;PMID: 8060487 ;source_file_1922.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..40] = [t:0..40] ;1)sentence:[e:46..223] = [t:46..223] ;2)section:[e:227..295] = [t:227..295] ;3)section:[e:299..382] = [t:299..382] ;4)sentence:[e:386..529] = [t:386..529] ;5)sentence:[e:530..724] = [t:530..724] ;6)sentence:[e:725..929] = [t:725..929] ;7)sentence:[e:931..1172] = [t:931..1172] ;8)sentence:[e:1174..1346] = [t:1174..1346] ;9)sentence:[e:1347..1410] = [t:1347..1410] ;10)sentence:[e:1412..1526] = [t:1412..1526] ;11)sentence:[e:1527..1684] = [t:1527..1684] ;12)sentence:[e:1685..1794] = [t:1685..1794] ;13)sentence:[e:1796..1960] = [t:1796..1960] ;14)sentence:[e:1961..2112] = [t:1961..2112] ;15)sentence:[e:2113..2244] = [t:2113..2244] ;16)sentence:[e:2245..2500] = [t:2245..2500] ;17)sentence:[e:2501..2690] = [t:2501..2690] ;18)section:[e:2694..2738] = [t:2694..2738] ;section 0 Span:0..40 ;J Mol Endocrinol. 1994 Apr;12(2):239-49. (SEC (FRAG (NNP:[0..1] J) (NNP:[2..5] Mol) (NNP:[6..16] Endocrinol) (.:[16..17] .) (CD:[18..22] 1994) (CC:[23..30] Apr;12-LRB-) (CD:[30..31] 2) (-RRB-:[31..32] -RRB-) (::[32..33] :) (CD:[33..36] 239) (CD:[36..39] -49) (.:[39..40] .))) ;sentence 1 Span:46..223 ;Differential regulation of cholesterol side-chain cleavage (P450scc) and ;aromatase (P450arom) enzyme mRNA expression by gonadotrophins and cyclic AMP ;in human granulosa cells. ;[73..104]:cyp450:"cholesterol side-chain cleavage" ;[106..113]:cyp450:"P450scc" ;[120..152]:substance:"aromatase (P450arom) enzyme mRNA" ;[167..181]:substance:"gonadotrophins" ;[186..196]:substance:"cyclic AMP" (SENT (NP-HLN (NP (JJ:[46..58] Differential) (NN:[59..69] regulation)) (PP (IN:[70..72] of) (NP (NP (NP (NN:[73..84] cholesterol) (NML (NN:[85..89] side) (HYPH:[89..90] -) (NN:[90..95] chain)) (NN:[96..104] cleavage)) (NP (-LRB-:[105..106] -LRB-) (NN:[106..113] P450scc) (-RRB-:[113..114] -RRB-))) (CC:[115..118] and) (NP (NML (NML (NN:[120..129] aromatase)) (NML (-LRB-:[130..131] -LRB-) (NN:[131..139] P450arom) (-RRB-:[139..140] -RRB-))) (NN:[141..147] enzyme) (NN:[148..152] mRNA) (NN:[153..163] expression)))) (PP (IN:[164..166] by) (NP (NP (NNS:[167..181] gonadotrophins)) (CC:[182..185] and) (NP (JJ:[186..192] cyclic) (NN:[193..196] AMP)))) (PP-LOC (IN:[197..199] in) (NP (JJ:[201..206] human) (NN:[207..216] granulosa) (NNS:[217..222] cells))) (.:[222..223] .))) ;section 2 Span:227..295 ;Yong EL, Hillier SG, Turner M, Baird DT, Ng SC, Bongso A, Ratnam SS. (SEC (FRAG (NNP:[227..231] Yong) (NNP:[232..234] EL) (,:[234..235] ,) (NNP:[236..243] Hillier) (NNP:[244..246] SG) (,:[246..247] ,) (NNP:[248..254] Turner) (NNP:[255..257] M,) (NNP:[258..263] Baird) (NNP:[264..266] DT) (,:[266..267] ,) (NNP:[268..270] Ng) (NNP:[271..273] SC) (,:[273..274] ,) (NNP:[275..281] Bongso) (NNP:[282..283] A) (,:[283..284] ,) (NNP:[285..291] Ratnam) (NNP:[292..294] SS) (.:[294..295] .))) ;section 