Am J Obstet Gynecol. 1990 Nov;163(5 Pt 1):1546-51.  

Inhibition of aromatase activity by 8-bromo-cyclic adenosine monophosphate in
cultured first-trimester human trophoblast.

Rodway MR, Yuen BH, Leung PC.

Department of Obstestrics and Gynecology, University of British Columbia, Grace
Hospital, Vancouver, Canada.

We have previously shown that 8-bromo-adenosine-3':5'-cyclic monophosphate
inhibits production of estradiol from androgen precursors in trophoblast
isolated from term placentas. In these experiments we sought to evaluate the
effect of 8-bromo-adenosine-3':5'-cyclic monophosphate on aromatase activity in
first-trimester trophoblast culture. Trophoblast cells were enzyme dispersed
from first-trimester placentas and purified on a Percoll gradient. On the third
and fourth day after dispersion cultures were treated with androstenedione or
testosterone with and without 2 mmol/L 8-bromo-adenosine-3':5'-cyclic
monophosphate. The quantity of estradiol, estrone, progesterone, human placental
lactogen, and human chorionic gonadotropin was determined by radioimmunoassay.
We concluded that in first-trimester trophoblast, as previously reported in term
trophoblast, 8-bromo-adenosine-3':5'-cyclic monophosphate inhibited the
production of estradiol from the supplied androgen precursors. Human chorionic
gonadotropin and human placental lactogen production was stimulated by
8-bromo-adenosine-3':5'-cyclic monophosphate. Progesterone production was either
stimulated or was unchanged by the presence of 8-bromo-adenosine-3':5'-cyclic
monophosphate.

PMID: 2240104 [PubMed - indexed for MEDLINE]