Int J Hematol  1995 Aug;62(2):91-7 

N-ras and p53 gene mutations are very rare events in multiple myeloma.

Yasuga Y, Hirosawa S, Yamamoto K, Tomiyama J, Nagata K, Aokia N.

First Department of Internal Medicine, Tokyo Medical and Dental University,
Japan.

The frequencies of p53 and N-ras gene mutations were examined in multiple
myeloma. DNA samples of 45 cases of multiple myeloma were obtained from stored
bone marrow smears. All samples were analyzed for the N-ras gene at codon 12 by
the allele specific restriction analysis method and at codon 61 by direct
sequencing. DNA was screened for mutations by single-strand conformation
polymorphism analysis for the p53 gene in exons 5 to 8. DNA fragments showing an
altered electrophoretic pattern were further studied by direct sequencing to
confirm the mutations. Ras mutations were found in two cases at codon 61 (4.4%).
A point mutation of the p53 gene was detected in one of 45 cases (2.2%). These 3
cases with mutations were in an advanced stage. In conclusion, N-ras and p53
gene mutations may occur at a late stage of multiple myeloma, but the
frequencies of the mutations are very low.

PMID: 8590778 [PubMed - indexed for MEDLINE]