;PMID: 3345196 ;source_file_1799.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..43] = [t:0..43] ;1)sentence:[e:49..145] = [t:49..145] ;2)section:[e:149..164] = [t:149..164] ;3)section:[e:168..253] = [t:168..253] ;4)sentence:[e:257..402] = [t:257..402] ;5)sentence:[e:403..536] = [t:403..536] ;6)sentence:[e:537..819] = [t:537..819] ;7)sentence:[e:820..1147] = [t:820..1147] ;8)sentence:[e:1148..1348] = [t:1148..1348] ;9)sentence:[e:1349..1617] = [t:1349..1617] ;10)sentence:[e:1618..1721] = [t:1618..1721] ;11)section:[e:1725..1769] = [t:1725..1769] ;section 0 Span:0..43 ;Biochem Pharmacol. 1988 Mar 1;37(5):781-91. (SEC (FRAG (NNP:[0..7] Biochem) (NNP:[8..17] Pharmacol) (.:[17..18] .) (CD:[19..23] 1988) (NNP:[24..27] Mar) (CD:[28..32] 1;37) (-LRB-:[32..33] -LRB-) (CD:[33..34] 5) (-RRB-:[34..35] -RRB-) (CD:[35..39] :781) (::[39..40] -) (CD:[40..42] 91) (.:[42..43] .))) ;sentence 1 Span:49..145 ;Stimulation of the conjugation of lipid dienes in hepatic microsomes by ;3,3'-dichlorobenzidine. ;[83..95]:substance:"lipid dienes" ;[122..144]:substance:"3,3'-dichlorobenzidine" (SENT (NP-HLN (NP (NN:[49..60] Stimulation)) (PP (IN:[61..63] of) (NP (NP (DT:[64..67] the) (NN:[68..79] conjugation)) (PP (IN:[80..82] of) (NP (NN:[83..88] lipid) (NNS:[89..95] dienes))))) (PP-LOC (IN:[96..98] in) (NP (JJ:[99..106] hepatic) (NNS:[107..117] microsomes))) (PP (IN:[118..120] by) (NP (NN:[122..144] 3,3'-dichlorobenzidine))) (.:[144..145] .))) ;section 2 Span:149..164 ;Iba MM, Lang B. (SEC (FRAG (NNP:[149..152] Iba) (NNP:[153..155] MM) (,:[155..156] ,) (NNP:[157..161] Lang) (NNP:[162..164] B.))) ;section 3 Span:168..253 ;Department of Pharmacology and Toxicology, Rutgers University, Piscataway, NJ ; 08854. (SEC (FRAG (NNP:[168..178] Department) (IN:[179..181] of) (NNP:[182..194] Pharmacology) (CC:[195..198] and) (NNP:[199..209] Toxicology) (,:[209..210] ,) (NNP:[211..218] Rutgers) (NNP:[219..229] University) (,:[229..230] ,) (NNP:[231..241] Piscataway) (,:[241..242] ,) (NNP:[243..245] NJ) (CD:[247..252] 08854) (.:[252..253] .))) ;sentence 4 Span:257..402 ;Pretreatment of male rats with 3,3'-dichlorobenzidine (DCB) resulted in the ;accumulation of conjugated dienes in lipids from hepatic microsomes. ;[288..310]:substance:"3,3'-dichlorobenzidine" ;[312..315]:substance:"DCB" ;[361..367]:substance:"dienes" ;[371..377]:substance:"lipids" (SENT (S (NP-SBJ (NP (NN:[257..269] Pretreatment)) (PP (IN:[270..272] of) (NP (JJ:[273..277] male) (NNS:[278..282] rats))) (PP (IN:[283..287] with) (NP (NP (NN:[288..310] 3,3'-dichlorobenzidine)) (NP (-LRB-:[311..312] -LRB-) (NN:[312..315] DCB) (-RRB-:[315..316] -RRB-))))) (VP (VBD:[317..325] resulted) (PP-CLR (IN:[326..328] in) (NP (NP (DT:[329..332] the) (NN:[334..346] accumulation)) (PP (IN:[347..349] of) (NP (VBN:[350..360] conjugated) (NNS:[361..367] dienes))))) (PP-LOC (IN:[368..370] in) (NP (NP (NNS:[371..377] lipids)) (PP (IN:[378..382] from) (NP (JJ:[383..390] hepatic) (NNS:[391..401] microsomes)))))) (.:[401..402] .))) ;sentence 5 Span:403..536 ;In vitro, these microsomes had 2-fold the NADPH-dependent malondialdehyde ;(MDA)-forming capacity of microsomes from untreated rats. ;[435..441]:quantitative-value:"2-fold" ;[446..451]:substance:"NADPH" ;[462..477]:substance:"malondialdehyde" ;[479..482]:substance:"MDA" (SENT (S (ADVP (FW:[403..405] In) (FW:[406..411] vitro)) (,:[411..412] ,) (NP-SBJ (DT:[414..419] these) (NNS:[420..430] microsomes)) (VP (VBD:[431..434] had) (NP (NP (ADVP (CD:[435..436] 2) (HYPH:[436..437] -) (RB:[437..441] fold)) (DT:[442..445] the) (ADJP (NN:[446..451] NADPH) (HYPH:[451..452] -) (JJ:[452..461] dependent)) (ADJP (NML (NML (NN:[462..477] malondialdehyde)) (NML (-LRB-:[478..479] -LRB-) (NN:[479..482] MDA) (-RRB-:[482..483] -RRB-))) (HYPH:[483..484] -) (VBG:[484..491] forming)) (NN:[493..501] capacity)) (PP (IN:[502..504] of) (NP (NP (NNS:[505..515] microsomes)) (PP (IN:[516..520] from) (NP (JJ:[521..530] untreated) (NNS:[531..535] rats))))))) (.:[535..536] .))) ;sentence 6 Span:537..819 ;To determine the mechanisms of the DCB-induced accumulation of diene ;conjugation, the effects of added DCB on NADPH- or iron + ascorbic acid- ;(Fe2+-ascorbate-) dependent diene conjugation, oxygen uptake and MDA ;formation were examined in microsomes from untreated rats in vitro. ;[573..576]:substance:"DCB" ;[601..606]:substance:"diene" ;[641..644]:substance:"DCB" ;[649..654]:substance:"NADPH" ;[659..663]:substance:"iron" ;[666..679]:substance:"ascorbic acid" ;[682..685]:substance:"Fe2" ;[687..696]:substance:"ascorbate" ;[709..714]:substance:"diene" ;[729..735]:substance:"oxygen" ;[747..750]:substance:"MDA" (SENT (S (S-PRP (NP-SBJ (-NONE-:[537..537] *)) (VP (TO:[537..539] To) (VP (VB:[540..549] determine) (NP (NP (DT:[550..553] the) (NNS:[554..564] mechanisms)) (PP (IN:[565..567] of) (NP (NP (DT:[568..571] the) (ADJP (NN:[573..576] DCB) (HYPH:[576..577] -) (VBN:[577..584] induced)) (NN:[585..597] accumulation)) (PP (IN:[598..600] of) (NP (NN:[601..606] diene) (NN:[607..618] conjugation))))))))) (,:[618..619] ,) (NP-SBJ-3 (NP (DT:[620..623] the) (NNS:[624..631] effects)) (PP (IN:[632..634] of) (NP (VBN:[635..640] added) (NN:[641..644] DCB))) (PP (IN:[645..647] on) (NP (NP (NP (ADJP (NN:[649..654] NADPH) (HYPH:[654..655] -) (ADJP-2 (-NONE-:[655..655] *P*))) (NML-1 (-NONE-:[655..655] *P*))) (CC:[656..658] or) (NP (ADJP (NML (NML (NML (NN:[659..663] iron)) (SYM:[664..665] +) (NML (JJ:[666..674] ascorbic) (NN:[675..679] acid))) (HYPH:[679..680] -) (NML (-LRB-:[681..682] -LRB-) (NN:[682..685] Fe2) (SYM:[685..686] +) (HYPH:[686..687] -) (NN:[687..696] ascorbate) (HYPH:[696..697] -) (-RRB-:[697..698] -RRB-))) (ADJP-2 (JJ:[699..708] dependent))) (NML-1 (NN:[709..714] diene) (NN:[715..726] conjugation)))) (,:[726..727] ,) (NP (NN:[729..735] oxygen) (NN:[736..742] uptake)) (CC:[743..746] and) (NP (NN:[747..750] MDA) (NN:[751..760] formation))))) (VP (VBD:[761..765] were) (VP (VBN:[766..774] examined) (NP-3 (-NONE-:[774..774] *)) (PP-LOC (IN:[775..777] in) (NP (NP (NNS:[778..788] microsomes)) (PP (IN:[789..793] from) (NP (JJ:[794..803] untreated) (NNS:[804..808] rats))))) (ADVP (FW:[810..812] in) (FW:[813..818] vitro)))) (.:[818..819] .))) ;sentence 7 Span:820..1147 ;In the presence of NADPH, added DCB stimulated diene conjugation in ;microsomal lipids as did in vivo DCB pretreatment but inhibited the uptake of ; oxygen and the formation of MDA. When Fe2+-ascorbate was substituted for ;NADPH, the formation of diene conjugation, oxygen uptake, and MDA formation ;were inhibited by added DCB. ;[839..844]:substance:"NADPH" ;[852..855]:substance:"DCB" ;[867..872]:substance:"diene" ;[900..906]:substance:"lipids" ;[922..925]:substance:"DCB" ;[968..974]:substance:"oxygen" ;[996..999]:substance:"MDA" ;[1006..1010]:substance:"Fe2+" ;[1011..1020]:substance:"ascorbate" ;[1041..1046]:substance:"NADPH" ;[1066..1071]:substance:"diene" ;[1085..1091]:substance:"oxygen" ;[1104..1107]:substance:"MDA" ;[1143..1146]:substance:"DCB" (SENT (S (PP (IN:[820..822] In) (NP (NP (DT:[823..826] the) (NN:[827..835] presence)) (PP (IN:[836..838] of) (NP (NN:[839..844] NADPH))))) (,:[844..845] ,) (NP-SBJ (VBN:[846..851] added) (NN:[852..855] DCB)) (VP (VP (VBD:[856..866] stimulated) (NP (NN:[867..872] diene) (NN:[873..884] conjugation)) (PP-LOC (IN:[885..887] in) (NP (JJ:[889..899] microsomal) (NNS:[900..906] lipids))) (SBAR-ADV (IN:[907..909] as) (SINV (VBD:[910..913] did) (NP-SBJ (ADJP (FW:[914..916] in) (FW:[917..921] vivo)) (NN:[922..925] DCB) (NN:[926..938] pretreatment)) (VP (-NONE-:[938..938] *?*))))) (CC:[939..942] but) (VP (VBD:[943..952] inhibited) (NP (NP (NP (DT:[953..956] the) (NN:[957..963] uptake)) (PP (IN:[964..966] of) (NP (NN:[968..974] oxygen)))) (CC:[975..978] and) (NP (NP (DT:[979..982] the) (NN:[983..992] formation)) (PP (IN:[993..995] of) (NP (NN:[996..999] MDA))))))) (.:[999..1000] .)) (S (SBAR-TMP (WHADVP-2 (WRB:[1001..1005] When)) (S (NP-SBJ-1 (NML (NN:[1006..1009] Fe2) (SYM:[1009..1010] +)) (HYPH:[1010..1011] -) (NN:[1011..1020] ascorbate)) (VP (VBD:[1021..1024] was) (VP (VBN:[1025..1036] substituted) (NP-1 (-NONE-:[1036..1036] *)) (PP (IN:[1037..1040] for) (NP (NN:[1041..1046] NADPH))) (ADVP-2 (-NONE-:[1046..1046] *T*)))))) (,:[1046..1047] ,) (NP-SBJ-4 (NP (NP (DT:[1049..1052] the) (NN:[1053..1062] formation)) (PP (IN:[1063..1065] of) (NP (NN:[1066..1071] diene) (NN:[1072..1083] conjugation)))) (,:[1083..1084] ,) (NP (NN:[1085..1091] oxygen) (NN:[1092..1098] uptake)) (,:[1098..1099] ,) (CC:[1100..1103] and) (NP (NN:[1104..1107] MDA) (NN:[1108..1117] formation))) (VP (VBD:[1118..1122] were) (VP (VBN:[1124..1133] inhibited) (NP-4 (-NONE-:[1133..1133] *)) (PP (IN:[1134..1136] by) (NP-LGS (VBN:[1137..1142] added) (NN:[1143..1146] DCB))))) (.:[1146..1147] .))) ;ERROR_Sentence has multiple Children (Sentence Error)[820..1147]::S:S: ;sentence 8 Span:1148..1348 ;The DCB-induced stimulation of diene conjugation, in addition to being ;strictly NADPH dependent, was carbon monoxide sensitive and was concomitant ;with the binding of added DCB to microsomal lipids. ;[1152..1155]:substance:"DCB" ;[1179..1184]:substance:"diene" ;[1229..1234]:substance:"NADPH" ;[1250..1265]:substance:"carbon monoxide" ;[1323..1326]:substance:"DCB" ;[1341..1347]:substance:"lipids" (SENT (S (NP-SBJ (NP (NP (DT:[1148..1151] The) (ADJP (NN:[1152..1155] DCB) (HYPH:[1155..1156] -) (VBN:[1156..1163] induced)) (NN:[1164..1175] stimulation)) (PP (IN:[1176..1178] of) (NP (NN:[1179..1184] diene) (NN:[1185..1196] conjugation)))) (,:[1196..1197] ,) (PP (IN:[1198..1200] in) (NP (NP (NN:[1202..1210] addition)) (PP (TO:[1211..1213] to) (S-NOM (NP-SBJ (-NONE-:[1213..1213] *)) (VP (VBG:[1214..1219] being) (ADJP-PRD (RB:[1220..1228] strictly) (NN:[1229..1234] NADPH) (JJ:[1235..1244] dependent)))))))) (,:[1244..1245] ,) (VP (VP (VBD:[1246..1249] was) (ADJP-PRD (NML (NN:[1250..1256] carbon) (NN:[1257..1265] monoxide)) (JJ:[1266..1275] sensitive))) (CC:[1276..1279] and) (VP (VBD:[1281..1284] was) (ADJP-PRD (JJ:[1285..1296] concomitant) (PP (IN:[1297..1301] with) (NP (NP (DT:[1302..1305] the) (NN:[1306..1313] binding)) (PP (IN:[1314..1316] of) (NP (VBN:[1317..1322] added) (NN:[1323..1326] DCB))) (PP (TO:[1327..1329] to) (NP (JJ:[1330..1340] microsomal) (NNS:[1341..1347] lipids)))))))) (.:[1347..1348] .))) ;sentence 9 Span:1349..1617 ;It is postulated that a metabolite of DCB generated by cytochrome P-450 ;reacts with membrane lipids both in vivo and in vitro in a manner analogous ;to the initiation of lipid peroxidation but at the same time prevents the ;autocatalytic decomposition of the