;PMID: 8182306 ;source_file_1791.mrg ;Release 0.9 of PennBioIE ;Funded by NSF ITR EIA-0205448 ;0)section:[e:0..58] = [t:0..58] ;1)sentence:[e:64..120] = [t:64..120] ;2)section:[e:124..145] = [t:124..145] ;3)section:[e:149..217] = [t:149..217] ;4)section:[e:221..304] = [t:221..304] ;5)section:[e:null] = [t:306..532] ;6)sentence:[e:308..532] = [t:null] ;7)sentence:[e:533..613] = [t:533..613] ;8)sentence:[e:614..737] = [t:614..737] ;9)sentence:[e:738..899] = [t:738..899] ;10)sentence:[e:900..1096] = [t:900..1096] ;11)sentence:[e:1098..1267] = [t:1098..1267] ;12)sentence:[e:1268..1434] = [t:1268..1434] ;13)sentence:[e:1435..1492] = [t:1435..1492] ;14)sentence:[e:1493..1560] = [t:1493..1560] ;15)sentence:[e:1562..1705] = [t:1562..1705] ;16)section:[e:1709..1753] = [t:1709..1753] ;Sentence Matching Errors ;ERROR_Tree section missing entity section[306..532] ;ERROR_Entity section missing treebank section[308..532] ;ERROR_Entity not in any tree[358..367] cyp450 "aromatase" ;ERROR_Entity not in any tree[404..410] substance "agents" ;ERROR_Entity not in any tree[414..423] cyp450 "aromatase" ;ERROR_Entity not in any tree[441..468] cyp450 "aromatase cytochrome P-450" ;ERROR_Entity not in any tree[470..479] cyp450 "P-450arom" ;section 0 Span:0..58 ;Nippon Sanka Fujinka Gakkai Zasshi. 1994 May;46(5):449-56. (SEC (FRAG (NNP:[0..6] Nippon) (NNP:[7..12] Sanka) (NNP:[13..20] Fujinka) (NNP:[21..27] Gakkai) (NNP:[28..34] Zasshi) (.:[34..35] .) (CD:[36..40] 1994) (NNP:[41..44] May) (CD:[44..47] ;46) (-LRB-:[47..48] -LRB-) (CD:[48..49] 5) (-RRB-:[49..50] -RRB-) (::[50..51] :) (CD:[51..55] 449-) (CD:[55..57] 56) (.:[57..58] .))) ;sentence 1 Span:64..120 ;[Regulation of aromatase in human choriocarcinoma cells] ;[79..88]:cyp450:"aromatase" (SENT (NP-HLN (-LRB-:[64..65] -LSB-) (NP (NN:[65..75] Regulation)) (PP (IN:[76..78] of) (NP (NN:[79..88] aromatase))) (PP-LOC (IN:[89..91] in) (NP (JJ:[92..97] human) (NN:[98..113] choriocarcinoma) (NNS:[114..119] cells))) (-RRB-:[119..120] -RSB-))) ;section 2 Span:124..145 ;[Article in Japanese] (SEC (FRAG (-LRB-:[124..125] -LSB-) (NN:[125..132] Article) (IN:[133..135] in) (JJ:[136..144] Japanese) (NNS:[144..145] -RSB-))) ;section 3 Span:149..217 ;Kanno H, Kitawaki J, Maeda K, Kim T, Noguchi T, Yamamoto T, Okada H. (SEC (FRAG (NNP:[149..154] Kanno) (NNP:[155..157] H,) (NNP:[158..166] Kitawaki) (NNP:[167..169] J,) (NNP:[170..175] Maeda) (NNP:[176..178] K,) (NNP:[179..182] Kim) (NNP:[183..184] T) (,:[184..185] ,) (NNP:[186..193] Noguchi) (NNP:[194..195] T) (,:[195..196] ,) (NNP:[197..205] Yamamoto) (NNP:[206..207] T) (,:[207..208] ,) (NNP:[209..214] Okada) (NNP:[215..217] H.))) ;section 4 Span:221..304 ;Department of