;PMID: 3038106 ;source_file_1237.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..53] = [t:0..53] ;1)sentence:[e:58..135] = [t:58..135] ;2)section:[e:139..181] = [t:139..181] ;3)sentence:[e:185..423] = [t:185..423] ;4)sentence:[e:424..623] = [t:424..623] ;5)sentence:[e:624..711] = [t:624..711] ;6)section:[e:715..759] = [t:715..759] ;section 0 Span:0..53 ;Biochem Biophys Res Commun 1987 Jul 15;146(1):247-52 (SEC (FRAG (NNP:[0..7] Biochem) (NNP:[8..15] Biophys) (NNP:[16..19] Res) (NNP:[20..26] Commun) (CD:[28..32] 1987) (NNP:[33..36] Jul) (CD:[37..39] 15) (CD:[39..44] ;146-LRB-) (CD:[44..45] 1) (-RRB-:[45..46] -RRB-) (CD:[46..50] :247) (::[50..51] -) (CD:[51..53] 52))) ;sentence 1 Span:58..135 ;A point mutation at codon 13 of the N-ras oncogene in a human stomach ;cancer. ;[60..74]:variation-type:"point mutation" ;[78..86]:variation-location:"codon 13" ;[94..99]:gene-rna:"N-ras" ;[120..134]:malignancy:"stomach cancer" (SENT (NP-HLN (NP (DT:[58..59] A) (NN:[60..65] point) (NN:[66..74] mutation)) (PP-LOC (IN:[75..77] at) (NP (NP (NN:[78..83] codon) (CD:[84..86] 13)) (PP (IN:[87..89] of) (NP (DT:[90..93] the) (NN:[94..99] N-ras) (NN:[100..108] oncogene))))) (PP-LOC (IN:[109..111] in) (NP (DT:[112..113] a) (JJ:[114..119] human) (NN:[120..127] stomach) (NN:[128..134] cancer))) (.:[134..135] .))) ;section 2 Span:139..181 ;Nishida J, Kobayashi Y, Hirai H, Takaku F. (SEC (FRAG (NNP:[139..146] Nishida) (NNP:[147..149] J,) (NNP:[150..159] Kobayashi) (NNP:[160..161] Y) (,:[161..162] ,) (NNP:[163..168] Hirai) (NNP:[169..170] H) (,:[170..171] ,) (NNP:[172..178] Takaku) (NNP:[179..181] F.))) ;sentence 3 Span:185..423 ;A surgically removed human stomach cancer with the histological diagnosis of ;poorly differentiated adenocarcinoma contained an activated N-ras oncogene ;detected by an in vivo selection assay in nude mice using transfected NIH3T3 ;cells. ;[212..226]:malignancy:"stomach cancer" ;[263..299]:malignancy:"poorly differentiated adenocarcinoma" ;[323..328]:gene-rna:"N-ras" (SENT (S (NP-SBJ (NP (DT:[185..186] A) (ADJP (RB:[187..197] surgically) (VBN:[198..205] removed)) (JJ:[206..211] human) (NN:[212..219] stomach) (NN:[220..226] cancer)) (PP (IN:[227..231] with) (NP (NP (DT:[232..235] the) (JJ:[236..248] histological) (NN:[249..258] diagnosis)) (PP (IN:[259..261] of) (NP (ADJP (RB:[263..269] poorly) (VBN:[270..284] differentiated)) (NN:[285..299] adenocarcinoma)))))) (VP (VBD:[300..309] contained) (NP (NP (DT:[310..312] an) (VBN:[313..322] activated) (NN:[323..328] N-ras) (NN:[329..337] oncogene)) (VP (VBN:[339..347] detected) (NP (-NONE-:[347..347] *)) (PP-MNR (IN:[348..350] by) (NP (DT:[351..353] an) (ADJP (FW:[354..356] in) (FW:[357..361] vivo)) (NN:[362..371] selection) (NN:[372..377] assay))) (PP (IN:[378..380] in) (NP (JJ:[381..385] nude) (NNS:[386..390] mice))) (S-ADV (NP-SBJ (-NONE-:[390..390] *)) (VP (VBG:[391..396] using) (NP (VBN:[397..408] transfected) (NN:[409..415] NIH3T3) (NNS:[417..422] cells))))))) (.:[422..423] .))) ;sentence 4 Span:424..623 ;Analysis using synthetic 20-mer oligonucleotide probes revealed a point ;mutation from G to C at the first letter of codon 13 of the N-ras gene ;resulting in the substitution of arginine for glycine. ;[490..505]:variation-type:"point mutation" ;[511..512]:variation-state-original:"G" ;[516..517]:variation-state-altered:"C" ;[525..549]:variation-location:"first letter of codon 13" ;[557..562]:gene-rna:"N-ras" ;[586..598]:variation-type:"substitution" ;[602..610]:variation-state-altered:"arginine" ;[615..622]:variation-state-original:"glycine" (SENT (S (NP-SBJ (NP (NN:[424..432] Analysis)) (VP (VBG:[433..438] using) (NP (JJ:[439..448] synthetic) (NML (CD:[449..451] 20) (HYPH:[451..452] -) (NN:[452..455] mer)) (NN:[456..471] oligonucleotide) (NNS:[472..478] probes)))) (VP (VBD:[479..487] revealed) (NP (NP (DT:[488..489] a) (NN:[490..495] point) (NN:[497..505] mutation)) (PP (IN:[506..510] from) (NP (NN:[511..512] G))) (PP (TO:[513..515] to) (NP (NN:[516..517] C)))) (PP-LOC (IN:[518..520] at) (NP (NP (DT:[521..524] the) (JJ:[525..530] first) (NN:[531..537] letter)) (PP (IN:[538..540] of) (NP (NP (NN:[541..546] codon) (CD:[547..549] 13)) (PP (IN:[550..552] of) (NP (DT:[553..556] the) (NN:[557..562] N-ras) (NN:[563..567] gene))))))) (S-ADV (NP-SBJ (-NONE-:[567..567] *)) (VP (VBG:[568..577] resulting) (PP-CLR (IN:[579..581] in) (NP (NP (DT:[582..585] the) (NN:[586..598] substitution)) (PP (IN:[599..601] of) (NP (NN:[602..610] arginine))) (PP (IN:[611..614] for) (NP (NN:[615..622] glycine)))))))) (.:[622..623] .))) ;sentence 5 Span:624..711 ;This is the first observation of an activated N-ras oncogene in human ;stomach cancers. ;[671..676]:gene-rna:"N-ras" ;[695..710]:malignancy:"stomach cancers" (SENT (S (NP-SBJ (DT:[624..628] This)) (VP (VBZ:[629..631] is) (NP-PRD (NP (DT:[632..635] the) (JJ:[636..641] first) (NN:[642..653] observation)) (PP (IN:[654..656] of) (NP (DT:[657..659] an) (VBN:[661..670] activated) (NN:[671..676] N-ras) (NN:[677..685] oncogene))) (PP (IN:[686..688] in) (NP (JJ:[689..694] human) (NN:[695..702] stomach) (NNS:[703..710] cancers))))) (.:[710..711] .))) ;section 6 Span:715..759 ;PMID: 3038106 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[715..719] PMID) (::[719..720] :) (CD:[721..728] 3038106) (NN:[729..730] -LSB-) (NNP:[730..736] PubMed) (::[737..738] -) (NN:[739..746] indexed) (IN:[747..750] for) (NNP:[751..759] MEDLINE-RSB-)))