Jpn J Pharmacol. 1981 Feb;31(1):107-16.  

Carbon tetrachloride-induced loss of microsomal glucose 6-phosphatase and
cytochrome P-450 in vitro.

Masuda Y.

Rat liver microsomes were incubated in the presence of NADPH and CCl4 under
various conditions, and losses of glucose 6-phosphatase (G-6-Pase) and
cytochrome P-450 were examined in terms of lipid peroxidation and CCl4
metabolism. Loss of G-6-Pase activity correlated well with the enhancement of
lipid peroxidation. Loss of cytochrome P-450 was also dependent on the lipid
peroxidation, under aerobic conditions. However, the cytochrome was destroyed
under anaerobic conditions in which lipid peroxidation and loss of G-6-Pase were
greatly suppressed. This anaerobic loss of cytochrome P-450 may be linked with
the metabolism of CCl4 by this hemoprotein, as evidenced by the observation that
CCl4 metabolism occurred only under anaerobic conditions and was inhibited by
carbon monoxide accompanied by the suppression of the loss of cytochrome P-450.

PMID: 6265675 [PubMed - indexed for MEDLINE]