Biochem Biophys Res Commun. 1988 Apr 29;152(2):843-8.  

A male-specific renal cytochrome P-450 isozyme(s) responsible for mutagenic
activation of 3-methoxy-4-aminoazobenzene in mice.

Degawa M, Namiki M, Miura S, Ueno H, Hashimoto Y.

Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.

Renal microsomes from male mice (BALB/c, DBA/2 and BALB/c x DBA/2 F1) showed
about 10-fold greater activity for mediating mutagenic activation of
3-methoxy-4-aminoazobenzene (3-MeO-AAB) toward Salmonella typhimurium TA98 than
did the corresponding hepatic microsomes, as compared on the basis of nmol of
microsomal cytochrome P-450. On the other hand, female renal microsomes and
other extrahepatic microsomes (lung, small intestine and colon) in both sexes of
mice showed little or no activity for converting 3-MeO-AAB to mutagen(s). The
mutagenic activation of 3-MeO-AAB with the male renal enzyme(s) was definitely
inhibited by cytochrome P-450 inhibitors, 7,8-benzoflavone and SKF 525A. All
these findings suggest that in mice, there is a male-specific renal 3-MeO-AAB
activation enzyme(s), a cytochrome P-450 isozyme(s), which is different, at
least in proportion and/or in nature, from hepatic cytochrome P-450 isozymes.

PMID: 3365254 [PubMed - indexed for MEDLINE]