;PMID: 10402253 ;source_file_1214.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..33] = [t:0..33] ;1)sentence:[e:38..159] = [t:38..159] ;2)section:[e:163..211] = [t:163..211] ;3)section:[e:215..310] = [t:215..310] ;4)sentence:[e:314..485] = [t:314..485] ;5)sentence:[e:486..631] = [t:486..631] ;6)sentence:[e:633..879] = [t:633..879] ;7)sentence:[e:880..1095] = [t:880..1095] ;8)section:[e:1099..1144] = [t:1099..1144] ;section 0 Span:0..33 ;Int J Oncol 1999 Aug;15(2):391-8 (SEC (FRAG (NNP:[0..3] Int) (NNP:[4..5] J) (NNP:[6..11] Oncol) (CD:[13..17] 1999) (.:[18..25] Aug;15-LRB-) (CD:[25..26] 2) (-RRB-:[26..27] -RRB-) (CD:[27..31] :391) (::[31..32] -) (CD:[32..33] 8))) ;sentence 1 Span:38..159 ;Sampling technique influences the detection of K-ras mutations in normal ;appearing mucosa of colorectal cancer patients. ;[85..90]:gene-rna:"K-ras" ;[132..149]:malignancy:"colorectal cancer" (SENT (S (S-NOM-SBJ (NP-SBJ (-NONE-:[38..38] *)) (VP (VBG:[38..46] Sampling) (NP (NN:[47..56] technique)))) (VP (VBZ:[57..67] influences) (NP (NP (DT:[68..71] the) (NN:[72..81] detection)) (PP (IN:[82..84] of) (NP (NN:[85..90] K-ras) (NNS:[91..100] mutations))) (PP-LOC (IN:[101..103] in) (NP (NP (ADJP (JJ:[104..110] normal) (VBG:[112..121] appearing)) (NN:[122..128] mucosa)) (PP (IN:[129..131] of) (NP (NML (JJ:[132..142] colorectal) (NN:[143..149] cancer)) (NNS:[150..158] patients))))))) (.:[158..159] .))) ;section 2 Span:163..211 ;Schimanski CC, Sutter C, Linnemann U, Berger MR. (SEC (FRAG (NNP:[163..173] Schimanski) (NNP:[174..176] CC) (,:[176..177] ,) (NNP:[178..184] Sutter) (NNP:[185..186] C) (,:[186..187] ,) (NNP:[188..197] Linnemann) (NNP:[198..199] U) (,:[199..200] ,) (NNP:[201..207] Berger) (NNP:[208..210] MR) (.:[210..211] .))) ;section 3 Span:215..310 ;Unit of Toxicology and Chemotherapy, German Cancer Research Centre, 69120 ;Heidelberg, Germany. (SEC (FRAG (NNP:[215..219] Unit) (IN:[220..222] of) (NNP:[223..233] Toxicology) (CC:[234..237] and) (NNP:[238..250] Chemotherapy) (,:[250..251] ,) (NNP:[252..258] German) (NNP:[259..265] Cancer) (NNP:[266..274] Research) (NNP:[275..281] Centre) (,:[281..282] ,) (CD:[283..288] 69120) (NNP:[290..300] Heidelberg) (,:[300..301] ,) (NNP:[302..309] Germany) (.:[309..310] .))) ;sentence 4 Span:314..485 ;Based on three colorectal cancer cell lines with specified K-ras status, a ;sensitive PCR-RFLP assay was established detecting one K-ras mutant among 106 ; wild-type cells. ;[329..357]:malignancy:"colorectal cancer cell lines" ;[373..378]:gene-rna:"K-ras" ;[445..450]:gene-rna:"K-ras" (SENT (S (PP (VBN:[314..319] Based) (PP (IN:[320..322] on) (NP (NP (CD:[323..328] three) (JJ:[329..339] colorectal) (NN:[340..346] cancer) (NN:[347..351] cell) (NN:[352..357] lines)) (PP (IN:[358..362] with) (NP (VBN:[363..372] specified) (NN:[373..378] K-ras) (NN:[379..385] status)))))) (,:[385..386] ,) (NP-SBJ-1 (DT:[387..388] a) (JJ:[390..399] sensitive) (NML (NN:[400..403] PCR) (HYPH:[403..404] -) (NN:[404..408] RFLP)) (NN:[409..414] assay)) (VP (VBD:[415..418] was) (VP (VBN:[419..430] established) (NP-1 (-NONE-:[430..430] *)) (S-ADV (NP-SBJ (-NONE-:[430..430] *)) (VP (VBG:[431..440] detecting) (NP (NP (CD:[441..444] one) (NN:[445..450] K-ras) (NN:[451..457] mutant)) (PP (IN:[458..463] among) (NP (CD:[464..467] 106) (NML (JJ:[469..473] wild) (HYPH:[473..474] -) (NN:[474..478] type)) (NNS:[479..484] cells)))))))) (.:[484..485] .))) ;sentence 5 Span:486..631 ;Using this assay for tissues of 124 colorectal cancer patients, 59 tumor ;(46%) and 11 mucosa samples (9%) were found to harbor a K-ras mutation. ;[522..539]:malignancy:"colorectal cancer" ;[554..559]:malignancy:"tumor" ;[616..621]:gene-rna:"K-ras" (SENT (S (S-MNR (NP-SBJ (-NONE-:[486..486] *)) (VP (VBG:[486..491] Using) (NP (NP (DT:[492..496] this) (NN:[497..502] assay)) (PP (IN:[503..506] for) (NP (NP (NNS:[507..514] tissues)) (PP (IN:[515..517] of) (NP (CD:[518..521] 124) (NML (JJ:[522..532] colorectal) (NN:[533..539] cancer)) (NNS:[540..548] patients)))))))) (,:[548..549] ,) (NP-SBJ-1 (NP (CD:[551..553] 59) (NN:[554..559] tumor) (PRN (-LRB-:[560..561] -LRB-) (NP (CD:[561..563] 46) (NN:[563..564] %)) (-RRB-:[564..565] -RRB-))) (CC:[566..569] and) (NP (CD:[570..572] 11) (NN:[573..579] mucosa) (NNS:[580..587] samples) (PRN (-LRB-:[588..589] -LRB-) (NP (CD:[589..590] 9) (NN:[590..591] %)) (-RRB-:[591..592] -RRB-)))) (VP (VBD:[593..597] were) (VP (VBN:[598..603] found) (S (NP-SBJ-1 (-NONE-:[603..603] *)) (VP (TO:[604..606] to) (VP (VB:[607..613] harbor) (NP (DT:[614..615] a) (NN:[616..621] K-ras) (NN:[622..630] mutation))))))) (.:[630..631] .))) ;sentence 6 Span:633..879 ;When using the same scalpel for collecting tumor and mucosa tissues (group ;A), 18% of the patients had a matching K-ras mutation in both tissues, but ;this coincidence was seen in 3% of patients only, when separate scalpels ;were used (group B). ;[676..681]...[693..700]:malignancy:"tumor"..."tissues" ;[748..753]:gene-rna:"K-ras" (SENT (S (S (SBAR (WHADVP-2 (WRB:[633..637] When)) (S (NP-SBJ (-NONE-:[637..637] *)) (VP (VBG:[638..643] using) (NP (DT:[644..647] the) (JJ:[648..652] same) (NN:[653..660] scalpel)) (PP-PRP (IN:[661..664] for) (S-NOM (NP-SBJ (-NONE-:[664..664] *)) (VP (VBG:[665..675] collecting) (NP (NP (NN:[676..681] tumor) (NML-1 (-NONE-:[681..681] *P*))) (CC:[682..685] and) (NP (JJ:[686..692] mucosa) (NML-1 (NNS:[693..700] tissues))))))) (PRN (-LRB-:[701..702] -LRB-) (NP (NN:[702..707] group) (NN:[708..709] A)) (-RRB-:[709..710] -RRB-)) (ADVP-TMP-2 (-NONE-:[710..710] *T*))))) (,:[710..711] ,) (NP-SBJ (NP (CD:[713..715] 18) (NN:[715..716] %)) (PP (IN:[717..719] of) (NP (DT:[720..723] the) (NNS:[724..732] patients)))) (VP (VBD:[733..736] had) (NP (DT:[737..738] a) (VBG:[739..747] matching) (NN:[748..753] K-ras) (NN:[754..762] mutation)) (PP-LOC (IN:[763..765] in) (NP (DT:[766..770] both) (NNS:[771..778] tissues))))) (,:[778..779] ,) (CC:[780..783] but) (S (NP-SBJ-3 (DT:[784..788] this) (NN:[790..801] coincidence)) (VP (VBD:[802..805] was) (VP (VBN:[806..810] seen) (NP-3 (-NONE-:[810..810] *)) (PP (IN:[811..813] in) (NP (NP (CD:[814..815] 3) (NN:[815..816] %)) (PP (IN:[817..819] of) (NP (NNS:[820..828] patients))) (ADVP (RB:[829..833] only)))) (,:[833..834] ,) (SBAR-ADV (WHADVP-5 (WRB:[835..839] when)) (S (NP-SBJ-4 (JJ:[840..848] separate) (NNS:[849..857] scalpels)) (VP (VBD:[858..862] were) (VP (VBN:[863..867] used) (NP-4 (-NONE-:[867..867] *)) (PRN (-LRB-:[869..870] -LRB-) (NP (NN:[870..875] group) (NN:[876..877] B)) (-RRB-:[877..878] -RRB-)) (ADVP-TMP-5 (-NONE-:[878..878] *T*))))))))) (.:[878..879] .))) ;sentence 7 Span:880..1095 ;Thus we conclude that the sampling technique used for collecting specimens ;is a major contributor to the detection of K-ras mutations in normal ;appearing mucosa when a highly sensitive detection technique is used. ;[999..1004]:gene-rna:"K-ras" (SENT (S (ADVP (RB:[880..884] Thus)) (NP-SBJ (PRP:[885..887] we)) (VP (VBP:[888..896] conclude) (SBAR (IN:[897..901] that) (S (NP-SBJ (NP (DT:[902..905] the) (VBG:[906..914] sampling) (NN:[915..924] technique)) (VP (VBN:[925..929] used) (NP (-NONE-:[929..929] *)) (PP (IN:[930..933] for) (S-NOM (NP-SBJ (-NONE-:[933..933] *)) (VP (VBG:[934..944] collecting) (NP (NNS:[946..955] specimens))))))) (VP (VBZ:[956..958] is) (NP-PRD (NP (DT:[959..960] a) (JJ:[961..966] major) (NN:[967..978] contributor)) (PP (TO:[979..981] to) (NP (NP (DT:[982..985] the) (NN:[986..995] detection)) (PP (IN:[996..998] of) (NP (NN:[999..1004] K-ras) (NNS:[1005..1014] mutations))) (PP (IN:[1015..1017] in) (NP (ADJP (JJ:[1018..1024] normal) (VBG:[1026..1035] appearing)) (NN:[1036..1042] mucosa)))))) (SBAR (WHADVP-2 (WRB:[1043..1047] when)) (S (NP-SBJ-1 (DT:[1048..1049] a) (ADJP (RB:[1050..1056] highly) (JJ:[1057..1066] sensitive)) (NN:[1067..1076] detection) (NN:[1077..1086] technique)) (VP (VBZ:[1087..1089] is) (VP (VBN:[1090..1094] used) (NP-1 (-NONE-:[1094..1094] *)) (ADVP-TMP-2 (-NONE-:[1094..1094] *T*)))))))))) (.:[1094..1095] .))) ;section 8 Span:1099..1144 ;PMID: 10402253 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1099..1103] PMID) (::[1103..1104] :) (CD:[1105..1113] 10402253) (NN:[1114..1115] -LSB-) (NNP:[1115..1121] PubMed) (::[1122..1123] -) (NN:[1124..1131] indexed) (IN:[1132..1135] for) (NNP:[1136..1144] MEDLINE-RSB-)))