;PMID: 9374477 ;source_file_1916.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..42] = [t:0..42] ;1)sentence:[e:48..144] = [t:48..144] ;2)sentence:[e:145..195] = [t:145..195] ;3)section:[e:199..261] = [t:199..261] ;4)section:[e:265..427] = [t:265..427] ;5)sentence:[e:431..653] = [t:431..653] ;6)sentence:[e:654..742] = [t:654..742] ;7)sentence:[e:743..853] = [t:743..853] ;8)sentence:[e:854..939] = [t:854..939] ;9)sentence:[e:940..1105] = [t:940..1105] ;10)sentence:[e:1106..1239] = [t:1106..1239] ;11)sentence:[e:1240..1447] = [t:1240..1447] ;12)sentence:[e:1448..1669] = [t:1448..1669] ;13)section:[e:1673..1717] = [t:1673..1717] ;section 0 Span:0..42 ;J Biol Chem. 1997 Nov 28;272(48):30025-31. (SEC (FRAG (NNP:[0..1] J) (NNP:[2..6] Biol) (NNP:[7..11] Chem) (.:[11..12] .) (CD:[13..17] 1997) (NNP:[18..21] Nov) (CD:[22..27] 28;27) (CD:[27..28] 2) (NN:[28..29] -LRB-) (CD:[29..31] 48) (-RRB-:[31..32] -RRB-) (::[32..33] :) (CD:[33..37] 3002) (CD:[37..41] 5-31) (.:[41..42] .))) ;sentence 1 Span:48..144 ;Inhibition of the transcription of CYP1A1 gene by the upstream stimulatory ;factor 1 in rabbits. ;[124..132]:substance:"factor 1" (SENT (NP-HLN (NP (NN:[48..58] Inhibition)) (PP (IN:[59..61] of) (NP (NP (DT:[62..65] the) (NN:[66..79] transcription)) (PP (IN:[80..82] of) (NP (NN:[83..89] CYP1A1) (NN:[90..94] gene))))) (PP (IN:[95..97] by) (NP (DT:[98..101] the) (JJ:[102..110] upstream) (JJ:[111..122] stimulatory) (NN:[124..130] factor) (CD:[131..132] 1))) (PP-LOC (IN:[133..135] in) (NP (NNS:[136..143] rabbits))) (.:[143..144] .))) ;sentence 2 Span:145..195 ;Competitive binding of USF1 with AhR.Arnt complex. ;[168..172]:substance:"USF1" ;[178..194]:substance:"AhR.Arnt complex" (SENT (NP-HLN (NP (JJ:[145..156] Competitive) (NN:[157..164] binding)) (PP (IN:[165..167] of) (NP (NN:[168..172] USF1))) (PP (IN:[173..177] with) (NP (NN:[178..186] AhR.Arnt) (NN:[187..194] complex))) (.:[194..195] .))) ;section 3 Span:199..261 ;Takahashi Y, Nakayama K, Itoh S, Fujii-Kuriyama Y, Kamataki T. (SEC (FRAG (NNP:[199..208] Takahashi) (NNP:[209..210] Y) (,:[210..211] ,) (NNP:[212..220] Nakayama) (NNP:[221..223] K,) (NNP:[224..228] Itoh) (NNP:[229..230] S) (,:[230..231] ,) (NNP:[232..238] Fujii-) (NNP:[238..246] Kuriyama) (NNP:[247..248] Y) (,:[248..249] ,) (NNP:[250..258] Kamataki) (NNP:[259..261] T.))) ;section 4 Span:265..427 ;Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido ;University, N12W6, Kita-ku, Sapporo, Hokkaido 060, Japan. ;kamataki@pharm.hokudai.ac.jp (SEC (FRAG (NNP:[265..273] Division) (IN:[274..276] of) (NNP:[277..281] Drug) (NNP:[282..292] Metabolism) (,:[292..293] ,) (NNP:[294..301] Faculty) (IN:[302..304] of) (NNP:[305..319] Pharmaceutical) (NNPS:[320..328] Sciences) (,:[328..329] ,) (NNP:[330..338] Hokkaido) (NNP:[340..350] University) (,:[350..351] ,) (NNP:[352..357] N12W6) (,:[357..358] ,) (NN:[359..366] Kita-ku) (,:[366..367] ,) (NNP:[368..375] Sapporo) (,:[375..376] ,) (NNP:[377..385] Hokkaido) (CD:[386..389] 060) (,:[389..390] ,) (NNP:[391..396] Japan) (.:[396..397] .) (``:[399..413] kamataki@pharm) (NN:[413..427] .hokudai.ac.jp))) ;sentence 