J Cardiovasc Pharmacol. 1990 Sep;16(3):438-43.  

Cytochrome P450-dependent arachidonic acid metabolites, 19- and
20-hydroxyeicosatetraenoic acids, enhance sodium-potassium ATPase activity in
vascular smooth muscle.

Escalante B, Sessa WC, Falck JR, Yadagiri P, Schwartzman ML.

Department of Pharmacology, New York Medical College, Valhalla 10595.

Several cytochrome P450-dependent arachidonic acid metabolites have been shown
to affect Na+,K(+)-ATPase activity. In the present study, we tested the effect
of omega- and omega - 1-hydroxylated products, i.e., 19- and
20-hydroxyeicosatetraenoic acids (19- and 20-HETE), on the K-induced relaxation
in rat aortic rings. 19-HETE and 20-HETE increased the magnitude of the
potassium-induced relaxation in a dose-dependent fashion (10(-7)-10(-5) M). The
inhibitory effect of ouabain on the potassium-induced relaxation was reversed by
both 19- and 20-HETE. In addition, indomethacin fully inhibited the stimulatory
effect of 19- and 20-HETE on relaxation induced by potassium. Vascular
ouabain-sensitive 86Rb uptake was also increased by 19- and 20-HETE. These
observations suggest that 19- and 20-HETE stimulate vascular Na+,K(+)-ATPase via
their conversion by cyclooxygenase to prostaglandin-like material.

PMID: 1700215 [PubMed - indexed for MEDLINE]