Dig Dis Sci  2000 Oct;45(10):2022-8 

Combination analysis of genetic alterations and cell proliferation in small
intestinal carcinomas.

Muneyuki T, Watanabe M, Yamanaka M, Isaji S, Kawarada Y, Yatani R.

First Department of Surgery, Mie University School of Medicine, Tsu-shi, Japan.

We analyzed K-ras and p53 mutations, microsatellite instability (MSI), and loss
of heterozygosity (LOH) using the polymerase chain reaction (PCR) methods, as
well as p53 expression and the Ki-67 labeling index (LI) using
immunohistochemistry in 10 duodenal and 10 jejunal/ileal carcinomas. K-ras
mutations were detected in two duodenal (20%) and three jejunal/ileal (30%)
carcinomas and p53 mutations in one (10%) and three (30%), respectively. LOH of
17p was detected in two duodenal (20%) and two jejunal/ileal (20%) carcinomas
and p53 expression in four duodenal (40%) and four jejunal/ileal (40%). One
duodenal (10%) and two jejunal/ileal (20%) carcinomas demonstrated MSI. LOH of
APC was detected in three jejunal/ileal (30%), but none of the duodenal
carcinomas. The Ki-67 LI was 44% in duodenal and 52.6% in jejunal/ileal
carcinomas. A subset of small intestinal carcinomas showed involvement of K-ras
and p53 mutations, LOH of APC, and MSI. A difference was also apparent for LOH
of APC between duodenal and jejunal/ileal carcinomas.

PMID: 11117578 [PubMed - indexed for MEDLINE]