3 Span:299..382 ;Department of Obstetrics and Gynaecology, National University Hospital, ;Singapore. (SEC (FRAG (NNP:[299..309] Department) (IN:[310..312] of) (NNP:[313..323] Obstetrics) (CC:[324..327] and) (NNP:[328..339] Gynaecology) (,:[339..340] ,) (NNP:[341..349] National) (NNP:[350..360] University) (NNP:[361..369] Hospital) (,:[369..370] ,) (NNP:[372..381] Singapore) (.:[381..382] .))) ;sentence 4 Span:386..529 ;The co-ordinated biosynthesis of progesterone and oestradiol in the human ;ovary is critical for reproductive cyclicity and eventual pregnancy. ;[419..431]:substance:"progesterone" ;[436..446]:substance:"oestradiol" (SENT (S (NP-SBJ (NP (DT:[386..389] The) (ADJP (AFX:[390..392] co) (HYPH:[392..393] -) (JJ:[393..402] ordinated)) (NN:[403..415] biosynthesis)) (PP (IN:[416..418] of) (NP (NN:[419..431] progesterone) (CC:[432..435] and) (NN:[436..446] oestradiol))) (PP-LOC (IN:[447..449] in) (NP (DT:[450..453] the) (JJ:[454..459] human) (NN:[460..465] ovary)))) (VP (VBZ:[467..469] is) (ADJP-PRD (JJ:[470..478] critical)) (PP (IN:[479..482] for) (NP (NP (JJ:[483..495] reproductive) (NN:[496..505] cyclicity)) (CC:[506..509] and) (NP (JJ:[510..518] eventual) (NN:[519..528] pregnancy))))) (.:[528..529] .))) ;sentence 5 Span:530..724 ;The crucial regulatory enzymes for progesterone and oestradiol biosynthesis ;in granulosa cells are the cholesterol side-chain cleavage (P450scc) and ;aromatase (P450arom) enzymes respectively. ;[554..561]:cyp450:"enzymes" ;[566..578]:substance:"progesterone" ;[583..593]:substance:"oestradiol" ;[635..676]...[703..710]:cyp450:"cholesterol side-chain cleavage ;(P450scc)"..."enzymes" ;[681..710]:cyp450:"aromatase (P450arom) enzymes" (SENT (S (NP-SBJ (NP (DT:[530..533] The) (JJ:[534..541] crucial) (JJ:[543..553] regulatory) (NNS:[554..561] enzymes)) (PP (IN:[562..565] for) (NP (NP (NN:[566..578] progesterone) (NML-2 (-NONE-:[578..578] *P*))) (CC:[579..582] and) (NP (NN:[583..593] oestradiol) (NML-2 (NN:[594..606] biosynthesis))))) (PP-LOC (IN:[607..609] in) (NP (NN:[610..619] granulosa) (NNS:[621..626] cells)))) (VP (VBP:[627..630] are) (NP-PRD (DT:[631..634] the) (NP (NML (NML (NML (NN:[635..646] cholesterol) (NML (NN:[647..651] side) (HYPH:[651..652] -) (NN:[652..657] chain)) (NN:[658..666] cleavage)) (NML (-LRB-:[667..668] -LRB-) (NN:[668..675] P450scc) (-RRB-:[675..676] -RRB-))) (NML-1 (-NONE-:[676..676] *P*))) (CC:[677..680] and) (NML (NML (NML (NN:[681..690] aromatase)) (NML (-LRB-:[691..692] -LRB-) (NN:[692..700] P450arom) (-RRB-:[700..701] -RRB-))) (NML-1 (NNS:[703..710] enzymes))))) (ADVP (RB:[711..723] respectively))) (.:[723..724] .))) ;sentence 6 Span:725..929 ;We utilized the cDNA sequences encoding P450arom and P450scc to examine the ;roles of FSH and LH, and their intracellular second messenger, cyclic AMP ;(cAMP), in regulating steroidogenic gene