Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998
Nov;121(1-3):333-8.  

Cytochrome P450 system in the hepatopancreas of the red swamp crayfish
Procambarus clarkii: a field study.

Porte C, Escartin E.

Environmental Chemistry Department, CID, CSIC, Barcelona, Spain.
cpvqam@cid.csic.es

Cytochrome P450 monooxygenase system and acetylcholinesterase (AChE) activity
were studied in the red swamp crayfish Procambarus clarkii sampled from the Ebro
Delta before and after spraying of the area with fenitrothion. Analysis of
hepatopancreas microsomes revealed an increase of 7-ethoxyresorufin O-deethylase
(EROD) activity in organisms sampled after spraying. AChE activity was
significantly inhibited in the same specimens, corroborating that these
organisms were exposed to fenitrothion, and the usefulness of AChE as a marker
of pesticide poisoning in P. clarkii. Crayfish from a reference site were
analyzed and the results compared with those obtained in organisms from the Ebro
Delta. The ability of P. clarkii to metabolize the organophosphorus pesticide
fenitrothion was determined in vitro by using microsomal fractions isolated from
hepatopancreas. The results pointed out the existence of an oxidative
metabolism, which was inhibited by different P450 inhibitors (clotrimazole,
alpha-naphthoflavone and N-benzylimidazole) and not affected by methimazole.

PMID: 9972475 [PubMed - indexed for MEDLINE]