Obstetrics and Gynecology, Kyoto Prefectural University of ;Medicine. (SEC (FRAG (NNP:[221..231] Department) (IN:[232..234] of) (NNP:[235..245] Obstetrics) (CC:[246..249] and) (NNP:[250..260] Gynecology) (,:[260..261] ,) (NNP:[262..267] Kyoto) (NNP:[268..279] Prefectural) (NNP:[280..290] University) (IN:[291..293] of) (NNP:[295..303] Medicine) (.:[303..304] .))) ;section 5 Span:306..532 ; To examine the mechanism regulating trophoblastic aromatase, we studied the ; effects of various agents on aromatase activity and the aromatase cytochrome ; P-450 (P-450arom) concentration in human choriocarcinoma JEG-3 cells. (SEC (S (S-PRP (NP-SBJ (-NONE-:[306..306] *)) (VP (TO:[308..310] To) (VP (VB:[311..318] examine) (NP (NP (DT:[319..322] the) (NN:[323..332] mechanism)) (VP (VBG:[333..343] regulating) (NP (JJ:[344..357] trophoblastic) (NN:[358..367] aromatase))))))) (,:[367..368] ,) (NP-SBJ (PRP:[369..371] we)) (VP (VBD:[372..379] studied) (NP (NP (DT:[380..383] the) (NNS:[385..392] effects)) (PP (IN:[393..395] of) (NP (JJ:[396..403] various) (NNS:[404..410] agents))) (PP (IN:[411..413] on) (NP (NP (NP (NN:[414..423] aromatase) (NN:[424..432] activity)) (CC:[433..436] and) (NP (DT:[437..440] the) (NML (NML (NN:[441..450] aromatase) (NN:[451..461] cytochrome) (NN:[463..468] P-450)) (NML (-LRB-:[469..470] -LRB-) (NN:[470..479] P-450arom) (-RRB-:[479..480] -RRB-))) (NN:[481..494] concentration))) (PP-LOC (IN:[495..497] in) (NP (JJ:[498..503] human) (NN:[504..519] choriocarcinoma) (NN:[520..525] JEG-3) (NNS:[526..531] cells))))))) (.:[531..532] .))) ;sentence 6 Span:533..613 ;Aromatase activity was assessed by radioassay with [1 beta-3H] ;androstenedione. ;[533..542]:cyp450:"Aromatase" ;[585..612]:substance:"[1 beta-3H] androstenedione" (SENT (S (NP-SBJ-1 (NN:[533..542] Aromatase) (NN:[544..552] activity)) (VP (VBD:[553..556] was) (VP (VBN:[557..565] assessed) (NP-1 (-NONE-:[565..565] *)) (PP-MNR (IN:[566..568] by) (NP (NP (NN:[569..579] radioassay)) (PP (IN:[580..584] with) (NP (NML (-LRB-:[585..586] -LSB-) (CD:[586..587] 1) (NN:[588..595] beta-3H) (-RRB-:[595..596] -RSB-)) (NN:[597..612] androstenedione))))))) (.:[612..613] .))) ;sentence 7 Span:614..737 ;The P-450arom concentration was determined by an enzyme-linked immunosorbent ;assay with specific antibodies to P-450arom. ;[619..628]:cyp450:"P-450arom" ;[664..670]:substance:"enzyme" ;[713..723]:substance:"antibodies" ;[727..736]:cyp450:"P-450arom" (SENT (S (NP-SBJ-1 (DT:[614..617] The) (NN:[619..628] P-450arom) (NN:[629..642] concentration)) (VP (VBD:[643..646] was) (VP (VBN:[647..657] determined) (NP-1 (-NONE-:[657..657] *)) (PP (IN:[658..660] by) (NP-LGS (NP (DT:[661..663] an) (ADJP (NN:[664..670] enzyme) (HYPH:[670..671] -) (VBN:[671..677] linked)) (NN:[678..691] immunosorbent) (NN:[692..697] assay)) (PP (IN:[699..703] with) (NP (NP (JJ:[704..712] specific) (NNS:[713..723] antibodies)) (PP (TO:[724..726] to) (NP (NN:[727..736] P-450arom))))))))) (.:[736..737] .))) ;sentence 8 Span:738..899 ;Human chorionic gonadotropin (hCG) stimulated aromatase activity and ;increased the P-450arom concentration in a concentration-dependent (0.1-100 ;IU/ml) manner. ;[738..766]:substance:"Human chorionic gonadotropin" ;[768..771]:substance:"hCG" ;[785..794]:cyp450:"aromatase" ;[822..831]:cyp450:"P-450arom" ;[877..884]:quantitative-value:"0.1-100" ;[885..890]:quantitative-units:"IU/ml" (SENT (S (NP-SBJ (NP (JJ:[738..743] Human) (JJ:[744..753] chorionic) (NN:[754..766] gonadotropin)) (NP (-LRB-:[767..768] -LRB-) (NN:[768..771] hCG) (-RRB-:[771..772] -RRB-))) (VP (VP (VBD:[774..784] stimulated) (NP (NN:[785..794] aromatase) (NN:[795..803] activity))) (CC:[804..807] and) (VP (VBD:[808..817] increased) (NP (DT:[818..821] the) (NN:[822..831] P-450arom) (NN:[832..845] concentration)) (PP (IN:[846..848] in) (NP (DT:[849..850] a) (ADJP (NN:[852..865] concentration) (HYPH:[865..866] -) (JJ:[866..875] dependent)) (PRN (-LRB-:[876..877] -LRB-) (NP (NP (QP (CD:[877..880] 0.1) (HYPH:[880..881] -) (CD:[881..884] 100)) (NN:[885..887] IU)) (PP (SYM:[887..888] /) (NP (NN:[888..890] ml)))) (-RRB-:[890..891] -RRB-)) (NN:[892..898] manner))))) (.:[898..899] .))) ;sentence 9 Span:900..1096 ;Cholera toxin (CT), an adenylate cyclase activator, stimulated aromatase ;activity and the P-450arom concentration in a concentration-dependent ;(0.1-10ng/ml) and a time-dependent (12-72h) manner. ;[900..913]:substance:"Cholera toxin" ;[915..917]:substance:"CT" ;[923..951]:substance:"adenylate cyclase activator" ;[964..973]:cyp450:"aromatase" ;[991..1000]:cyp450:"P-450arom" ;[1046..1052]:quantitative-value:"0.1-10" ;[1052..1057]:quantitative-units:"ng/ml" ;[1081..1086]:quantitative-value:"12-72" ;[1086..1087]:quantitative-units:"h" (SENT (S (NP-SBJ (NP (NP (NN:[900..907] Cholera) (NN:[908..913] toxin)) (NP (-LRB-:[914..915] -LRB-) (NN:[915..917] CT) (-RRB-:[917..918] -RRB-))) (,:[918..919] ,) (NP (DT:[920..922] an) (NML (NN:[923..932] adenylate) (NN:[934..941] cyclase)) (NN:[942..951] activator))) (,:[951..952] ,) (VP (VBD:[953..963] stimulated) (NP (NP (NN:[964..973] aromatase) (NN:[974..982] activity)) (CC:[983..986] and) (NP (DT:[987..990] the) (NN:[991..1000] P-450arom) (NN:[1001..1014] concentration))) (PP (IN:[1016..1018] in) (NP (NP (DT:[1019..1020] a) (ADJP (NN:[1021..1034] concentration) (HYPH:[1034..1035] -) (JJ:[1035..1044] dependent)) (PRN (-LRB-:[1045..1046] -LRB-) (NP (NP (QP (CD:[1046..1049] 0.1) (HYPH:[1049..1050] -) (CD:[1050..1052] 10)) (NN:[1052..1054] ng)) (PP (SYM:[1054..1055] /) (NP (NN:[1055..1057] ml)))) (-RRB-:[1057..1058] -RRB-)) (NML-1 (-NONE-:[1058..1058] *P*))) (CC:[1059..1062] and) (NP (DT:[1063..1064] a) (ADJP (NN:[1065..1069] time) (HYPH:[1069..1070] -) (JJ:[1070..1079] dependent)) (PRN (-LRB-:[1080..1081] -LRB-) (NP (QP (CD:[1081..1083] 12) (HYPH:[1083..1084] -) (CD:[1084..1086] 72)) (NN:[1086..1087] h)) (-RRB-:[1087..1088] -RRB-)) (NML-1 (NN:[1089..1095] manner)))))) (.:[1095..1096] .))) ;sentence 10 Span:1098..1267 ;12-O-tetradecanoyl phorbol 13-acetate (TPA) (0.1-100ng/ml), a protein-kinase ;C activator, also stimulated aromatase activity and increased the P-450arom ;concentration. ;[1098..1135]:substance:"12-O-tetradecanoyl phorbol 13-acetate" ;[1137..1140]:substance:"TPA" ;[1143..1150]:quantitative-value:"0.1-100" ;[1150..1155]:quantitative-units:"ng/ml" ;[1160..1176]:substance:"protein-kinase C" ;[1178..1187]:substance:"activator" ;[1205..1214]:cyp450:"aromatase" ;[1242..1251]:cyp450:"P-450arom" (SENT (S (NP-SBJ (NP (NP (NN:[1098..1116] 12-O-tetradecanoyl) (NN:[1117..1124] phorbol) (NN:[1125..1135] 13-acetate)) (NP (-LRB-:[1136..1137] -LRB-) (NN:[1137..1140] TPA) (-RRB-:[1140..1141] -RRB-)) (PRN (-LRB-:[1142..1143] -LRB-) (NP (NP (QP (CD:[1143..1146] 0.1) (HYPH:[1146..1147] -) (CD:[1147..1150] 100)) (NN:[1150..1152] ng)) (PP (SYM:[1152..1153] /) (NP (NN:[1153..1155] ml)))) (-RRB-:[1155..1156] -RRB-))) (,:[1156..1157] ,) (NP (DT:[1158..1159] a) (NML (NN:[1160..1167] protein) (HYPH:[1167..1168] -) (NN:[1168..1174] kinase) (NN:[1175..1176] C)) (NN:[1178..1187] activator))) (,:[1187..1188] ,) (ADVP (RB:[1189..1193] also)) (VP (VP (VBD:[1194..1204] stimulated) (NP (NN:[1205..1214] aromatase) (NN:[1215..1223] activity))) (CC:[1224..1227] and) (VP (VBD:[1228..1237] increased) (NP (DT:[1238..1241] the) (NN:[1242..1251] P-450arom) (NN:[1253..1266] concentration)))) (.:[1266..1267] .))) ;sentence 11 Span:1268..1434 ;On the other hand, Ca2+ ionophore A23187, an agent increasing intracellular ;Ca2+ accumulation, inhibited aromatase activity and reduced the P-450arom ;concentration. ;[1287..1301]:substance:"Ca2+ ionophore" ;[1302..1308]:substance:"A23187" ;[1313..1318]:substance:"agent" ;[1345..1349]:substance:"Ca2+" ;[1374..1383]:cyp450:"aromatase" ;[1410..1419]:cyp450:"P-450arom" (SENT (S (PP (IN:[1268..1270] On) (NP (DT:[1271..1274] the) (JJ:[1275..1280] other) (NN:[1281..1285] hand))) (,:[1285..1286] ,) (NP-SBJ (NP (NML (NN:[1287..1290] Ca2) (SYM:[1290..1291] +)) (NN:[1292..1301] ionophore) (NN:[1302..1308] A23187)) (,:[1308..1309] ,) (NP (NP (DT:[1310..1312] an) (NN:[1313..1318] agent)) (VP (VBG:[1319..1329] increasing) (NP (JJ:[1331..1344] intracellular) (NML (NN:[1345..1348] Ca2) (SYM:[1348..1349] +)) (NN:[1350..1362] accumulation))))) (,:[1362..1363] ,) (VP (VP (VBD:[1364..1373] inhibited) (NP (NN:[1374..1383] aromatase) (NN:[1384..1392] activity))) (CC:[1393..1396] and) (VP (VBD:[1397..1404] reduced) (NP (DT:[1405..1408] the) (NN:[1410..1419] P-450arom) (NN:[1420..1433] concentration)))) (.:[1433..1434] .))) ;sentence 12 Span:1435..1492 ;The effects of CT, TPA and Ca2+ ionophore were additive. ;[1450..1452]:substance:"CT" ;[1454..1457]:substance:"TPA" ;[1462..1476]:substance:"Ca2+ ionophore" (SENT (S (NP-SBJ (NP (DT:[1435..1438] The) (NNS:[1439..1446] effects)) (PP (IN:[1447..1449] of) (NP (NP (NN:[1450..1452] CT)) (,:[1452..1453] ,) (NP (NN:[1454..1457] TPA)) (CC:[1458..1461] and) (NP (NML (NN:[1462..1465] Ca2) (SYM:[1465..1466] +)) (NN:[1467..1476] ionophore))))) (VP (VBD:[1477..1481] were) (ADJP-PRD (JJ:[1483..1491] additive))) (.:[1491..1492] .))) ;sentence 13 Span:1493..1560 ;Aromatase activity was correlated with the P-450arom concentration. ;[1493..1502]:cyp450:"Aromatase" ;[1536..1545]:cyp450:"P-450arom" (SENT (S (NP-SBJ-1 (NN:[1493..1502] Aromatase) (NN:[1503..1511] activity)) (VP (VBD:[1512..1515] was) (VP (VBN:[1516..1526] correlated) (NP-1 (-NONE-:[1526..1526] *)) (PP-CLR (IN:[1527..1531] with) (NP (DT:[1532..1535] the) (NN:[1536..1545] P-450arom) (NN:[1546..1559] concentration))))) (.:[1559..1560] .))) ;sentence 14 Span:1562..1705 ;These results suggest that in JEG-3 cells the signal transduction system ;modulates aromatase activity by changing the P-450arom concentration. ;[1646..1655]:cyp450:"aromatase" ;[1681..1690]:cyp450:"P-450arom" (SENT (S (NP-SBJ (DT:[1562..1567] These) (NNS:[1568..1575] results)) (VP (VBP:[1576..1583] suggest) (SBAR (IN:[1584..1588] that) (S (PP-LOC (IN:[1589..1591] in) (NP (NN:[1592..1597] JEG-3) (NNS:[1598..1603] cells))) (NP-SBJ (DT:[1604..1607] the) (NN:[1608..1614] signal) (NN:[1615..1627] transduction) (NN:[1628..1634] system)) (VP (VBZ:[1636..1645] modulates) (NP (NN:[1646..1655] aromatase) (NN:[1656..1664] activity)) (PP-MNR (IN:[1665..1667] by) (S-NOM (NP-SBJ (-NONE-:[1667..1667] *)) (VP (VBG:[1668..1676] changing) (NP (DT:[1677..1680] the) (NN:[1681..1690] P-450arom) (NN:[1691..1704] concentration))))))))) (.:[1704..1705] .))) ;section 15 Span:1709..1753 ;PMID: 8182306 [PubMed - indexed for MEDLINE] (SEC (FRAG (NNP:[1709..1713] PMID) (::[1713..1714] :) (CD:[1715..1722] 8182306) (NN:[1723..1724] -LSB-) (NNP:[1724..1730] PubMed) (::[1731..1732] -) (NN:[1733..1740] indexed) (IN:[1741..1744] for) (NNP:[1745..1753] MEDLINE-RSB-)))