expression. ;[741..745]:substance:"cDNA" ;[765..773]:cyp450:"P450arom" ;[779..786]:cyp450:"P450scc" ;[811..814]:substance:"FSH" ;[819..821]:substance:"LH" ;[855..864]:substance:"messenger" ;[866..876]:substance:"cyclic AMP" ;[878..882]:substance:"cAMP" (SENT (S (NP-SBJ (PRP:[725..727] We)) (VP (VBD:[728..736] utilized) (NP (NP (DT:[737..740] the) (NN:[741..745] cDNA) (NNS:[746..755] sequences)) (VP (VBG:[756..764] encoding) (NP (NN:[765..773] P450arom) (CC:[774..777] and) (NN:[779..786] P450scc)))) (S-PRP (NP-SBJ (-NONE-:[786..786] *)) (VP (TO:[787..789] to) (VP (VB:[790..797] examine) (NP (NP (DT:[798..801] the) (NNS:[802..807] roles)) (PP (IN:[808..810] of) (NP (NP (NN:[811..814] FSH) (CC:[815..818] and) (NN:[819..821] LH)) (,:[821..822] ,) (CC:[823..826] and) (NP (NP (PRP$:[827..832] their) (JJ:[833..846] intracellular) (JJ:[847..853] second) (NN:[855..864] messenger)) (,:[864..865] ,) (NP (NP (JJ:[866..872] cyclic) (NN:[873..876] AMP)) (NP (-LRB-:[877..878] -LRB-) (NN:[878..882] cAMP) (-RRB-:[882..883] -RRB-)))))) (,:[883..884] ,) (PP (IN:[885..887] in) (S-NOM (NP-SBJ (-NONE-:[887..887] *)) (VP (VBG:[888..898] regulating) (NP (JJ:[899..912] steroidogenic) (NN:[913..917] gene) (NN:[918..928] expression)))))))))) (.:[928..929] .))) ;sentence 7 Span:931..1172 ;Mature granulosa cells (aspirated before the onset of the endogenous LH ;surge) and granulosa lutein cells (obtained after an ovulatory dose of human ;chorionic gonadotrophin) were cultured for 4 days with FSH, LH or dibutyryl ;cAMP (dbcAMP). ;[1000..1002]:substance:"LH" ;[1081..1105]:substance:"chorionic gonadotrophin" ;[1125..1126]:quantitative-value:"4" ;[1127..1131]:quantitative-units:"days" ;[1137..1140]:substance:"FSH" ;[1142..1144]:substance:"LH" ;[1148..1162]:substance:"dibutyryl cAMP" ;[1164..1170]:substance:"dbcAMP" (SENT (S (NP-SBJ-1 (NP (JJ:[931..937] Mature) (NN:[938..947] granulosa) (NNS:[948..953] cells) (PRN (-LRB-:[954..955] -LRB-) (VP (VBN:[955..964] aspirated) (NP (-NONE-:[964..964] *)) (PP-TMP (IN:[965..971] before) (NP (NP (DT:[972..975] the) (NN:[976..981] onset)) (PP (IN:[982..984] of) (NP (DT:[985..988] the) (JJ:[989..999] endogenous) (NN:[1000..1002] LH) (NN:[1003..1008] surge)))))) (-RRB-:[1008..1009] -RRB-))) (CC:[1011..1014] and) (NP (NN:[1015..1024] granulosa) (NN:[1025..1031] lutein) (NNS:[1032..1037] cells) (PRN (-LRB-:[1038..1039] -LRB-) (VP (VBN:[1039..1047] obtained) (NP (-NONE-:[1047..1047] *)) (PP (IN:[1048..1053] after) (NP (NP (DT:[1054..1056] an) (JJ:[1057..1066] ovulatory) (NN:[1067..1071] dose)) (PP (IN:[1072..1074] of) (NP (JJ:[1075..1080] human) (JJ:[1081..1090] chorionic) (NN:[1092..1105] gonadotrophin)))))) (-RRB-:[1105..1106] -RRB-)))) (VP (VBD:[1107..1111] were) (VP (VBN:[1112..1120] cultured) (NP-1 (-NONE-:[1120..1120] *)) (PP (IN:[1121..1124] for) (NP (CD:[1125..1126] 4) (NNS:[1127..1131] days))) (PP (IN:[1132..1136] with) (NP (NP (NN:[1137..1140] FSH)) (,:[1140..1141] ,) (NP (NN:[1142..1144] LH)) (CC:[1145..1147] or) (NP (NP (NN:[1148..1157] dibutyryl) (NN:[1158..1162] cAMP)) (NP (-LRB-:[1163..1164] -LRB-) (NN:[1164..1170] dbcAMP) (-RRB-:[1170..1171] -RRB-))))))) (.:[1171..1172] .))) ;sentence 8 Span:1174..1346 ;After the period of culture, total RNA was extracted from granulosa cells and ; Northern analyses were performed utilizing 32P-labelled cDNAs encoding ;P450arom and P450scc. ;[1209..1212]:substance:"RNA" ;[1296..1299]:substance:"32P" ;[1309..1314]:substance:"cDNAs" ;[1324..1332]:cyp450:"P450arom" ;[1338..1345]:cyp450:"P450scc" (SENT (S (S (PP-TMP (IN:[1174..1179] After) (NP (NP (DT:[1180..1183] the) (NN:[1184..1190] period)) (PP (IN:[1191..1193] of) (NP (NN:[1194..1201] culture))))) (,:[1201..1202] ,) (NP-SBJ-1 (JJ:[1203..1208] total) (NN:[1209..1212] RNA)) (VP (VBD:[1213..1216] was) (VP (VBN:[1217..1226] extracted) (NP-1 (-NONE-:[1226..1226] *)) (PP (IN:[1227..1231] from) (NP (NN:[1232..1241] granulosa) (NNS:[1242..1247] cells)))))) (CC:[1248..1251] and) (S (NP-SBJ-2 (JJ:[1253..1261] Northern) (NNS:[1262..1270] analyses)) (VP (VBD:[1271..1275] were) (VP (VBN:[1276..1285] performed) (NP-2 (-NONE-:[1285..1285] *)) (S-MNR (NP-SBJ (-NONE-:[1285..1285] *)) (VP (VBG:[1286..1295] utilizing) (NP (NP (ADJP (NN:[1296..1299] 32P) (HYPH:[1299..1300] -) (VBN:[1300..1308] labelled)) (NNS:[1309..1314] cDNAs)) (VP (VBG:[1315..1323] encoding) (NP (NN:[1324..1332] P450arom) (CC:[1334..1337] and) (NN:[1338..1345] P450scc))))))))) (.:[1345..1346] .))) ;sentence 9 Span:1347..1410 ;Spent culture media were analysed for steroid and cAMP content. ;[1385..1392]:substance:"steroid" ;[1397..1401]:substance:"cAMP" (SENT (S (NP-SBJ-2 (VBN:[1347..1352] Spent) (NN:[1353..1360] culture) (NNS:[1361..1366] media)) (VP (VBD:[1367..1371] were) (VP (VBN:[1372..1380] analysed) (NP-2 (-NONE-:[1380..1380] *)) (PP (IN:[1381..1384] for) (NP (NP (JJ:[1385..1392] steroid) (NML-1 (-NONE-:[1392..1392] *P*))) (CC:[1393..1396] and) (NP (NN:[1397..1401] cAMP) (NML-1 (NN:[1402..1409] content))))))) (.:[1409..1410] .))) ;sentence 10 Span:1412..1526 ;Both FSH and LH strongly stimulated P450arom mRNA expression and oestradiol ;production in mature granulosa cells. ;[1417..1420]:substance:"FSH" ;[1425..1427]:substance:"LH" ;[1448..1461]:substance:"P450arom mRNA" ;[1477..1487]:substance:"oestradiol" (SENT (S (NP-SBJ (CC:[1412..1416] Both) (NN:[1417..1420] FSH) (CC:[1421..1424] and) (NN:[1425..1427] LH)) (ADVP (RB:[1428..1436] strongly)) (VP (VBD:[1437..1447] stimulated) (NP (NP (NN:[1448..1456] P450arom) (NN:[1457..1461] mRNA) (NN:[1462..1472] expression)) (CC:[1473..1476] and) (NP (NN:[1477..1487] oestradiol) (NN:[1489..1499] production))) (PP-LOC (IN:[1500..1502] in) (NP (JJ:[1503..1509] mature) (NN:[1510..1519] granulosa) (NNS:[1520..1525] cells)))) (.:[1525..1526] .))) ;sentence 