5 Span:431..653 ;A xenobiotic-responsive element (XRE)-binding factor(s) other than the ;AhR.Arnt complex was found to inhibit the transcription of CYP1A1 gene in ;the liver from adult rabbits, known to be nonresponsive to CYP1A1 inducers. ;[433..486]:substance:"xenobiotic-responsive element (XRE)-binding factor(s)" ;[502..519]:substance:"AhR.Arnt complex" ;[637..643]:cyp450:"CYP1A1" ;[644..652]:substance:"inducers" (SENT (S (NP-SBJ-1 (NP (DT:[431..432] A) (ADJP (NML (NML (NML (JJ:[433..443] xenobiotic) (HYPH:[443..444] -) (JJ:[444..454] responsive)) (NN:[455..462] element)) (NML (-LRB-:[463..464] -LRB-) (NN:[464..467] XRE) (-RRB-:[467..468] -RRB-))) (HYPH:[468..469] -) (VBG:[469..476] binding)) (NN:[477..486] factor-LRB-s-RRB-)) (ADJP (JJ:[487..492] other) (PP (IN:[493..497] than) (NP (DT:[498..501] the) (NN:[502..510] AhR.Arnt) (NN:[512..519] complex))))) (VP (VBD:[520..523] was) (VP (VBN:[524..529] found) (S (NP-SBJ-1 (-NONE-:[529..529] *)) (VP (TO:[530..532] to) (VP (VB:[533..540] inhibit) (NP (NP (DT:[541..544] the) (NN:[545..558] transcription)) (PP (IN:[559..561] of) (NP (NP (NP (NN:[562..568] CYP1A1) (NN:[569..573] gene)) (PP-LOC (IN:[574..576] in) (NP (NP (DT:[577..580] the) (NN:[581..586] liver)) (PP (IN:[587..591] from) (NP (JJ:[593..598] adult) (NNS:[599..606] rabbits)))))) (,:[606..607] ,) (VP (VBN:[608..613] known) (S (NP-SBJ (-NONE-:[613..613] *)) (VP (TO:[614..616] to) (VP (VB:[617..619] be) (ADJP-PRD (JJ:[620..633] nonresponsive) (PP (TO:[634..636] to) (NP (NN:[637..643] CYP1A1) (NNS:[644..652] inducers)))))))))))))))) (.:[652..653] .))) ;sentence 6 Span:654..742 ;The constitutive factor(s) in liver nuclear extracts bound to the core ;sequence of XRE. ;[738..741]:substance:"XRE" (SENT (S (NP-SBJ (NP (DT:[654..657] The) (JJ:[658..670] constitutive) (NN:[672..681] factor-LRB-s-RRB-)) (PP-LOC (IN:[682..684] in) (NP (NN:[685..690] liver) (JJ:[691..698] nuclear) (NNS:[699..707] extracts)))) (VP (VBD:[708..713] bound) (PP (TO:[714..716] to) (NP (NP (DT:[717..720] the) (NN:[721..725] core) (NN:[726..734] sequence)) (PP (IN:[735..737] of) (NP (NN:[738..741] XRE)))))) (.:[741..742] .))) ;sentence 7 Span:743..853 ;The binding was eliminated by the presence of an excess amount of the ;AhR.Arnt complex synthesized in vitro. ;[814..831]:substance:"AhR.Arnt complex" (SENT (S (NP-SBJ-1 (DT:[743..746] The) (NN:[748..755] binding)) (VP (VBD:[756..759] was) (VP (VBN:[760..770] eliminated) (NP-1 (-NONE-:[770..770] *)) (PP-MNR (IN:[771..773] by) (NP (NP (DT:[774..777] the) (NN:[778..786] presence)) (PP (IN:[787..789] of) (NP (NP (DT:[790..792] an) (JJ:[793..799] excess) (NN:[800..806] amount)) (PP (IN:[807..809] of) (NP (NP (DT:[810..813] the) (NN:[814..822] AhR.Arnt) (NN:[824..831] complex)) (VP (VBN:[832..843] synthesized) (NP (-NONE-:[843..843] *)) (ADVP (FW:[844..846] in) (FW:[847..852] vitro))))))))))) (.:[852..853] .))) ;sentence 8 Span:854..939 ;To identify the constitutive factor(s), a sequence similar to rabbit XRE was ;sought. ;[924..927]:substance:"XRE" (SENT (S (S-PRP (NP-SBJ (-NONE-:[854..854] *)) (VP (TO:[854..856] To) (VP (VB:[857..865] identify) (NP (DT:[866..869] the) (JJ:[870..882] constitutive) (NN:[883..892] factor-LRB-s-RRB-))))) (,:[892..893] ,) (NP-SBJ-1 (NP (DT:[894..895] a) (NN:[896..904] sequence)) (ADJP (JJ:[906..913] similar) (PP (TO:[914..916] to) (NP (NN:[917..923] rabbit) (NN:[924..927] XRE))))) (VP (VBD:[928..931] was) (VP (VBN:[932..938] sought) (NP-1 (-NONE-:[938..938] *)))) (.:[938..939] .))) ;sentence 9 Span:940..1105 ;It was found that the sequence of rabbit XRE overlapped with that of the ;upstream stimulatory factor 1 (USF1)-binding site in the mouse ;metallothionein I promoter. ;[981..984]:substance:"XRE" ;[1014..1043]:substance:"upstream stimulatory factor 1" ;[1045..1049]:substance:"USF1" ;[1078..1095]:substance:"metallothionein I" ;[1096..1104]:substance:"promoter" (SENT (S (NP-SBJ-1 (NP (PRP:[940..942] It)) (SBAR-2 (-NONE-:[942..942] *ICH*))) (VP (VBD:[943..946] was) (VP (VBN:[947..952] found) (NP-1 (-NONE-:[952..952] *)) (SBAR-2 (IN:[953..957] that) (S (NP-SBJ (NP (DT:[958..961] the) (NN:[962..970] sequence)) (PP (IN:[971..973] of) (NP (NN:[974..980] rabbit) (NN:[981..984] XRE)))) (VP (VBD:[986..996] overlapped) (PP (IN:[997..1001] with) (NP (NP (DT:[1002..1006] that)) (PP (IN:[1007..1009] of) (NP (DT:[1010..1013] the) (ADJP (NML (NML (JJ:[1014..1022] upstream) (JJ:[1023..1034] stimulatory) (NN:[1035..1041] factor) (CD:[1042..1043] 1)) (NML (-LRB-:[1044..1045] -LRB-) (NN:[1045..1049] USF1) (-RRB-:[1049..1050] -RRB-))) (HYPH:[1050..1051] -) (VBG:[1051..1058] binding)) (NN:[1059..1063] site))))) (PP-LOC (IN:[1064..1066] in) (NP (DT:[1068..1071] the) (NN:[1072..1077] mouse) (NML (NN:[1078..1093] metallothionein) (CD:[1094..1095] I)) (NN:[1096..1104] promoter)))))))) (.:[1104..1105] .))) ;sentence 10 Span:1106..1239 ;In fact, a super shift assay using a specific antibody against human USF1 ;indicated that USF1 was capable of binding to rabbit XRE. ;[1153..1161]:substance:"antibody" ;[1176..1180]:substance:"USF1" ;[1196..1200]:substance:"USF1" ;[1235..1238]:substance:"XRE" (SENT (S (PP (IN:[1106..1108] In) (NP (NN:[1109..1113] fact))) (,:[1113..1114] ,) (NP-SBJ (NP (DT:[1115..1116] a) (NML (JJ:[1117..1122] super) (NN:[1123..1128] shift)) (NN:[1129..1134] assay)) (VP (VBG:[1135..1140] using) (NP (NP (DT:[1141..1142] a) (JJ:[1144..1152] specific) (NN:[1153..1161] antibody)) (PP (IN:[1162..1169] against) (NP (JJ:[1170..1175] human) (NN:[1176..1180] USF1)))))) (VP (VBD:[1181..1190] indicated) (SBAR (IN:[1191..1195] that) (S (NP-SBJ (NN:[1196..1200] USF1)) (VP (VBD:[1201..1204] was) (ADJP-PRD (JJ:[1205..1212] capable) (PP (IN:[1213..1215] of) (S-NOM (NP-SBJ (-NONE-:[1215..1215] *)) (VP (VBG:[1216..1223] binding) (PP (TO:[1225..1227] to) (NP (NN:[1228..1234] rabbit) (NN:[1235..1238] XRE))))))))))) (.:[1238..1239] .))) ;sentence 11 Span:1240..1447 ;Additionally, the AhR.Arnt-mediated activation of XRE-TK/Luc reporter gene ;in RK13 cells was blocked by the transfection with a USF1 expression vector ;with the amounts of the expression vector transfected. ;[1258..1266]:substance:"AhR.Arnt" ;[1369..1373]:substance:"USF1" (SENT (S (ADVP (RB:[1240..1252] Additionally)) (,:[1252..1253] ,) (NP-SBJ-1 (NP (DT:[1254..1257] the) (ADJP (NN:[1258..1266] AhR.Arnt) (HYPH:[1266..1267] -) (VBN:[1267..1275] mediated)) (NN:[1276..1286] activation)) (PP (IN:[1287..1289] of) (NP (NP (NML (NN:[1290..1296] XRE-TK) (SYM:[1296..1297] /) (NN:[1297..1300] Luc)) (NN:[1302..1310] reporter) (NN:[1311..1315] gene)) (PP (IN:[1316..1318] in) (NP (NN:[1319..1323] RK13) (NNS:[1324..1329] cells)))))) (VP (VBD:[1330..1333] was) (VP (VBN:[1334..1341] blocked) (NP-1 (-NONE-:[1341..1341] *)) (PP (IN:[1342..1344] by) (NP-LGS (NP (DT:[1345..1348] the) (NN:[1349..1361] transfection)) (PP (IN:[1362..1366] with) (NP (DT:[1367..1368] a) (NN:[1369..1373] USF1) (NN:[1375..1385] expression) (NN:[1386..1392] vector))))) (PP (IN:[1393..1397] with) (NP (NP (DT:[1398..1401] the) (NNS:[1402..1409] amounts)) (PP (IN:[1410..1412] of) (NP (NP (DT:[1413..1416] the) (NN:[1417..1427] expression) (NN:[1428..1434] vector)) (VP (VBN:[1435..1446] transfected) (NP (-NONE-:[1446..1446] *))))))))) (.:[1446..1447] .))) ;sentence 12 Span:1448..1669 ;These results indicate that the XRE of the rabbit CYP1A1 gene is recognized ;by the basic helix-loop-helix proteins to regulate the expression of CYP1A1 ;in both an agonistic (AhR.Arnt) and an antagonistic (USF1) manner. ;[1481..1484]:substance:"XRE" ;[1595..1601]:cyp450:"CYP1A1" ;[1625..1633]:substance:"AhR.Arnt" ;[1656..1660]:substance:"USF1" (SENT (S (NP-SBJ (DT:[1448..1453] These) (NNS:[1455..1462] results)) (VP (VBP:[1463..1471] indicate) (SBAR (IN:[1472..1476] that) (S (NP-SBJ-2 (NP (DT:[1477..1480] the) (NN:[1481..1484] XRE)) (PP (IN:[1485..1487] of) (NP (DT:[1488..1491] the) (NN:[1492..1498] rabbit) (NN:[1499..1505] CYP1A1) (NN:[1506..1510] gene)))) (VP (VBZ:[1511..1513] is) (VP (VBN:[1514..1524] recognized) (NP-2 (-NONE-:[1524..1524] *)) (PP (IN:[1525..1527] by) (NP-LGS (DT:[1528..1531] the) (JJ:[1533..1538] basic) (NML (NN:[1539..1544] helix) (HYPH:[1544..1545] -) (NN:[1545..1549] loop) (HYPH:[1549..1550] -) (NN:[1550..1555] helix)) (NNS:[1556..1564] proteins))) (S-ADV (NP-SBJ (-NONE-:[1564..1564] *)) (VP (TO:[1565..1567] to) (VP (VB:[1568..1576] regulate) (NP (NP (DT:[1577..1580] the) (NN:[1581..1591] expression)) (PP (IN:[1592..1594] of) (NP (NN:[1595..1601] CYP1A1)))) (PP (IN:[1602..1604] in) (NP (CC:[1605..1609] both) (NP (NP (DT:[1610..1612] an) (ADJP (JJ:[1614..1623] agonistic) (PRN (-LRB-:[1624..1625] -LRB-) (NP (NN:[1625..1633] AhR.Arnt)) (-RRB-:[1633..1634] -RRB-))) (NML-1 (-NONE-:[1634..1634] *P*))) (CC:[1635..1638] and) (NP (DT:[1639..1641] an) (ADJP (JJ:[1642..1654] antagonistic) (PRN (-LRB-:[1655..1656] -LRB-) (NP (NN:[1656..1660] USF1)) (-RRB-:[1660..1661] -RRB-))) (NML-1 (NN:[1662..1668] manner)))))))))))))) (.:[1668..1669] .))) ;section 13 Span:1673..1717 ;PMID: 9374477 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1673..1677] PMID) (::[1677..1678] :) (CD:[1679..1686] 9374477) (NN:[1687..1688] -LSB-) (NNP:[1688..1694] PubMed) (::[1695..1696] -) (NN:[1697..1704] indexed) (IN:[1705..1708] for) (NNP:[1709..1717] MEDLINE-RSB-)))