Jpn J Clin Oncol  2002 Jun;32(6):219-21 

Concurrent mutations of K-ras oncogene at codons 12 and 22 in colon cancer.

Miyakura Y, Sugano K, Fukayama N, Konishi F, Nagai H.

Department of Surgery, Jichi Medical School, Minami Kawachi, Tochigi, Japan.

K-ras mutation is the most common oncogenic alteration in various human cancers
including colorectal carcinomas. Point mutations have the potential to activate
the K-ras gene if they occur in the critical coding sequences. Almost all of
these mutations have been localized in codons 12, 13 and 61. We report a case of
colon cancer presenting point mutations at both codons 12 and 22 of the K-ras
gene. PCR-SSCP and subsequent sequencing revealed that GGT (glycine, wild-type)
to AGT (serine) substitution at codon 12 and CAG (glutamine, wild-type) to CGG
(arginine) substitution at codon 22 occurred in the same allele.

PMID: 12110640 [PubMed - indexed for MEDLINE]