361..1363] to) (NP (NN:[1364..1372] cytology)))))) (PP (IN:[1373..1375] in) (NP (NP (DT:[1377..1380] the) (NN:[1381..1391] evaluation)) (PP (IN:[1392..1394] of) (NP (JJ:[1395..1405] pancreatic) (NNS:[1406..1412] masses)))))) (.:[1412..1413] .))) ;section 11 Span:1417..1461 ;PMID: 9799749 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1417..1421] PMID) (::[1421..1422] :) (CD:[1423..1430] 9799749) (IN:[1431..1432] -LSB-) (NNP:[1432..1438] PubMed) (HYPH:[1439..1440] -) (JJ:[1441..1448] indexed) (IN:[1449..1452] for) (NNP:[1453..1461] MEDLINE-RSB-)))