11 Span:1527..1684 ;On the other hand, P450scc mRNA expression and progesterone biosynthesis ;were weakly induced by FSH; maximal synthesis occurred only in the presence ;of LH. ;[1546..1558]:substance:"P450scc mRNA" ;[1575..1587]:substance:"progesterone" ;[1624..1627]:substance:"FSH" ;[1681..1683]:substance:"LH" (SENT (S (S (PP (IN:[1527..1529] On) (NP (DT:[1530..1533] the) (JJ:[1534..1539] other) (NN:[1540..1544] hand))) (,:[1544..1545] ,) (NP-SBJ-1 (NP (NN:[1546..1553] P450scc) (NN:[1554..1558] mRNA) (NN:[1559..1569] expression)) (CC:[1571..1574] and) (NP (NN:[1575..1587] progesterone) (NN:[1588..1600] biosynthesis))) (VP (VBD:[1601..1605] were) (ADVP (RB:[1606..1612] weakly)) (VP (VBN:[1613..1620] induced) (NP-1 (-NONE-:[1620..1620] *)) (PP (IN:[1621..1623] by) (NP-LGS (NN:[1624..1627] FSH)))))) (::[1627..1628] ;) (S (NP-SBJ (JJ:[1629..1636] maximal) (NN:[1637..1646] synthesis)) (VP (VBD:[1648..1656] occurred) (PP (RB:[1657..1661] only) (IN:[1662..1664] in) (NP (NP (DT:[1665..1668] the) (NN:[1669..1677] presence)) (PP (IN:[1678..1680] of) (NP (NN:[1681..1683] LH))))))) (.:[1683..1684] .))) ;sentence 12 Span:1685..1794 ;With both gonadotrophins at equivalent concentrations, LH generated a ;30-fold higher level of cAMP than FSH. ;[1695..1709]:substance:"gonadotrophins" ;[1741..1743]:substance:"LH" ;[1756..1770]:quantitative-value:"30-fold higher" ;[1780..1784]:substance:"cAMP" ;[1790..1793]:substance:"FSH" (SENT (S (PP (IN:[1685..1689] With) (NP (NP (DT:[1690..1694] both) (NNS:[1695..1709] gonadotrophins)) (PP (IN:[1710..1712] at) (NP (JJ:[1713..1723] equivalent) (NNS:[1725..1739] concentrations))))) (,:[1739..1740] ,) (NP-SBJ (NN:[1741..1743] LH)) (VP (VBD:[1744..1753] generated) (NP (NP (DT:[1754..1755] a) (ADJP (NML-EXT (CD:[1756..1758] 30) (HYPH:[1758..1759] -) (RB:[1759..1763] fold)) (JJR:[1764..1770] higher)) (NN:[1771..1776] level)) (PP (IN:[1777..1779] of) (NP (NN:[1780..1784] cAMP))) (PP (IN:[1785..1789] than) (NP (NN:[1790..1793] FSH))))) (.:[1793..1794] .))) ;sentence 13 Span:1796..1960 ;Furthermore, the differential effects of FSH and LH on P450 mRNA expression ;were reproduced by the presence of low and high concentrations of dbcAMP ;respectively. ;[1837..1840]:substance:"FSH" ;[1845..1847]:substance:"LH" ;[1851..1860]:substance:"P450 mRNA" ;[1939..1945]:substance:"dbcAMP" (SENT (S (ADVP (RB:[1796..1807] Furthermore)) (,:[1807..1808] ,) (NP-SBJ-2 (NP (DT:[1809..1812] the) (JJ:[1813..1825] differential) (NNS:[1826..1833] effects)) (PP (IN:[1834..1836] of) (NP (NN:[1837..1840] FSH) (CC:[1841..1844] and) (NN:[1845..1847] LH))) (PP (IN:[1848..1850] on) (NP (NN:[1851..1855] P450) (NN:[1856..1860] mRNA) (NN:[1861..1871] expression)))) (VP (VBD:[1872..1876] were) (VP (VBN:[1878..1888] reproduced) (NP-2 (-NONE-:[1888..1888] *)) (PP (IN:[1889..1891] by) (NP (NP (DT:[1892..1895] the) (NN:[1896..1904] presence)) (PP (IN:[1905..1907] of) (NP (NP (NP (JJ:[1908..1911] low) (NML-1 (-NONE-:[1911..1911] *P*))) (CC:[1912..1915] and) (NP (JJ:[1916..1920] high) (NML-1 (NNS:[1921..1935] concentrations)))) (PP (IN:[1936..1938] of) (NP (NN:[1939..1945] dbcAMP))))))) (ADVP (RB:[1947..1959] respectively)))) (.:[1959..1960] .))) ;sentence 14 Span:1961..2112 ;LH (and high levels of dbcAMP) increased P450arom mRNA expression in mature ;granulosa cells but inhibited its accumulation in granulosa lutein cells. ;[1961..1963]:substance:"LH" ;[1984..1990]:substance:"dbcAMP" ;[2002..2015]:substance:"P450arom mRNA" (SENT (S (NP-SBJ (NN:[1961..1963] LH) (PRN (-LRB-:[1964..1965] -LRB-) (FRAG (CC:[1965..1968] and) (NP (NP (JJ:[1969..1973] high) (NNS:[1974..1980] levels)) (PP (IN:[1981..1983] of) (NP (NN:[1984..1990] dbcAMP))))) (-RRB-:[1990..1991] -RRB-))) (VP (VP (VBD:[1992..2001] increased) (NP (NN:[2002..2010] P450arom) (NN:[2011..2015] mRNA) (NN:[2016..2026] expression)) (PP-LOC (IN:[2028..2030] in) (NP (JJ:[2031..2037] mature) (NN:[2038..2047] granulosa) (NNS:[2048..2053] cells)))) (CC:[2054..2057] but) (VP (VBD:[2058..2067] inhibited) (NP (PRP$:[2068..2071] its) (NN:[2072..2084] accumulation)) (PP-LOC (IN:[2085..2087] in) (NP (NN:[2088..2097] granulosa) (NN:[2098..2104] lutein) (NNS:[2106..2111] cells))))) (.:[2111..2112] .))) ;sentence 15 Span:2113..2244 ;In contrast, it stimulated P450scc mRNA expression and progesterone ;synthesis in both mature granulosa and granulosa lutein cells. ;[2140..2152]:substance:"P450scc mRNA" ;[2168..2180]:substance:"progesterone" (SENT (S (PP (IN:[2113..2115] In) (NP (NN:[2116..2124] contrast))) (,:[2124..2125] ,) (NP-SBJ (PRP:[2126..2128] it)) (VP (VBD:[2129..2139] stimulated) (NP (NP (NN:[2140..2147] P450scc) (NN:[2148..2152] mRNA) (NN:[2153..2163] expression)) (CC:[2164..2167] and) (NP (NN:[2168..2180] progesterone) (NN:[2182..2191] synthesis))) (PP-LOC (IN:[2192..2194] in) (NP (CC:[2195..2199] both) (NP (JJ:[2200..2206] mature) (NN:[2207..2216] granulosa)) (CC:[2217..2220] and) (NP (NN:[2221..2230] granulosa) (NN:[2231..2237] lutein) (NNS:[2238..2243] cells))))) (.:[2243..2244] .))) ;sentence 16 Span:2245..2500 ;Therefore, FSH/low cAMP levels stimulated P450arom gene expression and ;oestradiol production, while LH/high cAMP levels maximally induced P450scc ;gene expression and function, in a development-related manner consistent ;with steroid production in vivo. ;[2257..2260]:substance:"FSH" ;[2265..2269]:substance:"cAMP" ;[2317..2327]:substance:"oestradiol" ;[2347..2349]:substance:"LH" ;[2355..2359]:substance:"cAMP" ;[2472..2479]:substance:"steroid" (SENT (S (ADVP (RB:[2245..2254] Therefore)) (,:[2254..2255] ,) (S (NP-SBJ (NP (NN:[2257..2260] FSH)) (SYM:[2260..2261] /) (NP (JJ:[2261..2264] low) (NN:[2265..2269] cAMP) (NNS:[2270..2276] levels))) (VP (VBD:[2277..2287] stimulated) (NP (NP (NN:[2288..2296] P450arom) (NN:[2297..2301] gene) (NN:[2302..2312] expression)) (CC:[2313..2316] and) (NP (NN:[2317..2327] oestradiol) (NN:[2329..2339] production))) (,:[2339..2340] ,) (SBAR-ADV (IN:[2341..2346] while) (S (NP-SBJ (NP (NN:[2347..2349] LH)) (SYM:[2349..2350] /) (NP (JJ:[2350..2354] high) (NN:[2355..2359] cAMP) (NNS:[2360..2366] levels))) (ADVP (RB:[2367..2376] maximally)) (VP (VBD:[2377..2384] induced) (NP (NP (NML-1 (NN:[2385..2392] P450scc) (NN:[2393..2397] gene)) (NN:[2398..2408] expression)) (CC:[2410..2413] and) (NP (NML-1 (-NONE-:[2413..2413] *P*)) (NN:[2414..2422] function))) (,:[2422..2423] ,) (PP-MNR (IN:[2424..2426] in) (NP (NP (DT:[2427..2428] a) (ADJP (NN:[2429..2440] development) (HYPH:[2440..2441] -) (VBN:[2441..2448] related)) (NN:[2449..2455] manner)) (ADJP (JJ:[2456..2466] consistent) (PP (IN:[2467..2471] with) (NP (NP (JJ:[2472..2479] steroid) (NN:[2480..2490] production)) (ADJP (FW:[2492..2494] in) (FW:[2495..2499] vivo)))))))))))) (.:[2499..2500] .))) ;sentence 17 Span:2501..2690 ;These findings support the hypothesis that one set of genes (like P450arom) ;in human granulosa cells is regulated by FSH/low cAMP levels and another ;(like P450scc) by LH/high cAMP levels. ;[2619..2622]:substance:"FSH" ;[2627..2631]:substance:"cAMP" ;[2670..2672]:substance:"LH" ;[2678..2682]:substance:"cAMP" (SENT (S (NP-SBJ (DT:[2501..2506] These) (NNS:[2507..2515] findings)) (VP (VBP:[2516..2523] support) (NP (DT:[2524..2527] the) (NN:[2528..2538] hypothesis) (SBAR (IN:[2539..2543] that) (S (S (NP-SBJ-4=1 (NP (CD:[2544..2547] one) (NN:[2548..2551] set)) (PP (IN:[2552..2554] of) (NP (NNS:[2555..2560] genes))) (PRN (-LRB-:[2561..2562] -LRB-) (PP (IN:[2562..2566] like) (NP (NN:[2568..2576] P450arom))) (-RRB-:[2576..2577] -RRB-)) (PP-LOC (IN:[2578..2580] in) (NP (JJ:[2581..2586] human) (NN:[2587..2596] granulosa) (NNS:[2597..2602] cells)))) (VP=2 (VBZ:[2603..2605] is) (VP (VBN:[2606..2615] regulated) (NP-4 (-NONE-:[2615..2615] *)) (PP-MNR=3 (IN:[2616..2618] by) (NP (NP (NN:[2619..2622] FSH)) (SYM:[2622..2623] /) (NP (JJ:[2623..2626] low) (NN:[2627..2631] cAMP) (NNS:[2632..2638] levels))))))) (CC:[2639..2642] and) (S (NP-SBJ=1 (DT:[2644..2651] another) (PRN (-LRB-:[2652..2653] -LRB-) (PP (IN:[2653..2657] like) (NP (NN:[2658..2665] P450scc))) (-RRB-:[2665..2666] -RRB-))) (VP=2 (PP-MNR=3 (IN:[2667..2669] by) (NP (NP (NN:[2670..2672] LH)) (SYM:[2672..2673] /) (NP (JJ:[2673..2677] high) (NN:[2678..2682] cAMP) (NNS:[2683..2689] levels)))))))))) (.:[2689..2690] .))) ;section 18 Span:2694..2738 ;PMID: 8060487 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[2694..2698] PMID) (::[2698..2699] :) (CD:[2700..2707] 8060487) (NN:[2708..2709] -LSB-) (NNP:[2709..2715] PubMed) (::[2716..2717] -) (NN:[2718..2725] indexed) (IN:[2726..2729] for) (NNP:[2730..2737] MEDLINE) (-RRB-:[2737..2738] -RSB-)))