Nippon Shokakibyo Gakkai Zasshi  1989 Nov;86(11):2566-71 

[Detection of ras gene mutations of pancreatic tumors by polymerase chain
reaction and direct sequencing method]

[Article in Japanese]

Tada M, Yokosuka O, Omata M, Ohto M, Isono K.

Ras genes (H-,K-,N-ras) are converted to active oncogenes by point mutations
occurring in either codon 12, 13 or 61. We analyzed 19 pancreatic tumors
(formalin fixed paraffin embedded tissue) of these codons by a method to
directly sequence nucleotides around codons 12/13 and 61 of the three ras genes,
using polymerase chain reaction and direct sequencing method. Of 19 pancreatic
tumors, all 17 duct cell carcinomas involving 2 mucous producing pancreatic
cancers had point mutations of the K-ras codon 12, but 2 islet cell tumors had
ano point mutation around codons 12, 13, 61 of the three ras genes. Extremely
high incidence of ras gene mutation may be relevant to certain pathogenesis of
pancreatic cancers.

PMID: 2689731 [PubMed - indexed for MEDLINE]