lipids. ;[1374..1384]:substance:"metabolite" ;[1388..1391]:substance:"DCB" ;[1405..1421]:cyp450:"cytochrome P-450" ;[1444..1450]:substance:"lipids" ;[1521..1526]:substance:"lipid" ;[1610..1616]:substance:"lipids" (SENT (S (NP-SBJ-1 (NP (PRP:[1349..1351] It)) (SBAR-2 (-NONE-:[1351..1351] *EXP*))) (VP (VBZ:[1352..1354] is) (VP (VBN:[1356..1366] postulated) (NP-1 (-NONE-:[1366..1366] *)) (SBAR-2 (IN:[1367..1371] that) (S (NP-SBJ (NP (NP (DT:[1372..1373] a) (NN:[1374..1384] metabolite)) (PP (IN:[1385..1387] of) (NP (NN:[1388..1391] DCB)))) (VP (VBN:[1392..1401] generated) (NP (-NONE-:[1401..1401] *)) (PP (IN:[1402..1404] by) (NP-LGS (NN:[1405..1415] cytochrome) (NN:[1416..1421] P-450))))) (VP (VP (VBZ:[1422..1428] reacts) (PP-CLR (IN:[1429..1433] with) (NP (NN:[1435..1443] membrane) (NNS:[1444..1450] lipids))) (ADVP (CC:[1451..1455] both) (ADVP (FW:[1456..1458] in) (FW:[1459..1463] vivo)) (CC:[1464..1467] and) (ADVP (FW:[1468..1470] in) (FW:[1471..1476] vitro))) (PP (IN:[1477..1479] in) (NP (NP (DT:[1480..1481] a) (NN:[1482..1488] manner)) (ADJP (JJ:[1489..1498] analogous) (PP (TO:[1499..1501] to) (NP (NP (DT:[1502..1505] the) (NN:[1507..1517] initiation)) (PP (IN:[1518..1520] of) (NP (NN:[1521..1526] lipid) (NN:[1527..1539] peroxidation))))))))) (CC:[1540..1543] but) (VP (PP-TMP (IN:[1544..1546] at) (NP (DT:[1547..1550] the) (JJ:[1551..1555] same) (NN:[1556..1560] time))) (VBZ:[1561..1569] prevents) (NP (NP (DT:[1570..1573] the) (JJ:[1574..1587] autocatalytic) (NN:[1589..1602] decomposition)) (PP (IN:[1603..1605] of) (NP (DT:[1606..1609] the) (NNS:[1610..1616] lipids)))))))))) (.:[1616..1617] .))) ;sentence 10 Span:1618..1721 ;The DCB-induced diene conjugation is interpreted as predisposing to ;deleterious changes in microsomes. ;[1622..1625]:substance:"DCB" ;[1634..1639]:substance:"diene" (SENT (S (NP-SBJ-1 (DT:[1618..1621] The) (ADJP (NN:[1622..1625] DCB) (HYPH:[1625..1626] -) (VBN:[1626..1633] induced)) (NN:[1634..1639] diene) (NN:[1640..1651] conjugation)) (VP (VBZ:[1652..1654] is) (VP (VBN:[1655..1666] interpreted) (NP-1 (-NONE-:[1666..1666] *)) (PP-CLR (IN:[1667..1669] as) (S-NOM (NP-SBJ (-NONE-:[1669..1669] *)) (VP (VBG:[1671..1683] predisposing) (PP (TO:[1684..1686] to) (NP (NP (JJ:[1687..1698] deleterious) (NNS:[1699..1706] changes)) (PP (IN:[1707..1709] in) (NP (NNS:[1710..1720] microsomes)))))))))) (.:[1720..1721] .))) ;section 11 Span:1725..1769 ;PMID: 3345196 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1725..1729] PMID) (::[1729..1730] :) (CD:[1731..1738] 3345196) (NN:[1739..1740] -LSB-) (NNP:[1740..1746] PubMed) (::[1747..1748] -) (NN:[1749..1756] indexed) (IN:[1757..1760] for) (NNP:[1761..1769] MEDLINE-RSB-)))